This is a Validated Antibody Database (VAD) review about human RNF20, based on 8 published articles (read how Labome selects the articles), using RNF20 antibody in all methods. It is aimed to help Labome visitors find the most suited RNF20 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RNF20 synonym: BRE1; BRE1A; hBRE1; E3 ubiquitin-protein ligase BRE1A; BRE1 E3 ubiquitin ligase homolog; RING-type E3 ubiquitin transferase BRE1A; homolog of S. cerevisiae BRE1

Abcam
rabbit polyclonal
  • western blot; human; 1:2000; fig s11
In order to report that the RAP80-binding partner TRAIP regulates recruitment of the damage signaling machinery and promotes homologous recombination, Abcam RNF20 antibody (Abcam, 33500) was used in western blot on human samples at 1:2000 (fig s11). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1b
Abcam RNF20 antibody (Abcam, ab32629) was used in western blot on mouse samples (fig 1b). EMBO J (2015) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Abcam RNF20 antibody (Abcam, ab32629) was used in western blot on mouse samples (fig 5). Biochim Biophys Acta (2015) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4c
Abcam RNF20 antibody (abcam, ab32629) was used in western blot on mouse samples (fig 4c). J Cell Biol (2014) ncbi
rabbit polyclonal
  • western blot; human; fig 3b
In order to study the role of USP15 in the mechanism by which SART3 modulates histone H2B deubiquitinylation, Abcam RNF20 antibody (Abcam, ab32629) was used in western blot on human samples (fig 3b). J Biol Chem (2014) ncbi
Novus Biologicals
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig s2d
  • western blot; human; 1:2000; loading ...; fig 1c
  • western blot; mouse; 1:2000; loading ...; fig 6a
Novus Biologicals RNF20 antibody (Novus, B100-2242) was used in immunoprecipitation on human samples (fig s2d), in western blot on human samples at 1:2000 (fig 1c) and in western blot on mouse samples at 1:2000 (fig 6a). Nucleic Acids Res (2017) ncbi
Invitrogen
rabbit polyclonal
  • immunoprecipitation; human; fig 3
In order to study the epigenetic mechanisms by which SUPT6H regulates estrogen receptor activity, Invitrogen RNF20 antibody (Thermo Scientific, PA5-19597) was used in immunoprecipitation on human samples (fig 3). Oncogene (2015) ncbi
Bethyl
rabbit polyclonal
  • western blot; mouse; fig 4b
Bethyl RNF20 antibody (Bethyl, A300-714A) was used in western blot on mouse samples (fig 4b). J Cell Biol (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D6E10)
  • immunoprecipitation; human; fig 2g
  • chromatin immunoprecipitation; mouse; fig 2i
Cell Signaling Technology RNF20 antibody (Cell Signaling, 11974) was used in immunoprecipitation on human samples (fig 2g) and in chromatin immunoprecipitation on mouse samples (fig 2i). EMBO J (2015) ncbi
rabbit monoclonal (D8C2)
  • western blot; human; fig 5
Cell Signaling Technology RNF20 antibody (Cell Signaling, 9425) was used in western blot on human samples (fig 5). Cell (2015) ncbi
Articles Reviewed
  1. Chen S, Jing Y, Kang X, Yang L, Wang D, Zhang W, et al. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy. Nucleic Acids Res. 2017;45:1144-1158 pubmed publisher
  2. Soo Lee N, Jin Chung H, Kim H, Yun Lee S, Ji J, Seo Y, et al. TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage. Nat Commun. 2016;7:10463 pubmed publisher
  3. Yao X, Tang Z, Fu X, Yin J, Liang Y, Li C, et al. The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. EMBO J. 2015;34:2885-902 pubmed publisher
  4. Chen F, Woodfin A, Gardini A, Rickels R, Marshall S, Smith E, et al. PAF1, a Molecular Regulator of Promoter-Proximal Pausing by RNA Polymerase II. Cell. 2015;162:1003-15 pubmed publisher
  5. Wijeweera A, Haj M, Feldman A, Pnueli L, Luo Z, Melamed P. Gonadotropin gene transcription is activated by menin-mediated effects on the chromatin. Biochim Biophys Acta. 2015;1849:328-41 pubmed publisher
  6. Klement K, Luijsterburg M, Pinder J, Cena C, Del Nero V, Wintersinger C, et al. Opposing ISWI- and CHD-class chromatin remodeling activities orchestrate heterochromatic DNA repair. J Cell Biol. 2014;207:717-33 pubmed publisher
  7. Long L, Thelen J, Furgason M, Haj Yahya M, Brik A, Cheng D, et al. The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem. 2014;289:8916-30 pubmed publisher
  8. Bedi U, Scheel A, Hennion M, Begus Nahrmann Y, Ruschoff J, Johnsen S. SUPT6H controls estrogen receptor activity and cellular differentiation by multiple epigenomic mechanisms. Oncogene. 2015;34:465-73 pubmed publisher