This is a Validated Antibody Database (VAD) review about human RPA1, based on 20 published articles (read how Labome selects the articles), using RPA1 antibody in all methods. It is aimed to help Labome visitors find the most suited RPA1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RPA1 synonym: HSSB; MST075; REPA1; RF-A; RP-A; RPA70; replication protein A 70 kDa DNA-binding subunit; MSTP075; RF-A protein 1; RP-A p70; replication factor A protein 1; replication protein A1, 70kDa; single-stranded DNA-binding protein

Abcam
rabbit monoclonal (EPR3472)
  • immunocytochemistry; human; 1:1000; loading ...; fig 2c
Abcam RPA1 antibody (Abcam, ab79398) was used in immunocytochemistry on human samples at 1:1000 (fig 2c). Nat Cell Biol (2016) ncbi
rabbit monoclonal (EPR3472)
  • western blot; human; 1:1000; fig s7
Abcam RPA1 antibody (Abcam, ab79398) was used in western blot on human samples at 1:1000 (fig s7). Nat Commun (2016) ncbi
rabbit monoclonal (EPR3472)
  • western blot; human; fig 3
In order to investigate the role of topoisomerase IIbeta-binding protein 1 in DNA repair and its contribution to cancer, Abcam RPA1 antibody (Abcam, ab79398) was used in western blot on human samples (fig 3). J Cell Biol (2016) ncbi
rabbit monoclonal (EPR3472)
  • western blot; human; fig 7
In order to learn the association with S phase-specific defects in nucleotide excision repair and mutations in replicative stress response pathways, Abcam RPA1 antibody (Abcam, ab79398) was used in western blot on human samples (fig 7). J Biol Chem (2016) ncbi
mouse monoclonal (8C3-D12-H10)
  • western blot; rat; 1:80
Abcam RPA1 antibody (Abcam, ab176467) was used in western blot on rat samples at 1:80. Exp Cell Res (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-10)
  • western blot; mouse
Santa Cruz Biotechnology RPA1 antibody (Santa Cruz Biotechnology, sc-166023) was used in western blot on mouse samples . Nucleic Acids Res (2014) ncbi
mouse monoclonal (H-7)
  • western blot; mouse; 1:500
Santa Cruz Biotechnology RPA1 antibody (Santa Cruz, sc-48425) was used in western blot on mouse samples at 1:500. J Biol Chem (2012) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; mouse; fig 1
In order to study why HSC function declines with age, Invitrogen RPA1 antibody (Thermo Scientific, PA5-21976) was used in immunocytochemistry on mouse samples (fig 1). Nature (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 2d
Cell Signaling Technology RPA1 antibody (Cell Signaling, 2267) was used in immunocytochemistry on human samples (fig 2d). Cell Rep (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 6c
In order to study quinacrine-induced apoptosis, Cell Signaling Technology RPA1 antibody (Cell Signaling, 2267) was used in western blot on human samples (fig 6c). Biochem Pharmacol (2016) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology RPA1 antibody (Cell Signaling Technology, 2267) was used in western blot on human samples . DNA Repair (Amst) (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 3c
Cell Signaling Technology RPA1 antibody (Cell Signaling, 2267) was used in immunocytochemistry on human samples (fig 3c). Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 3
Cell Signaling Technology RPA1 antibody (Cell signaling, 2267) was used in immunocytochemistry on human samples (fig 3). Cancers (Basel) (2014) ncbi
EMD Millipore
mouse monoclonal
  • chromatin immunoprecipitation; human; loading ...; fig 4d
EMD Millipore RPA1 antibody (Millipore, NA-19) was used in chromatin immunoprecipitation on human samples (fig 4d). Nucleic Acids Res (2017) ncbi
mouse monoclonal
  • immunocytochemistry; human; 1:100; loading ...; fig st1
  • western blot; human; 1:1000; loading ...; fig st1
In order to analyze the effect of CX-5461 as a G-quadruplex DNA stabilizer, EMD Millipore RPA1 antibody (Calbiochem, NA19L) was used in immunocytochemistry on human samples at 1:100 (fig st1) and in western blot on human samples at 1:1000 (fig st1). Nat Commun (2017) ncbi
mouse monoclonal
  • dot blot; human; 1:500; loading ...; fig 2a
EMD Millipore RPA1 antibody (Calbiochem, NA19L) was used in dot blot on human samples at 1:500 (fig 2a). PLoS ONE (2016) ncbi
mouse monoclonal
  • western blot; human; 1:5000; loading ...; fig 4e
EMD Millipore RPA1 antibody (Calbiochem, NA19L) was used in western blot on human samples at 1:5000 (fig 4e). Nat Commun (2016) ncbi
mouse monoclonal
  • immunocytochemistry; mouse; 1:100; fig 1
