This is a Validated Antibody Database (VAD) review about human RPE65, based on 30 published articles (read how Labome selects the articles), using RPE65 antibody in all methods. It is aimed to help Labome visitors find the most suited RPE65 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RPE65 synonym: BCO3; LCA2; RP20; mRPE65; p63; rd12; sRPE65

Santa Cruz Biotechnology
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2c
Santa Cruz Biotechnology RPE65 antibody (Santa Cruz Biotechnology, sc-8431) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 2c). Sci Adv (2019) ncbi
  • western blot; mouse; loading ...; fig 5d
Santa Cruz Biotechnology RPE65 antibody (Sana Cruz Biotechnology, Inc, sc-8431) was used in western blot on mouse samples (fig 5d). Carcinogenesis (2018) ncbi
  • immunohistochemistry; mouse; loading ...; fig 2e
Santa Cruz Biotechnology RPE65 antibody (Santa Cruz Biotechnology Inc, sc-8431) was used in immunohistochemistry on mouse samples (fig 2e). J Dent Res (2017) ncbi
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1a
Santa Cruz Biotechnology RPE65 antibody (Santa Cruz, sc-8431) was used in immunohistochemistry on mouse samples at 1:200 (fig 1a). J Clin Invest (2017) ncbi
  • immunohistochemistry; human; 1:100; loading ...; fig 5i
Santa Cruz Biotechnology RPE65 antibody (Santa Cruz, sc-8431) was used in immunohistochemistry on human samples at 1:100 (fig 5i). PLoS ONE (2017) ncbi
  • immunohistochemistry - frozen section; human; fig 1e
In order to maintain primary human epithelial cell sheets cultured without dermal equivalents for at least 1 year, Santa Cruz Biotechnology RPE65 antibody (Santa Cruz, sc-8431) was used in immunohistochemistry - frozen section on human samples (fig 1e). Sci Rep (2017) ncbi
mouse monoclonal (3D9)
  • immunohistochemistry - paraffin section; mouse; fig 3a
In order to evaluate the efficacy of systemically administered RPE65-programmed bone marrow-derived cells to prevent visual loss in a mouse model of age-related macular degeneration, Santa Cruz Biotechnology RPE65 antibody (Santa Cruz, sc-73616) was used in immunohistochemistry - paraffin section on mouse samples (fig 3a). Mol Ther (2017) ncbi
  • immunocytochemistry; mouse; 1:100; fig 4a
In order to design a model of mouse middle ear epithelial cells that recapitulates the characteristics of the native murine middle ear epithelium, Santa Cruz Biotechnology RPE65 antibody (Santa Cruz Biotechnology, Sc-8431) was used in immunocytochemistry on mouse samples at 1:100 (fig 4a). Dis Model Mech (2016) ncbi
  • immunoprecipitation; human; loading ...; fig 1d
  • western blot; human; 1:200; loading ...; fig 3e
Santa Cruz Biotechnology RPE65 antibody (Santa Cruz, sc-8431) was used in immunoprecipitation on human samples (fig 1d) and in western blot on human samples at 1:200 (fig 3e). Oncotarget (2016) ncbi
  • western blot; human; 1:500; loading ...; fig 2b
In order to propose that the NKX3.1-G9a-UTY transcriptional regulatory network is essential for prostate differentiation, Santa Cruz Biotechnology RPE65 antibody (Santa Cruz Biotechnology, sc-8431) was used in western blot on human samples at 1:500 (fig 2b). Science (2016) ncbi
  • flow cytometry; mouse; 1:100; fig 1
Santa Cruz Biotechnology RPE65 antibody (Santa Cruz, sc8431) was used in flow cytometry on mouse samples at 1:100 (fig 1). Prostate (2015) ncbi
mouse monoclonal (8B11)
  • immunohistochemistry - paraffin section; mouse; 1:1000
In order to determine the role of Notch signaling in the development of the anterior pigmented epithelium, Santa Cruz Biotechnology RPE65 antibody (Santa Cruz, sc-53489) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000. Pigment Cell Melanoma Res (2014) ncbi
Abcam
