This is a Validated Antibody Database (VAD) review about human Rap1, based on 11 published articles (read how Labome selects the articles), using Rap1 antibody in all methods. It is aimed to help Labome visitors find the most suited Rap1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rap1 synonym: C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21

Santa Cruz Biotechnology
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...; fig 2b
Santa Cruz Biotechnology Rap1 antibody (Santa Cruz, sc-398755) was used in western blot on mouse samples at 1:1000 (fig 2b). JCI Insight (2021) ncbi
  • western blot; human; fig 3a
In order to study the role of statin and ERK5 in tight junction formation and function in endothelial cells, Santa Cruz Biotechnology Rap1 antibody (Santa Cruz, SC-1482) was used in western blot on human samples (fig 3a). J Cell Physiol (2018) ncbi
  • western blot; mouse; 1:1000; fig 2
Santa Cruz Biotechnology Rap1 antibody (santa Cruz, sc1482) was used in western blot on mouse samples at 1:1000 (fig 2). Small Gtpases (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (26B4)
  • western blot; human; 1:500; loading ...; fig 5b
Cell Signaling Technology Rap1 antibody (CST, 2399) was used in western blot on human samples at 1:500 (fig 5b). Sci Rep (2021) ncbi
domestic rabbit monoclonal (26B4)
  • western blot; human; loading ...; fig 6
In order to analyze the capacity of monocytes, neutrophils, and CD4 T cells to serve as accessory cells for gamma delta T cell activation, Cell Signaling Technology Rap1 antibody (Cell signaling, 2399) was used in western blot on human samples (fig 6). J Immunol (2016) ncbi
domestic rabbit monoclonal (26B4)
  • western blot; mouse; 1:1000; fig 2
Cell Signaling Technology Rap1 antibody (Cell Signaling Tech, 2399S) was used in western blot on mouse samples at 1:1000 (fig 2). Small Gtpases (2015) ncbi
domestic rabbit monoclonal (26B4)
  • western blot; African green monkey; fig 3
Cell Signaling Technology Rap1 antibody (Cell Signaling Tech, 2399) was used in western blot on African green monkey samples (fig 3). Mol Biol Cell (2015) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:25; fig 5
Cell Signaling Technology Rap1 antibody (Cell Signaling, 4938 S) was used in immunocytochemistry on human samples at 1:25 (fig 5). Oncogene (2016) ncbi
domestic rabbit monoclonal (26B4)
  • immunohistochemistry - paraffin section; mouse; 1:200
  • western blot; mouse
In order to study the role of the Rac1 GTPase the shaping of the ocular lens, Cell Signaling Technology Rap1 antibody (Cell Signaling Technologies,, 2399) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 and in western blot on mouse samples . Dev Biol (2011) ncbi
BD Biosciences
mouse monoclonal (3/Rap1)
  • western blot; rat; 1:2000; loading ...; fig 1d
BD Biosciences Rap1 antibody (BD Biosciences, 610195) was used in western blot on rat samples at 1:2000 (fig 1d). Neuron (2018) ncbi
mouse monoclonal (3/Rap1)
  • western blot; mouse; fig 2
  • western blot; human; fig 6
BD Biosciences Rap1 antibody (BD Transduction Laboratories, 610195) was used in western blot on mouse samples (fig 2) and in western blot on human samples (fig 6). Mol Ther Methods Clin Dev (2016) ncbi
mouse monoclonal (3/Rap1)
  • western blot; human
BD Biosciences Rap1 antibody (BD Biosciences, 610195) was used in western blot on human samples . Mol Cell Biol (2014) ncbi
Articles Reviewed
  1. Shelton W, Thomas S, Alexander H, Thomes C, Conway D, Dubash A. Desmoglein-2 harnesses a PDZ-GEF2/Rap1 signaling axis to control cell spreading and focal adhesions independent of cell-cell adhesion. Sci Rep. 2021;11:13295 pubmed publisher
  2. Kaneko K, Lin H, Fu Y, Saha P, De la Puente Gomez A, Xu Y, et al. Rap1 in the VMH regulates glucose homeostasis. JCI Insight. 2021;6: pubmed publisher
  3. Zhang L, Zhang P, Wang G, Zhang H, Zhang Y, Yu Y, et al. Ras and Rap Signal Bidirectional Synaptic Plasticity via Distinct Subcellular Microdomains. Neuron. 2018;98:783-800.e4 pubmed publisher
  4. Wilkinson E, Sidaway J, Cross M. Statin regulated ERK5 stimulates tight junction formation and reduces permeability in human cardiac endothelial cells. J Cell Physiol. 2018;233:186-200 pubmed publisher
  5. Nerdal P, Peters C, Oberg H, Zlatev H, Lettau M, Quabius E, et al. Butyrophilin 3A/CD277-Dependent Activation of Human ?? T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations. J Immunol. 2016;197:3059-3068 pubmed
  6. Wang H, Han X, Bretz C, Becker S, Gambhir D, Smith G, et al. Retinal pigment epithelial cell expression of active Rap 1a by scAAV2 inhibits choroidal neovascularization. Mol Ther Methods Clin Dev. 2016;3:16056 pubmed publisher
  7. Ali N, Jurczyluk J, Shay G, Tnimov Z, Alexandrov K, Munoz M, et al. A highly sensitive prenylation assay reveals in vivo effects of bisphosphonate drug on the Rab prenylome of macrophages outside the skeleton. Small Gtpases. 2015;6:202-11 pubmed publisher
  8. Scott D, Tolbert C, Graham D, Wittchen E, Bear J, Burridge K. N-glycosylation controls the function of junctional adhesion molecule-A. Mol Biol Cell. 2015;26:3205-14 pubmed publisher
  9. Ho J, Nadeem A, Rydström A, Puthia M, Svanborg C. Targeting of nucleotide-binding proteins by HAMLET--a conserved tumor cell death mechanism. Oncogene. 2016;35:897-907 pubmed publisher
  10. Kloog Y, Mor A. Cytotoxic-T-lymphocyte antigen 4 receptor signaling for lymphocyte adhesion is mediated by C3G and Rap1. Mol Cell Biol. 2014;34:978-88 pubmed publisher
  11. Maddala R, Chauhan B, Walker C, Zheng Y, Robinson M, Lang R, et al. Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival. Dev Biol. 2011;360:30-43 pubmed publisher