This is a Validated Antibody Database (VAD) review about human SAMHD1, based on 10 published articles (read how Labome selects the articles), using SAMHD1 antibody in all methods. It is aimed to help Labome visitors find the most suited SAMHD1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SAMHD1 synonym: CHBL2; DCIP; HDDC1; MOP-5; SBBI88; deoxynucleoside triphosphate triphosphohydrolase SAMHD1; SAM domain and HD domain 1; SAM domain and HD domain-containing protein 1; dNTPase; dendritic cell-derived IFNG-induced protein; monocyte protein 5

Knockout validation
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2a
  • immunohistochemistry; human; 1:3000; loading ...; fig 4d
In order to demonstrate that SAMHD1 reduces nucleoside analog cytarabine cytotoxicity in acute myeloid leukemia cells, Proteintech Group SAMHD1 antibody (Proteintech, 12586-1-AP) was used in western blot knockout validation on human samples at 1:1000 (fig 2a) and in immunohistochemistry on human samples at 1:3000 (fig 4d). Nat Med (2017) ncbi
OriGene
mouse monoclonal (OTI1A1)
  • immunocytochemistry; human; loading ...; fig 3a
  • western blot; human; loading ...; fig 5d
OriGene SAMHD1 antibody (Origene, 1A1) was used in immunocytochemistry on human samples (fig 3a) and in western blot on human samples (fig 5d). AIDS Res Hum Retroviruses (2017) ncbi
mouse monoclonal (OTI2B5)
  • immunocytochemistry; human; loading ...; fig 10
  • western blot; human; loading ...; fig s1
OriGene SAMHD1 antibody (ORIGENE, TA502085) was used in immunocytochemistry on human samples (fig 10) and in western blot on human samples (fig s1). PLoS Genet (2015) ncbi
mouse monoclonal (OTI1F6)
  • western blot; human; loading ...; fig s4a
OriGene SAMHD1 antibody (Origene, 1F6) was used in western blot on human samples (fig s4a). PLoS Pathog (2015) ncbi
Abcam
mouse monoclonal (OTI1A1)
  • western blot; mouse; loading ...; fig 1a
Abcam SAMHD1 antibody (Abcam, OTI1A1) was used in western blot on mouse samples (fig 1a). Proc Natl Acad Sci U S A (2018) ncbi
mouse polyclonal
  • western blot; human; fig 3g
Abcam SAMHD1 antibody (Abcam, ab67820) was used in western blot on human samples (fig 3g). EMBO J (2017) ncbi
Proteintech Group
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 2000 ng/ml; loading ...; fig 2d
In order to show that the deoxynucleoside triphosphate triphosphohydrolase SAM domain and HD domain 1 promotes the detoxification of intracellular cytostatic deoxycytidine analog cytarabine triphosphate pools, Proteintech Group SAMHD1 antibody (Proteintech, 12586-1-A) was used in immunohistochemistry - paraffin section on human samples at 2000 ng/ml (fig 2d). Nat Med (2017) ncbi
rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2a
  • immunohistochemistry; human; 1:3000; loading ...; fig 4d
In order to demonstrate that SAMHD1 reduces nucleoside analog cytarabine cytotoxicity in acute myeloid leukemia cells, Proteintech Group SAMHD1 antibody (Proteintech, 12586-1-AP) was used in western blot knockout validation on human samples at 1:1000 (fig 2a) and in immunohistochemistry on human samples at 1:3000 (fig 4d). Nat Med (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:100
Proteintech Group SAMHD1 antibody (Proteintech, 12586-1-AP) was used in western blot on human samples at 1:100. Antimicrob Agents Chemother (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 2d
Cell Signaling Technology SAMHD1 antibody (CST, 12361) was used in western blot on human samples (fig 2d). Sci Rep (2017) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; human; 1 ug/ml; fig s4
Sigma-Aldrich SAMHD1 antibody (Sigma, SAB2102077) was used in western blot on human samples at 1 ug/ml (fig s4). Nat Commun (2015) ncbi
Articles Reviewed
  1. Thientosapol E, Bosnjak D, Durack T, Stevanovski I, van Geldermalsen M, Holst J, et al. SAMHD1 enhances immunoglobulin hypermutation by promoting transversion mutation. Proc Natl Acad Sci U S A. 2018;115:4921-4926 pubmed publisher
  2. Cenker J, Stultz R, McDonald D. Brain Microglial Cells Are Highly Susceptible to HIV-1 Infection and Spread. AIDS Res Hum Retroviruses. 2017;33:1155-1165 pubmed publisher
  3. Mlcochova P, Sutherland K, Watters S, Bertoli C, de Bruin R, Rehwinkel J, et al. A G1-like state allows HIV-1 to bypass SAMHD1 restriction in macrophages. EMBO J. 2017;36:604-616 pubmed publisher
  4. Prasad A, Kulkarni R, Jiang S, Groopman J. Cocaine Enhances DC to T-cell HIV-1 Transmission by Activating DC-SIGN/LARG/LSP1 Complex and Facilitating Infectious Synapse Formation. Sci Rep. 2017;7:40648 pubmed publisher
  5. Herold N, Rudd S, Ljungblad L, Sanjiv K, Myrberg I, Paulin C, et al. Targeting SAMHD1 with the Vpx protein to improve cytarabine therapy for hematological malignancies. Nat Med. 2017;23:256-263 pubmed publisher
  6. Schneider C, Oellerich T, Baldauf H, Schwarz S, Thomas D, Flick R, et al. SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia. Nat Med. 2017;23:250-255 pubmed publisher
  7. Hu S, Li J, Xu F, Mei S, Le Duff Y, Yin L, et al. SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation. PLoS Genet. 2015;11:e1005367 pubmed publisher
  8. Adoro S, Cubillos Ruiz J, Chen X, Deruaz M, Vrbanac V, Song M, et al. IL-21 induces antiviral microRNA-29 in CD4 T cells to limit HIV-1 infection. Nat Commun. 2015;6:7562 pubmed publisher
  9. Martin Gayo E, Buzón M, Ouyang Z, Hickman T, Cronin J, Pimenova D, et al. Potent Cell-Intrinsic Immune Responses in Dendritic Cells Facilitate HIV-1-Specific T Cell Immunity in HIV-1 Elite Controllers. PLoS Pathog. 2015;11:e1004930 pubmed publisher
  10. Ballana E, Badia R, Terradas G, Torres Torronteras J, Ruiz A, Pauls E, et al. SAMHD1 specifically affects the antiviral potency of thymidine analog HIV reverse transcriptase inhibitors. Antimicrob Agents Chemother. 2014;58:4804-13 pubmed publisher