mouse monoclonal (401.8B11.3D9)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5a
Abcam RPE65 antibody (Abcam, ab13826) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 5a). Sci Rep (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 3d
  • western blot; human; loading ...; fig 3a
In order to characterize the differentiation of RPE cells, Abcam RPE65 antibody (Abcam, ab67042) was used in immunocytochemistry on human samples (fig 3d) and in western blot on human samples (fig 3a). Stem Cell Res Ther (2017) ncbi
mouse monoclonal (401.8B11.3D9)
  • western blot; mouse; loading ...; fig 4b
In order to assess the effects of N-retinyl-N-retinylidene ethanolamine on mitogen-activated protein kinase, Abcam RPE65 antibody (Abcam, ab13826) was used in western blot on mouse samples (fig 4b). Front Aging Neurosci (2017) ncbi
  • western blot; human; 1:200; fig 3
In order to investigate p63 and SETDB1 expression in breast cancer cells, Abcam RPE65 antibody (Abcam, ab97865) was used in western blot on human samples at 1:200 (fig 3). Oncotarget (2016) ncbi
Novus Biologicals
mouse monoclonal (401.8B11.3D9)
  • western blot; mouse; 1:1000; loading ...; fig s8
Novus Biologicals RPE65 antibody (Novus, 401.8B11.3D9) was used in western blot on mouse samples at 1:1000 (fig s8). Proc Natl Acad Sci U S A (2020) ncbi
mouse monoclonal (401.8B11.3D9)
  • immunohistochemistry; human; 1:200; fig 4
In order to establish an application to generate retinal pigmented epithelium from induced pluripotent stem cells, Novus Biologicals RPE65 antibody (Novus, NB100-355) was used in immunohistochemistry on human samples at 1:200 (fig 4). PLoS ONE (2017) ncbi
mouse monoclonal (401.8B11.3D9)
  • immunocytochemistry; pigs ; 1:50; fig 3
In order to characterize coculture model of retinal pigment epithelium cells and porcine central neuroretina explants, Novus Biologicals RPE65 antibody (Novus Biologicals, NB100-355) was used in immunocytochemistry on pigs samples at 1:50 (fig 3). Mol Vis (2016) ncbi
mouse monoclonal (401.8B11.3D9)
  • immunohistochemistry - paraffin section; mouse
  • immunohistochemistry; mouse; 1:100
Novus Biologicals RPE65 antibody (Novus Biologicals, NB100-355) was used in immunohistochemistry - paraffin section on mouse samples and in immunohistochemistry on mouse samples at 1:100. Mol Vis (2014) ncbi
Active Motif
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 6
Active Motif RPE65 antibody (ActiveMotif, 39739) was used in chromatin immunoprecipitation on human samples (fig 6). Cell Cycle (2016) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; fig s8
Active Motif RPE65 antibody (ActiveMotif, 39739) was used in ChIP-Seq on human samples (fig s8). Nat Genet (2016) ncbi
Invitrogen
mouse monoclonal (401.8B11.3D9)
  • immunocytochemistry; human; 1:100
In order to examine the in vitro differentiation capacity of human bone marrow-derived stem cells to develop into retinal lineages, Invitrogen RPE65 antibody (ThermoScientific, MA1-16578) was used in immunocytochemistry on human samples at 1:100. Exp Cell Res (2015) ncbi
Biocare Medical
  • immunohistochemistry; human; 1:250; fig 4
Biocare Medical RPE65 antibody (Biocare, CM163A) was used in immunohistochemistry on human samples at 1:250 (fig 4). Nat Genet (2016) ncbi
  • immunohistochemistry - paraffin section; human; loading ...; fig 2b
Biocare Medical RPE65 antibody (Biocare, CM 163 B) was used in immunohistochemistry - paraffin section on human samples (fig 2b). Int J Clin Exp Pathol (2015) ncbi
  • immunocytochemistry; human; 1:80
Biocare Medical RPE65 antibody (Biocare Medical, CM163C) was used in immunocytochemistry on human samples at 1:80. Cytopathology (2015) ncbi
Leica Biosystems
monoclonal (7JUL)
