This is a Validated Antibody Database (VAD) review about human ROBO3, based on 4 published articles (read how Labome selects the articles), using ROBO3 antibody in all methods. It is aimed to help Labome visitors find the most suited ROBO3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ROBO3 synonym: HGPPS; HGPPS1; HGPS; RBIG1; RIG1; roundabout homolog 3; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3; roundabout-like protein 3

R&D Systems
goat polyclonal
  • immunohistochemistry; mouse; 1:250; loading ...; fig 1a
In order to understand the contribution of floor-plate-derived netrin-1 to commissural axon guidance, R&D Systems ROBO3 antibody (R&D Systems, AF3076) was used in immunohistochemistry on mouse samples at 1:250 (fig 1a). Nature (2017) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:500; fig 4
In order to analyze secreted semaphorin sink to facilitate commissural axon midline crossing by floor plate-derived neuropilin-2, R&D Systems ROBO3 antibody (R&D Systems, AF3076) was used in immunohistochemistry on mouse samples at 1:500 (fig 4). Genes Dev (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100
R&D Systems ROBO3 antibody (R&D, AF3076) was used in immunohistochemistry - frozen section on mouse samples at 1:100. Nat Neurosci (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:20
R&D Systems ROBO3 antibody (R&D Systems, AF3076) was used in immunohistochemistry - frozen section on mouse samples at 1:20. J Comp Neurol (2012) ncbi
Articles Reviewed
  1. Dominici C, Moreno Bravo J, Puiggros S, Rappeneau Q, Rama N, Vieugué P, et al. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance. Nature. 2017;545:350-354 pubmed publisher
  2. Hernández Enríquez B, Wu Z, Martinez E, Olsen O, Kaprielian Z, Maness P, et al. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing. Genes Dev. 2015;29:2617-32 pubmed publisher
  3. Delloye Bourgeois C, Jacquier A, Charoy C, Reynaud F, Nawabi H, Thoinet K, et al. PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments. Nat Neurosci. 2015;18:36-45 pubmed publisher
  4. Hashimoto M, Ito R, Kitamura N, Namba K, Hisano Y. Epha4 controls the midline crossing and contralateral axonal projections of inferior olive neurons. J Comp Neurol. 2012;520:1702-20 pubmed publisher