This is a Validated Antibody Database (VAD) review about human RUVBL1, based on 8 published articles (read how Labome selects the articles), using RUVBL1 antibody in all methods. It is aimed to help Labome visitors find the most suited RUVBL1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RUVBL1 synonym: ECP-54; ECP54; INO80H; NMP 238; NMP238; PONTIN; Pontin52; RVB1; TIH1; TIP49; TIP49A; ruvB-like 1; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; INO80 complex subunit H; RuvB (E coli homolog)-like 1; RuvB-like AAA ATPase; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIP60-associated protein 54-alpha; nuclear matrix protein 238; pontin 52

Knockout validation
Sigma-Aldrich
mouse monoclonal (5G3-11)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • RNA immunoprecipitation; mouse; fig 7
  • chromatin immunoprecipitation; mouse; fig 2
  • immunoprecipitation; mouse; fig s3
Sigma-Aldrich RUVBL1 antibody (sigma, SAB4200194) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1), in RNA immunoprecipitation on mouse samples (fig 7), in chromatin immunoprecipitation on mouse samples (fig 2) and in immunoprecipitation on mouse samples (fig s3). Nat Commun (2015) ncbi
Abcam
rabbit monoclonal
  • western blot; human; loading ...; fig 6g
Abcam RUVBL1 antibody (Abcam, ab133513) was used in western blot on human samples (fig 6g). Br J Cancer (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2943C1a)
  • western blot; mouse; fig 4
Santa Cruz Biotechnology RUVBL1 antibody (Santa Cruz, 81360) was used in western blot on mouse samples (fig 4). Nat Commun (2015) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; fig 4
Proteintech Group RUVBL1 antibody (Proteintech, 10210-2-AP) was used in western blot on human samples (fig 4). Sci Rep (2016) ncbi
Abnova
mouse monoclonal (3G4-1F8)
  • immunoprecipitation; human; loading ...; fig 3
  • immunocytochemistry; human; loading ...; fig 2
  • western blot; human; loading ...; fig 1
Abnova RUVBL1 antibody (Abnova, H00008607-M01) was used in immunoprecipitation on human samples (fig 3), in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 1). Int J Oncol (2014) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; loading ...; fig 4b
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, Invitrogen RUVBL1 antibody (Thermo Scientific, PA5-29278) was used in western blot on human samples (fig 4b). Mol Cell (2017) ncbi
Sigma-Aldrich
mouse monoclonal (5G3-11)
  • western blot; human; loading ...; fig 4b
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, Sigma-Aldrich RUVBL1 antibody (Sigma, SAB4200194) was used in western blot on human samples (fig 4b). Mol Cell (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 4a
In order to investigate how FYCO1 contributes to the integrity of the chromatoid body, Sigma-Aldrich RUVBL1 antibody (Sigma-Aldrich, HPA019948) was used in western blot on mouse samples (fig 4a). Autophagy (2017) ncbi
mouse monoclonal (5G3-11)
  • immunoprecipitation; human; fig s4b
Sigma-Aldrich RUVBL1 antibody (Sigma-Aldrich, SAB4200194) was used in immunoprecipitation on human samples (fig s4b). Mol Cell Biol (2015) ncbi
mouse monoclonal (5G3-11)
  • western blot; mouse; 1:500; fig 6
Sigma-Aldrich RUVBL1 antibody (Sigma, SAB4200194) was used in western blot on mouse samples at 1:500 (fig 6). Nat Commun (2015) ncbi
mouse monoclonal (5G3-11)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • RNA immunoprecipitation; mouse; fig 7
  • chromatin immunoprecipitation; mouse; fig 2
  • immunoprecipitation; mouse; fig s3
Sigma-Aldrich RUVBL1 antibody (sigma, SAB4200194) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1), in RNA immunoprecipitation on mouse samples (fig 7), in chromatin immunoprecipitation on mouse samples (fig 2) and in immunoprecipitation on mouse samples (fig s3). Nat Commun (2015) ncbi
Articles Reviewed
  1. McClurg U, Nabbi A, Ricordel C, Korolchuk S, McCracken S, Heer R, et al. Human ex vivo prostate tissue model system identifies ING3 as an oncoprotein. Br J Cancer. 2018;118:713-726 pubmed publisher
  2. Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  3. Da Ros M, Lehtiniemi T, Olotu O, Fischer D, Zhang F, Vihinen H, et al. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy. 2017;13:302-321 pubmed publisher
  4. Hintermair C, Voß K, Forné I, Heidemann M, Flatley A, Kremmer E, et al. Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression. Sci Rep. 2016;6:27401 pubmed publisher
  5. Mir R, Bele A, Mirza S, Srivastava S, Olou A, Ammons S, et al. A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression. Mol Cell Biol. 2015;36:886-99 pubmed publisher
  6. Tarangelo A, Lo N, Teng R, Kim E, Le L, Watson D, et al. Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression. Nat Commun. 2015;6:10028 pubmed publisher
  7. Boo K, Bhin J, Jeon Y, Kim J, Shin H, Park J, et al. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells. Nat Commun. 2015;6:6810 pubmed publisher
  8. Taniuchi K, Furihata M, Iwasaki S, Tanaka K, Shimizu T, Saito M, et al. RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion. Int J Oncol. 2014;44:1945-54 pubmed publisher