  • immunocytochemistry; human; 1:10; loading ...; fig 2
In order to discuss estrogen receptor regulation and function in normal breast and breast cancer, Leica Biosystems RPE65 antibody (Novocastra, 7JUL) was used in immunocytochemistry on human samples at 1:10 (fig 2). Oncotarget (2017) ncbi
monoclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
In order to discuss the role of cancer stem cells in moderately differentiated oral tongue squamous cell carcinoma, Leica Biosystems RPE65 antibody (Leica, NCL-p63) was used in immunohistochemistry - paraffin section on human samples (fig 1a). J Clin Pathol (2016) ncbi
monoclonal (7JUL)
  • immunohistochemistry; human; 1:40
In order to describe the histological features canalicular adenoma, Leica Biosystems RPE65 antibody (Leica, 7jul) was used in immunohistochemistry on human samples at 1:40. Head Neck Pathol (2015) ncbi
monoclonal (7JUL)
  • immunohistochemistry - paraffin section; human
Leica Biosystems RPE65 antibody (Leica, 7JUL) was used in immunohistochemistry - paraffin section on human samples . Virchows Arch (2014) ncbi
monoclonal
  • immunohistochemistry; human
Leica Biosystems RPE65 antibody (Novocastra, NCL-p63) was used in immunohistochemistry on human samples . BMC Cancer (2014) ncbi
Articles Reviewed
  1. Wright C, Uehara H, Kim Y, Yasuma T, Yasuma R, Hirahara S, et al. Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A. 2020;117:2579-2587 pubmed publisher
  2. Findlay A, McKie L, Keighren M, Clementson Mobbs S, Sanchez Pulido L, Wells S, et al. Fam151b, the mouse homologue of C.elegans menorin gene, is essential for retinal function. Sci Rep. 2020;10:437 pubmed publisher
  3. Francis R, Guo H, Streutker C, Ahmed M, Yung T, Dirks P, et al. Gastrointestinal transcription factors drive lineage-specific developmental programs in organ specification and cancer. Sci Adv. 2019;5:eaax8898 pubmed publisher
  4. Chen L, Hayden M, Gilmore E, Alexander Savino C, Oleksyn D, Gillespie K, et al. PKK deletion in basal keratinocytes promotes tumorigenesis after chemical carcinogenesis. Carcinogenesis. 2018;39:418-428 pubmed publisher
  5. Hazim R, Karumbayaram S, Jiang M, Dimashkie A, Lopes V, Li D, et al. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2017;8:217 pubmed publisher
  6. Kousa Y, Roushangar R, Patel N, Walter A, Marangoni P, Krumlauf R, et al. IRF6 and SPRY4 Signaling Interact in Periderm Development. J Dent Res. 2017;96:1306-1313 pubmed publisher
  7. Giroux V, Lento A, Islam M, Pitarresi J, Kharbanda A, Hamilton K, et al. Long-lived keratin 15+ esophageal progenitor cells contribute to homeostasis and regeneration. J Clin Invest. 2017;127:2378-2391 pubmed publisher
  8. Ojeh N, Akgul B, Tomic Canic M, Philpott M, Navsaria H. In vitro skin models to study epithelial regeneration from the hair follicle. PLoS ONE. 2017;12:e0174389 pubmed publisher
  9. Balmer D, Bapst Wicht L, Pyakurel A, Emery M, Nanchen N, Bochet C, et al. Bis-Retinoid A2E Induces an Increase of Basic Fibroblast Growth Factor via Inhibition of Extracellular Signal-Regulated Kinases 1/2 Pathway in Retinal Pigment Epithelium Cells and Facilitates Phagocytosis. Front Aging Neurosci. 2017;9:43 pubmed publisher
  10. Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher
  11. Miyashita H, Niwano H, Yoshida S, Hatou S, Inagaki E, Tsubota K, et al. Long-term homeostasis and wound healing in an in vitro epithelial stem cell niche model. Sci Rep. 2017;7:43557 pubmed publisher
  12. Qi X, Pay S, Yan Y, Thomas J, Lewin A, Chang L, et al. Systemic Injection of RPE65-Programmed Bone Marrow-Derived Cells Prevents Progression of Chronic Retinal Degeneration. Mol Ther. 2017;25:917-927 pubmed publisher
