This is a Validated Antibody Database (VAD) review about human RNF8, based on 10 published articles (read how Labome selects the articles), using RNF8 antibody in all methods. It is aimed to help Labome visitors find the most suited RNF8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RNF8 synonym: hRNF8; E3 ubiquitin-protein ligase RNF8; C3HC4-type zinc finger protein; RING-type E3 ubiquitin transferase RNF8; UBC13/UEV-interacting ring finger protein; ring finger protein (C3HC4 type) 8; ring finger protein 8, E3 ubiquitin protein ligase

Santa Cruz Biotechnology
mouse monoclonal (B-2)
  • western blot; human; loading ...; fig 6c
Santa Cruz Biotechnology RNF8 antibody (Santa Cruz Biotechnology, sc-271462) was used in western blot on human samples (fig 6c). J Clin Invest (2018) ncbi
mouse monoclonal (B-2)
  • western blot; human; 1:200; loading ...; fig s3a
In order to describe a role for NIPBL in DNA damage repair, Santa Cruz Biotechnology RNF8 antibody (Santa Cruz, SC-271462) was used in western blot on human samples at 1:200 (fig s3a). J Cell Sci (2017) ncbi
mouse monoclonal
  • western blot; human; 1:1000; loading ...; fig 3c
In order to find inhibitors of the RNF8-Ubc13 interaction, Santa Cruz Biotechnology RNF8 antibody (Santa Cruz, B-2) was used in western blot on human samples at 1:1000 (fig 3c). SLAS Discov (2017) ncbi
mouse monoclonal (B-2)
  • western blot; human; 1:1000; loading ...; fig 3c
In order to find inhibitors of the RNF8-Ubc13 interaction, Santa Cruz Biotechnology RNF8 antibody (Santa Cruz, B-2) was used in western blot on human samples at 1:1000 (fig 3c). SLAS Discov (2017) ncbi
mouse monoclonal (B-2)
  • immunohistochemistry; human; 1:40; fig 6
  • western blot; human; fig 2
Santa Cruz Biotechnology RNF8 antibody (santa Cruz, sc-271462) was used in immunohistochemistry on human samples at 1:40 (fig 6) and in western blot on human samples (fig 2). J Exp Clin Cancer Res (2016) ncbi
mouse monoclonal (B-2)
  • proximity ligation assay; human; fig 4
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 3
Santa Cruz Biotechnology RNF8 antibody (Santa Cruz, sc-271462) was used in proximity ligation assay on human samples (fig 4), in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 3). Nucleus (2015) ncbi
mouse monoclonal (B-2)
  • western blot; mouse; 1:1000; fig s4
Santa Cruz Biotechnology RNF8 antibody (Santa Cruz, B-2) was used in western blot on mouse samples at 1:1000 (fig s4). Nature (2015) ncbi
mouse monoclonal (B-2)
  • western blot; human
Santa Cruz Biotechnology RNF8 antibody (Santa, sc-271462) was used in western blot on human samples . Genes Dev (2014) ncbi
mouse monoclonal (B-2)
  • immunocytochemistry; human
  • western blot; human; fig 4
Santa Cruz Biotechnology RNF8 antibody (Santa Cruz Biotechnology, sc-271462) was used in immunocytochemistry on human samples and in western blot on human samples (fig 4). Nucleic Acids Res (2014) ncbi
mouse monoclonal (B-2)
  • immunocytochemistry; human
  • western blot; human
In order to study the mechanism through which the Bloom syndrome helicase suppresses homologous recombination at sites of DNA damage, Santa Cruz Biotechnology RNF8 antibody (Santa Cruz, sc-271462) was used in immunocytochemistry on human samples and in western blot on human samples . EMBO J (2013) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; loading ...; fig 1b
Proteintech Group RNF8 antibody (Proteintech, 14112-1-AP) was used in western blot on human samples (fig 1b). Nucleic Acids Res (2016) ncbi
Articles Reviewed
  1. Li L, Guturi K, Gautreau B, Patel P, Saad A, Morii M, et al. Ubiquitin ligase RNF8 suppresses Notch signaling to regulate mammary development and tumorigenesis. J Clin Invest. 2018;128:4525-4542 pubmed publisher
  2. Bot C, Pfeiffer A, Giordano F, Manjeera D, Dantuma N, Ström L. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J Cell Sci. 2017;130:1134-1146 pubmed publisher
  3. Weber E, Rothenaigner I, Brandner S, Hadian K, Schorpp K. A High-Throughput Screening Strategy for Development of RNF8-Ubc13 Protein-Protein Interaction Inhibitors. SLAS Discov. 2017;22:316-323 pubmed publisher
  4. Kuang J, Li L, Guo L, Su Y, Wang Y, Xu Y, et al. RNF8 promotes epithelial-mesenchymal transition of breast cancer cells. J Exp Clin Cancer Res. 2016;35:88 pubmed publisher
  5. Lyons S, Achorn C, Kedersha N, Anderson P, Ivanov P. YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression. Nucleic Acids Res. 2016;44:6949-60 pubmed publisher
  6. Rassoolzadeh H, Coucoravas C, Farnebo M. The proximity ligation assay reveals that at DNA double-strand breaks WRAP53β associates with γH2AX and controls interactions between RNF8 and MDC1. Nucleus. 2015;6:417-24 pubmed publisher
  7. Xu G, Chapman J, Brandsma I, Yuan J, Mistrik M, Bouwman P, et al. REV7 counteracts DNA double-strand break resection and affects PARP inhibition. Nature. 2015;521:541-544 pubmed publisher
  8. Henriksson S, Rassoolzadeh H, Hedström E, Coucoravas C, Julner A, Goldstein M, et al. The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. Genes Dev. 2014;28:2726-38 pubmed publisher
  9. Munoz M, Yanez D, Stark J. An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Nucleic Acids Res. 2014;42:7720-33 pubmed publisher
  10. Tikoo S, Madhavan V, Hussain M, Miller E, Arora P, Zlatanou A, et al. Ubiquitin-dependent recruitment of the Bloom syndrome helicase upon replication stress is required to suppress homologous recombination. EMBO J. 2013;32:1778-92 pubmed publisher