This is a Validated Antibody Database (VAD) review about human SETD7, based on 9 published articles (read how Labome selects the articles), using SETD7 antibody in all methods. It is aimed to help Labome visitors find the most suited SETD7 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SETD7 synonym: KMT7; SET7; SET7/9; SET9; histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; lysine N-methyltransferase 7

Knockout validation
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
  • immunoprecipitation; human; fig 1
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 1
Cell Signaling Technology SETD7 antibody (Cell Signaling Technology, 2813) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1), in immunoprecipitation on human samples (fig 1), in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 1). Nat Commun (2016) ncbi
Abcam
rabbit monoclonal (EPR5574)
  • western blot; mouse; 1:500; fig 2
Abcam SETD7 antibody (Abcam, ab124708) was used in western blot on mouse samples at 1:500 (fig 2). Nat Commun (2016) ncbi
Bethyl
rabbit polyclonal
  • chromatin immunoprecipitation; hamsters; fig 6
  • western blot; hamsters; fig 6
Bethyl SETD7 antibody (Bethyl, A301-747A) was used in chromatin immunoprecipitation on hamsters samples (fig 6) and in western blot on hamsters samples (fig 6). Mol Cell Endocrinol (2016) ncbi
rabbit polyclonal
  • western blot; hamsters; fig 2
  • western blot; mouse; fig 6
Bethyl SETD7 antibody (Bethyl, A301747A) was used in western blot on hamsters samples (fig 2) and in western blot on mouse samples (fig 6). Cell Cycle (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (s4E5)
  • western blot; mouse; loading ...; fig 5a
Santa Cruz Biotechnology SETD7 antibody (Santa Cruz, sc-56774) was used in western blot on mouse samples (fig 5a). Cell Commun Signal (2014) ncbi
Invitrogen
mouse monoclonal
  • immunoprecipitation; human; 2 ug
In order to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality, Invitrogen SETD7 antibody (life tech, 730055) was used in immunoprecipitation on human samples at 2 ug. Nat Methods (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3c
Cell Signaling Technology SETD7 antibody (Cell Signaling, 2813) was used in immunohistochemistry - frozen section on mouse samples (fig s3c). J Cell Biol (2018) ncbi
rabbit monoclonal (C24B1)
  • western blot; pig; loading ...; fig 5b
Cell Signaling Technology SETD7 antibody (Cell Signal, 2825) was used in western blot on pig samples (fig 5b). Oncotarget (2017) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
  • immunoprecipitation; human; fig 1
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 1
Cell Signaling Technology SETD7 antibody (Cell Signaling Technology, 2813) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1), in immunoprecipitation on human samples (fig 1), in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 1). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3
In order to test if lys methylation of Pdx1 by Set7/9 affects Pdx1 transcriptional activity, Cell Signaling Technology SETD7 antibody (Cell Signaling Technology, 2813) was used in western blot on mouse samples (fig 3). J Biol Chem (2015) ncbi
Articles Reviewed
  1. Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  2. Cai C, Qian L, Jiang S, Sun Y, Wang Q, Ma D, et al. Loss-of-function myostatin mutation increases insulin sensitivity and browning of white fat in Meishan pigs. Oncotarget. 2017;8:34911-34922 pubmed publisher
  3. Fulcoli F, Franzese M, Liu X, Zhang Z, Angelini C, Baldini A. Rebalancing gene haploinsufficiency in vivo by targeting chromatin. Nat Commun. 2016;7:11688 pubmed publisher
  4. Wang W, Shi Q, Guo T, Yang Z, Jia Z, Chen P, et al. PDX1 and ISL1 differentially coordinate with epigenetic modifications to regulate insulin gene expression in varied glucose concentrations. Mol Cell Endocrinol. 2016;428:38-48 pubmed publisher
  5. Kim Y, Nam H, Lee J, Park D, Kim C, Yu Y, et al. Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis. Nat Commun. 2016;7:10347 pubmed publisher
  6. Yang Z, Zhang Q, Lu Q, Jia Z, Chen P, Ma K, et al. ISL-1 promotes pancreatic islet cell proliferation by forming an ISL-1/Set7/9/PDX-1 complex. Cell Cycle. 2015;14:3820-9 pubmed publisher
  7. Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, et al. Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation. Nat Methods. 2015;12:725-31 pubmed publisher
  8. Maganti A, Maier B, Tersey S, Sampley M, Mosley A, Özcan S, et al. Transcriptional activity of the islet β cell factor Pdx1 is augmented by lysine methylation catalyzed by the methyltransferase Set7/9. J Biol Chem. 2015;290:9812-22 pubmed publisher
  9. Bravard A, Vial G, Chauvin M, Rouille Y, Bailleul B, Vidal H, et al. FTO contributes to hepatic metabolism regulation through regulation of leptin action and STAT3 signalling in liver. Cell Commun Signal. 2014;12:4 pubmed publisher