  13. Hopkinson B, Klitgaard M, Petersen O, Villadsen R, Rønnov Jessen L, Kim J. Establishment of a normal-derived estrogen receptor-positive cell line comparable to the prevailing human breast cancer subtype. Oncotarget. 2017;8:10580-10593 pubmed publisher
  14. Mulay A, Akram K, Williams D, Armes H, Russell C, Hood D, et al. An in vitro model of murine middle ear epithelium. Dis Model Mech. 2016;9:1405-1417 pubmed
  15. Han A, Li J, Li Y, Wang Y, Bergholz J, Zhang Y, et al. p63α modulates c-Myc activity via direct interaction and regulation of MM1 protein stability. Oncotarget. 2016;7:44277-44287 pubmed publisher
  16. Dutta A, Le Magnen C, Mitrofanova A, Ouyang X, Califano A, Abate Shen C. Identification of an NKX3.1-G9a-UTY transcriptional regulatory network that controls prostate differentiation. Science. 2016;352:1576-80 pubmed publisher
  17. Baillie R, Itinteang T, Yu H, Brasch H, Davis P, Tan S. Cancer stem cells in moderately differentiated oral tongue squamous cell carcinoma. J Clin Pathol. 2016;69:742-4 pubmed publisher
  18. Di Lauro S, Rodriguez Crespo D, Gayoso M, Garcia Gutierrez M, Pastor J, Srivastava G, et al. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Mol Vis. 2016;22:243-53 pubmed
  19. Katoh I, Fukunishi N, Fujimuro M, Kasai H, Moriishi K, Hata R, et al. Repression of Wnt/β-catenin response elements by p63 (TP63). Cell Cycle. 2016;15:699-710 pubmed publisher
  20. Regina C, Compagnone M, Peschiaroli A, Lena A, Annicchiarico Petruzzelli M, Piro M, et al. Setdb1, a novel interactor of ΔNp63, is involved in breast tumorigenesis. Oncotarget. 2016;7:28836-48 pubmed publisher
  21. Drier Y, Cotton M, Williamson K, Gillespie S, Ryan R, Kluk M, et al. An oncogenic MYB feedback loop drives alternate cell fates in adenoid cystic carcinoma. Nat Genet. 2016;48:265-72 pubmed publisher
  22. Abd Raboh N, Hakim S. Diagnostic role of DOG1 and p63 immunohistochemistry in salivary gland carcinomas. Int J Clin Exp Pathol. 2015;8:9214-22 pubmed
  23. Wang H, Wang L, Jerde T, Chan B, Savran C, Burcham G, et al. Characterization of autoimmune inflammation induced prostate stem cell expansion. Prostate. 2015;75:1620-31 pubmed publisher
  24. Mathivanan I, Trepp C, Brunold C, Baerlocher G, Enzmann V. Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro. Exp Cell Res. 2015;333:11-20 pubmed publisher
  25. Prasad T, Verma A, Li Q. Expression and cellular localization of the Mas receptor in the adult and developing mouse retina. Mol Vis. 2014;20:1443-55 pubmed
  26. Thompson L, Bauer J, Chiosea S, McHugh J, Seethala R, Miettinen M, et al. Canalicular adenoma: a clinicopathologic and immunohistochemical analysis of 67 cases with a review of the literature. Head Neck Pathol. 2015;9:181-95 pubmed publisher
  27. Jeon Y, Moon K, Park S, Chung D. Primary pulmonary myxoid sarcomas with EWSR1-CREB1 translocation might originate from primitive peribronchial mesenchymal cells undergoing (myo)fibroblastic differentiation. Virchows Arch. 2014;465:453-61 pubmed publisher
  28. L Abbate A, Lo Cunsolo C, Macri E, Iuzzolino P, Mecucci C, Doglioni C, et al. FOXP1 and TP63 involvement in the progression of myelodysplastic syndrome with 5q- and additional cytogenetic abnormalities. BMC Cancer. 2014;14:396 pubmed publisher
  29. Pinheiro C, Roque R, Adriano A, Mendes P, Praça M, Reis I, et al. Optimization of immunocytochemistry in cytology: comparison of two protocols for fixation and preservation on cytospin and smear preparations. Cytopathology. 2015;26:38-43 pubmed publisher
  30. Sarode B, Nowell C, Ihm J, Kostic C, Arsenijevic Y, Moulin A, et al. Notch signaling in the pigmented epithelium of the anterior eye segment promotes ciliary body development at the expense of iris formation. Pigment Cell Melanoma Res. 2014;27:580-9 pubmed publisher