This is a Validated Antibody Database (VAD) review about human SCA2, based on 13 published articles (read how Labome selects the articles), using SCA2 antibody in all methods. It is aimed to help Labome visitors find the most suited SCA2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SCA2 synonym: ATX2; SCA2; TNRC13; ataxin-2; spinocerebellar ataxia type 2 protein; trinucleotide repeat-containing gene 13 protein

Bethyl
rabbit polyclonal
In order to develop reagents to study RNA binding proteins, Bethyl SCA2 antibody (Bethyl, A301-118A) was used . Mol Cell (2016) ncbi
Proteintech Group
rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 1s2e
Proteintech Group SCA2 antibody (Proteintech, 21776-1-AP) was used in immunohistochemistry on human samples (fig 1s2e). elife (2019) ncbi
BD Biosciences
mouse monoclonal (22/Ataxin-2)
  • immunohistochemistry; human; 1:4000; loading ...; fig 1a
BD Biosciences SCA2 antibody (BD Biosciences, 22) was used in immunohistochemistry on human samples at 1:4000 (fig 1a). Nat Commun (2018) ncbi
mouse monoclonal (22/Ataxin-2)
  • western blot; human; 1:4000; loading ...; fig 2b
BD Biosciences SCA2 antibody (BD Biosciences, 611378) was used in western blot on human samples at 1:4000 (fig 2b). Nature (2017) ncbi
mouse monoclonal (22/Ataxin-2)
  • immunocytochemistry; human; loading ...; fig 2e
  • western blot; human; 1:1000; loading ...; fig 2d
BD Biosciences SCA2 antibody (BD, 611378) was used in immunocytochemistry on human samples (fig 2e) and in western blot on human samples at 1:1000 (fig 2d). Nature (2017) ncbi
mouse monoclonal (22/Ataxin-2)
  • immunocytochemistry; human; loading ...; fig 6g
BD Biosciences SCA2 antibody (BD Biosciences, 611378) was used in immunocytochemistry on human samples (fig 6g). Mol Cell (2017) ncbi
mouse monoclonal (22/Ataxin-2)
  • western blot; mouse; 1:3000; fig 1
  • western blot; human; 1:3000; fig 1
BD Biosciences SCA2 antibody (BD Biosciences, 611378) was used in western blot on mouse samples at 1:3000 (fig 1) and in western blot on human samples at 1:3000 (fig 1). PLoS Genet (2015) ncbi
mouse monoclonal (22/Ataxin-2)
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 2
  • immunoprecipitation; mouse; 1:400; loading ...; fig 2a
  • western blot; mouse; 1:400; loading ...; fig 1g,1h
  • immunoprecipitation; human; 1:400; loading ...; fig 1g
  • western blot; human; 1:400; loading ...; fig 1g
BD Biosciences SCA2 antibody (BD Biosciences, 611378) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 2), in immunoprecipitation on mouse samples at 1:400 (fig 2a), in western blot on mouse samples at 1:400 (fig 1g,1h), in immunoprecipitation on human samples at 1:400 (fig 1g) and in western blot on human samples at 1:400 (fig 1g). PLoS ONE (2015) ncbi
mouse monoclonal (22/Ataxin-2)
  • immunocytochemistry; human; 1:200
In order to examine the methylation state of ATXN2L and its contribution to ATXN2L localization, BD Biosciences SCA2 antibody (BD Biosciences, 611378) was used in immunocytochemistry on human samples at 1:200. Exp Cell Res (2015) ncbi
mouse monoclonal (22/Ataxin-2)
  • immunohistochemistry - paraffin section; human; 1:200
In order to investigate the association of eosinophilic neuronal cytoplasmic inclusions with stress granules and autophagy in two clinical cases, BD Biosciences SCA2 antibody (BD Biosciences, 22/Ataxin-2) was used in immunohistochemistry - paraffin section on human samples at 1:200. Neuropathology (2014) ncbi
mouse monoclonal (22/Ataxin-2)
  • immunocytochemistry; human; 1:200
BD Biosciences SCA2 antibody (BD, 611378) was used in immunocytochemistry on human samples at 1:200. Nucleic Acids Res (2014) ncbi
mouse monoclonal (22/Ataxin-2)
  • immunocytochemistry; human; fig 7
BD Biosciences SCA2 antibody (BD, 611378) was used in immunocytochemistry on human samples (fig 7). J Virol (2011) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunocytochemistry; human; 1:250; fig 4
In order to investigate the connection between calorie restriction and magnesium, Sigma-Aldrich SCA2 antibody (Sigma, HPA021146) was used in immunocytochemistry on human samples at 1:250 (fig 4). Nucleic Acids Res (2016) ncbi
Articles Reviewed
  1. Zhang P, Fan B, Yang P, Temirov J, Messing J, Kim H, et al. Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. elife. 2019;8: pubmed publisher
  2. Paul S, Dansithong W, Figueroa K, Scoles D, Pulst S. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun. 2018;9:3648 pubmed publisher
  3. Scoles D, Meera P, Schneider M, Paul S, Dansithong W, Figueroa K, et al. Antisense oligonucleotide therapy for spinocerebellar ataxia type 2. Nature. 2017;544:362-366 pubmed publisher
  4. Becker L, Huang B, Bieri G, Ma R, Knowles D, Jafar Nejad P, et al. Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. Nature. 2017;544:367-371 pubmed publisher
  5. Boeynaems S, Bogaert E, Kovacs D, Konijnenberg A, Timmerman E, Volkov A, et al. Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics. Mol Cell. 2017;65:1044-1055.e5 pubmed publisher
  6. Abraham K, Chan J, Salvi J, Ho B, Hall A, Vidya E, et al. Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acids Res. 2016;44:8870-8884 pubmed
  7. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  8. Dansithong W, Paul S, Figueroa K, Rinehart M, Wiest S, Pflieger L, et al. Ataxin-2 regulates RGS8 translation in a new BAC-SCA2 transgenic mouse model. PLoS Genet. 2015;11:e1005182 pubmed publisher
  9. Halbach M, Stehning T, Damrath E, Jendrach M, Åžen N, BaÅŸak A, et al. Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated. PLoS ONE. 2015;10:e0121089 pubmed publisher
  10. Kaehler C, Guenther A, Uhlich A, Krobitsch S. PRMT1-mediated arginine methylation controls ATXN2L localization. Exp Cell Res. 2015;334:114-25 pubmed publisher
  11. Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  12. Kaehler C, Isensee J, Hucho T, Lehrach H, Krobitsch S. 5-Fluorouracil affects assembly of stress granules based on RNA incorporation. Nucleic Acids Res. 2014;42:6436-47 pubmed publisher
  13. Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, et al. Hepatitis C virus hijacks P-body and stress granule components around lipid droplets. J Virol. 2011;85:6882-92 pubmed publisher