This is a Validated Antibody Database (VAD) review about human RUBCN, based on 9 published articles (read how Labome selects the articles), using RUBCN antibody in all methods. It is aimed to help Labome visitors find the most suited RUBCN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RUBCN synonym: KIAA0226; RUBICON; SCAR15

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D9F7)
  • western blot; human; 1:1000; loading ...; fig 5f
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s1e
Cell Signaling Technology RUBCN antibody (CST, 8465) was used in western blot on human samples at 1:1000 (fig 5f) and in western blot knockout validation on mouse samples at 1:1000 (fig s1e). PLoS Genet (2021) ncbi
MBL International
mouse (1H6)
  • western blot knockout validation; mouse; loading ...; fig 1c
MBL International RUBCN antibody (MBL international, M170-3) was used in western blot knockout validation on mouse samples (fig 1c). elife (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D9F7)
  • western blot; human; 1:1000; loading ...; fig 5f
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s1e
Cell Signaling Technology RUBCN antibody (CST, 8465) was used in western blot on human samples at 1:1000 (fig 5f) and in western blot knockout validation on mouse samples at 1:1000 (fig s1e). PLoS Genet (2021) ncbi
domestic rabbit monoclonal (D9F7)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Cell Signaling Technology RUBCN antibody (CST, 8465) was used in western blot on mouse samples at 1:1000 (fig 4b). Nat Commun (2020) ncbi
domestic rabbit monoclonal (D9F7)
  • western blot; human; 1:1000; loading ...; fig s3k
Cell Signaling Technology RUBCN antibody (Cell Signaling Technology, D9F7) was used in western blot on human samples at 1:1000 (fig s3k). Nature (2020) ncbi
domestic rabbit monoclonal (D8B2)
  • immunoprecipitation; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4b
Cell Signaling Technology RUBCN antibody (Cell Signaling, 7151-S) was used in immunoprecipitation on human samples (fig 4b) and in western blot on human samples (fig 4b). Am J Physiol Cell Physiol (2019) ncbi
domestic rabbit monoclonal (D9F7)
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology RUBCN antibody (Cell Signaling Technology, 8465) was used in western blot on mouse samples (fig 5a). EMBO J (2018) ncbi
domestic rabbit monoclonal (D9F7)
  • western blot; rat; loading ...; fig 2h
Cell Signaling Technology RUBCN antibody (Cell Signaling, 8465) was used in western blot on rat samples (fig 2h). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (D9F7)
  • western blot; mouse; 1:1000; fig 3
Cell Signaling Technology RUBCN antibody (Cell Signaling, 8465) was used in western blot on mouse samples at 1:1000 (fig 3). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D9F7)
  • western blot; human; fig 5g
Cell Signaling Technology RUBCN antibody (Cell Signaling Technologies., 8465) was used in western blot on human samples (fig 5g). EMBO J (2015) ncbi
MBL International
mouse (1H6)
  • western blot knockout validation; mouse; loading ...; fig 1c
MBL International RUBCN antibody (MBL international, M170-3) was used in western blot knockout validation on mouse samples (fig 1c). elife (2019) ncbi
Articles Reviewed
  1. Yamamuro T, Nakamura S, Yamano Y, Endo T, Yanagawa K, Tokumura A, et al. Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function. PLoS Genet. 2021;17:e1009688 pubmed publisher
  2. Yamamuro T, Kawabata T, Fukuhara A, Saita S, Nakamura S, Takeshita H, et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 2020;11:4150 pubmed publisher
  3. Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
  4. Lim J, Park H, Heisler J, Maculins T, Roose Girma M, Xu M, et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. elife. 2019;8: pubmed publisher
  5. Kumar V, Ayasolla K, Jha A, Mishra A, Vashistha H, Lan X, et al. Disrupted APOL1-miR193a Axis Dedifferentiates Podocytes through Autophagy Blockade in APOL1 Risk Milieu. Am J Physiol Cell Physiol. 2019;: pubmed publisher
  6. Hartlova A, Herbst S, Peltier J, Rodgers A, Bilkei Gorzo O, Fearns A, et al. LRRK2 is a negative regulator of Mycobacterium tuberculosis phagosome maturation in macrophages. EMBO J. 2018;37: pubmed publisher
  7. Bartolomeo R, Cinque L, De Leonibus C, Forrester A, Salzano A, Monfregola J, et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017;127:3717-3729 pubmed publisher
  8. Nemazanyy I, Montagnac G, Russell R, Morzyglod L, Burnol A, Guan K, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015;6:8283 pubmed publisher
  9. Munson M, Allen G, Toth R, Campbell D, Lucocq J, Ganley I. mTOR activates the VPS34-UVRAG complex to regulate autolysosomal tubulation and cell survival. EMBO J. 2015;34:2272-90 pubmed publisher