This is a Validated Antibody Database (VAD) review about human RTF1, based on 4 published articles (read how Labome selects the articles), using RTF1 antibody in all methods. It is aimed to help Labome visitors find the most suited RTF1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RTF1 synonym: GTL7; KIAA0252; RNA polymerase-associated protein RTF1 homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog; ortholog of mouse gene trap locus 7

Bethyl
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3e
Bethyl RTF1 antibody (Bethyl Laboratories, A300-179A) was used in western blot on mouse samples at 1:1000 (fig 3e). Nat Cell Biol (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl RTF1 antibody (Bethyl, A300-178A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; fig 2
  • western blot; mouse; fig 1a
Bethyl RTF1 antibody (Bethyl, A300-179A) was used in ChIP-Seq on mouse samples (fig 2) and in western blot on mouse samples (fig 1a). PLoS Genet (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 7
  • immunocytochemistry; human; fig 1a
Bethyl RTF1 antibody (Bethyl, A300-179A) was used in chromatin immunoprecipitation on human samples (fig 7) and in immunocytochemistry on human samples (fig 1a). Mol Cell Biol (2015) ncbi
Articles Reviewed
  1. Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto A, Yang Y, Ntziachristos P, et al. Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nat Cell Biol. 2016;18:1127-1138 pubmed publisher
  2. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  3. Yang Y, Li W, Hoque M, Hou L, Shen S, Tian B, et al. PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation. PLoS Genet. 2016;12:e1005794 pubmed publisher
  4. Cao Q, Yamamoto J, Isobe T, Tateno S, Murase Y, Chen Y, et al. Characterization of the Human Transcription Elongation Factor Rtf1: Evidence for Nonoverlapping Functions of Rtf1 and the Paf1 Complex. Mol Cell Biol. 2015;35:3459-70 pubmed publisher