This is a Validated Antibody Database (VAD) review about human RWDD2A, based on 15 published articles (read how Labome selects the articles), using RWDD2A antibody in all methods. It is aimed to help Labome visitors find the most suited RWDD2A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RWDD2A synonym: RWDD2; dJ747H23.2; RWD domain-containing protein 2A; RWD domain containing 2

Sigma-Aldrich
mouse monoclonal (5C5)
  • western blot; mouse; 1:2000; loading ...; fig s4d
In order to discover that a common null polymorphism (R577X) in ACTN3 results in significantly reduced muscle strength in patients with Duchenne muscular dystrophy, Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in western blot on mouse samples at 1:2000 (fig s4d). Nat Commun (2017) ncbi
mouse monoclonal (5C5)
  • western blot; human; 1:5000; fig 5
Sigma-Aldrich RWDD2A antibody (Sigma, A-2172) was used in western blot on human samples at 1:5000 (fig 5). Mol Metab (2016) ncbi
mouse monoclonal (5C5)
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 6a
In order to describe a preclinical platform for validation of new therapies in human heart tissue, Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 6a). Sci Rep (2016) ncbi
mouse monoclonal (5C5)
  • immunohistochemistry - paraffin section; mouse; 1:15-1:100; fig 3
Sigma-Aldrich RWDD2A antibody (Sigma Aldrich, A2172) was used in immunohistochemistry - paraffin section on mouse samples at 1:15-1:100 (fig 3). Oxid Med Cell Longev (2016) ncbi
mouse monoclonal (5C5)
  • western blot; human; 1:10,000; fig 3
Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in western blot on human samples at 1:10,000 (fig 3). Oncotarget (2016) ncbi
mouse monoclonal (5C5)
  • western blot; rat; 1:1000; fig 4
Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in western blot on rat samples at 1:1000 (fig 4). Int J Mol Med (2015) ncbi
mouse monoclonal (5C5)
  • western blot; mouse; 1:5000; fig 1
Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in western blot on mouse samples at 1:5000 (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (5C5)
  • immunocytochemistry; mouse; 1:400
Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in immunocytochemistry on mouse samples at 1:400. J Physiol (2015) ncbi
mouse monoclonal (5C5)
  • western blot; mouse; 1:40,000; fig 4
In order to examine the contribution of neuronal NOSmu on skeletal muscle glucose uptake during ex vivo contraction, Sigma-Aldrich RWDD2A antibody (Sigma Aldrich, A2172) was used in western blot on mouse samples at 1:40,000 (fig 4). J Appl Physiol (1985) (2015) ncbi
mouse monoclonal (5C5)
  • western blot; human; 1:5000
In order to show that the loss of claudin-5 in cardiomyocytes and endothelial cells is prevalent in human heart failure, Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in western blot on human samples at 1:5000. Cardiovasc Pathol (2015) ncbi
mouse monoclonal (5C5)
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 4
In order to study the effect of mild coxsackievirus B infection on the heart, Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4). PLoS Pathog (2014) ncbi
mouse monoclonal (5C5)
  • western blot; rat; 1:5000
Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in western blot on rat samples at 1:5000. PLoS ONE (2014) ncbi
mouse monoclonal (5C5)
  • western blot; mouse; 1:2000; fig 7
Sigma-Aldrich RWDD2A antibody (Sigma, 5C5) was used in western blot on mouse samples at 1:2000 (fig 7). Hum Mol Genet (2014) ncbi
mouse monoclonal (5C5)
  • immunocytochemistry; common platanna; 1:500; tbl 1