EMD Millipore RPA1 antibody (Calbiochem, NA19L) was used in immunocytochemistry on mouse samples at 1:100 (fig 1). Nat Commun (2016) ncbi
mouse monoclonal
  • immunocytochemistry; human; 1:100; fig 1
  • western blot; human; 1:1000; fig 1
EMD Millipore RPA1 antibody (Calbiochem, NA13) was used in immunocytochemistry on human samples at 1:100 (fig 1) and in western blot on human samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (RPA9, 1-30)
  • western blot; human
EMD Millipore RPA1 antibody (Millipore, 04-1521) was used in western blot on human samples . J Biol Chem (2013) ncbi
Articles Reviewed
  1. Daddacha W, Koyen A, Bastien A, Head P, Dhere V, Nabeta G, et al. SAMHD1 Promotes DNA End Resection to Facilitate DNA Repair by Homologous Recombination. Cell Rep. 2017;20:1921-1935 pubmed publisher
  2. Feng X, Hsu S, Kasbek C, Chaiken M, Price C. CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance. Nucleic Acids Res. 2017;45:4281-4293 pubmed publisher
  3. Xu H, Di Antonio M, McKinney S, Mathew V, Ho B, O Neil N, et al. CX-5461 is a DNA G-quadruplex stabilizer with selective lethality in BRCA1/2 deficient tumours. Nat Commun. 2017;8:14432 pubmed publisher
  4. Li C, Miao Y, Lingeman R, Hickey R, Malkas L. Partial Purification of a Megadalton DNA Replication Complex by Free Flow Electrophoresis. PLoS ONE. 2016;11:e0169259 pubmed publisher
  5. Hansen R, Mund A, Poulsen S, Sandoval M, Klement K, Tsouroula K, et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol. 2016;18:1357-1366 pubmed publisher
  6. Despras E, Sittewelle M, Pouvelle C, Delrieu N, Cordonnier A, Kannouche P. Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA. Nat Commun. 2016;7:13326 pubmed publisher
  7. Passerini V, Ozeri Galai E, de Pagter M, Donnelly N, Schmalbrock S, Kloosterman W, et al. The presence of extra chromosomes leads to genomic instability. Nat Commun. 2016;7:10754 pubmed publisher
  8. Ahuja A, Jodkowska K, Teloni F, Bizard A, Zellweger R, Herrador R, et al. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016;7:10660 pubmed publisher
  9. Preet R, Siddharth S, Satapathy S, Das S, Nayak A, Das D, et al. Chk1 inhibitor synergizes quinacrine mediated apoptosis in breast cancer cells by compromising the base excision repair cascade. Biochem Pharmacol. 2016;105:23-33 pubmed publisher
  10. Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
  11. Bélanger F, Angers J, Fortier Ã, Hammond Martel I, Costantino S, Drobetsky E, et al. Mutations in Replicative Stress Response Pathways Are Associated with S Phase-specific Defects in Nucleotide Excision Repair. J Biol Chem. 2016;291:522-37 pubmed publisher
  12. Lee K, Im J, Shibata E, Park J, Handa N, Kowalczykowski S, et al. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun. 2015;6:7744 pubmed publisher
  13. Ghospurkar P, Wilson T, Liu S, Herauf A, Steffes J, Mueller E, et al. Phosphorylation and cellular function of the human Rpa2 N-terminus in the budding yeast Saccharomyces cerevisiae. Exp Cell Res. 2015;331:183-99 pubmed publisher
  14. Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, et al. The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function. DNA Repair (Amst). 2015;25:72-83 pubmed publisher
  15. Witosch J, Wolf E, Mizuno N. Architecture and ssDNA interaction of the Timeless-Tipin-RPA complex. Nucleic Acids Res. 2014;42:12912-27 pubmed publisher
  16. Smith S, Petrova A, Madden M, Wang H, Pan Y, Warren M, et al. A gemcitabine sensitivity screen identifies a role for NEK9 in the replication stress response. Nucleic Acids Res. 2014;42:11517-27 pubmed publisher
  17. Flach J, Bakker S, Mohrin M, Conroy P, Pietras E, Reynaud D, et al. Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells. Nature. 2014;512:198-202 pubmed publisher
  18. Diaz J, Wang X, Tsang S, Jiao J, You J. Phosphorylation of large T antigen regulates merkel cell polyomavirus replication. Cancers (Basel). 2014;6:1464-86 pubmed publisher
  19. Wei Q, Li J, Liu T, Tong X, Ye X. Phosphorylation of minichromosome maintenance protein 7 (MCM7) by cyclin/cyclin-dependent kinase affects its function in cell cycle regulation. J Biol Chem. 2013;288:19715-25 pubmed publisher
  20. Gao Z, Cox J, Gilmore J, Ormsbee B, Mallanna S, Washburn M, et al. Determination of protein interactome of transcription factor Sox2 in embryonic stem cells engineered for inducible expression of four reprogramming factors. J Biol Chem. 2012;287:11384-97 pubmed publisher