Sigma-Aldrich RWDD2A antibody (Sigma, A2172) was used in immunocytochemistry on common platanna samples at 1:500 (tbl 1). Methods (2014) ncbi
mouse monoclonal (5C5)
  • immunocytochemistry; mouse; loading ...; fig 7e
  • western blot; mouse; loading ...; fig 5b
Sigma-Aldrich RWDD2A antibody (Sigma-Aldrich, A2172) was used in immunocytochemistry on mouse samples (fig 7e) and in western blot on mouse samples (fig 5b). Wound Repair Regen (2013) ncbi
Articles Reviewed
  1. Hogarth M, Houweling P, Thomas K, Gordish Dressman H, Bello L, Pegoraro E, et al. Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy. Nat Commun. 2017;8:14143 pubmed publisher
  2. van Moorsel D, Hansen J, Havekes B, Scheer F, Jorgensen J, Hoeks J, et al. Demonstration of a day-night rhythm in human skeletal muscle oxidative capacity. Mol Metab. 2016;5:635-645 pubmed publisher
  3. Kang C, Qiao Y, Li G, Baechle K, Camelliti P, Rentschler S, et al. Human Organotypic Cultured Cardiac Slices: New Platform For High Throughput Preclinical Human Trials. Sci Rep. 2016;6:28798 pubmed publisher
  4. SINGLA D, Wang J. Fibroblast Growth Factor-9 Activates c-Kit Progenitor Cells and Enhances Angiogenesis in the Infarcted Diabetic Heart. Oxid Med Cell Longev. 2016;2016:5810908 pubmed publisher
  5. Verbrugge S, Al M, Assaraf Y, Kammerer S, Chandrupatla D, Honeywell R, et al. Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR. Oncotarget. 2016;7:5240-57 pubmed publisher
  6. Yan G, Wang Q, Hu S, Wang D, Qiao Y, Ma G, et al. Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury. Int J Mol Med. 2015;36:1001-11 pubmed publisher
  7. Cardona M, López J, Serafín A, Rongvaux A, Inserte J, García Dorado D, et al. Executioner Caspase-3 and 7 Deficiency Reduces Myocyte Number in the Developing Mouse Heart. PLoS ONE. 2015;10:e0131411 pubmed publisher
  8. Kapoor N, Tran A, Kang J, Zhang R, Philipson K, Goldhaber J. Regulation of calcium clock-mediated pacemaking by inositol-1,4,5-trisphosphate receptors in mouse sinoatrial nodal cells. J Physiol. 2015;593:2649-63 pubmed publisher
  9. Hong Y, Frugier T, Zhang X, Murphy R, Lynch G, Betik A, et al. Glucose uptake during contraction in isolated skeletal muscles from neuronal nitric oxide synthase μ knockout mice. J Appl Physiol (1985). 2015;118:1113-21 pubmed publisher
  10. Swager S, Delfín D, Rastogi N, Wang H, Canan B, Fedorov V, et al. Claudin-5 levels are reduced from multiple cell types in human failing hearts and are associated with mislocalization of ephrin-B1. Cardiovasc Pathol. 2015;24:160-167 pubmed publisher
  11. Sin J, Puccini J, Huang C, Konstandin M, Gilbert P, Sussman M, et al. The impact of juvenile coxsackievirus infection on cardiac progenitor cells and postnatal heart development. PLoS Pathog. 2014;10:e1004249 pubmed publisher
  12. Deng Y, Xie D, Fang M, Zhu G, Chen C, Zeng H, et al. Astrocyte-derived proinflammatory cytokines induce hypomyelination in the periventricular white matter in the hypoxic neonatal brain. PLoS ONE. 2014;9:e87420 pubmed publisher
  13. Garton F, Seto J, Quinlan K, Yang N, Houweling P, North K. ?-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilization. Hum Mol Genet. 2014;23:1879-93 pubmed publisher
  14. Nworu C, Krieg P, Gregorio C. Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging. Methods. 2014;66:370-9 pubmed publisher
  15. Tomasek J, Haaksma C, Schwartz R, Howard E. Whole animal knockout of smooth muscle alpha-actin does not alter excisional wound healing or the fibroblast-to-myofibroblast transition. Wound Repair Regen. 2013;21:166-76 pubmed publisher