This is a Validated Antibody Database (VAD) review about human RNF168, based on 10 published articles (read how Labome selects the articles), using RNF168 antibody in all methods. It is aimed to help Labome visitors find the most suited RNF168 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RNF168 synonym: hRNF168; E3 ubiquitin-protein ligase RNF168; RING-type E3 ubiquitin transferase RNF168; ring finger protein 168, E3 ubiquitin protein ligase

Santa Cruz Biotechnology
mouse monoclonal (B-11)
  • immunocytochemistry; human
  • western blot; human
In order to study the mechanism through which the Bloom syndrome helicase suppresses homologous recombination at sites of DNA damage, Santa Cruz Biotechnology RNF168 antibody (Santa Cruz, sc-101125) was used in immunocytochemistry on human samples and in western blot on human samples . EMBO J (2013) ncbi
EMD Millipore
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig s3b
  • western blot; human; 1:1000; loading ...; fig s3a
In order to describe a role for NIPBL in DNA damage repair, EMD Millipore RNF168 antibody (Millipore, ABE367) was used in immunocytochemistry on human samples at 1:1000 (fig s3b) and in western blot on human samples at 1:1000 (fig s3a). J Cell Sci (2017) ncbi
rabbit polyclonal
  • western blot; human; fig EV4
EMD Millipore RNF168 antibody (Millipore, 06- 1130) was used in western blot on human samples (fig EV4). EMBO Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3f
EMD Millipore RNF168 antibody (Millipore, 06-1130) was used in western blot on mouse samples at 1:1000 (fig 3f). EMBO Mol Med (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3
EMD Millipore RNF168 antibody (Millipore, ABE367) was used in western blot on human samples (fig 3). Nucleus (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 4
EMD Millipore RNF168 antibody (Millipore, 06-1130) was used in immunocytochemistry on human samples (fig 4). Nature (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 4
EMD Millipore RNF168 antibody (Millipore, ABE367) was used in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 4). Cell Death Dis (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 5
EMD Millipore RNF168 antibody (Millipore, 06-1130) was used in western blot on human samples (fig 5). Nucleic Acids Res (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 2b
EMD Millipore RNF168 antibody (Millipore, ABE367) was used in immunocytochemistry on human samples (fig 2b). Genes Dev (2014) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore RNF168 antibody (Millipore, 06-1130) was used in western blot on human samples . Nucleic Acids Res (2014) ncbi
Articles Reviewed
  1. Bot C, Pfeiffer A, Giordano F, Manjeera D, Dantuma N, Ström L. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J Cell Sci. 2017;130:1134-1146 pubmed publisher
  2. Choe K, Nicolae C, Constantin D, Imamura Kawasawa Y, Delgado Diaz M, De S, et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. 2016;17:874-86 pubmed publisher
  3. Gruosso T, Mieulet V, Cardon M, Bourachot B, Kieffer Y, Devun F, et al. Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patients. EMBO Mol Med. 2016;8:527-49 pubmed publisher
  4. Rassoolzadeh H, Coucoravas C, Farnebo M. The proximity ligation assay reveals that at DNA double-strand breaks WRAP53β associates with γH2AX and controls interactions between RNF8 and MDC1. Nucleus. 2015;6:417-24 pubmed publisher
  5. Thorslund T, Ripplinger A, Hoffmann S, Wild T, Uckelmann M, Villumsen B, et al. Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage. Nature. 2015;527:389-93 pubmed publisher
  6. Hedström E, Pederiva C, Farnebo J, Nodin B, Jirström K, Brennan D, et al. Downregulation of the cancer susceptibility protein WRAP53β in epithelial ovarian cancer leads to defective DNA repair and poor clinical outcome. Cell Death Dis. 2015;6:e1892 pubmed publisher
  7. Peng B, Wang J, Hu Y, Zhao H, Hou W, Zhao H, et al. Modulation of LSD1 phosphorylation by CK2/WIP1 regulates RNF168-dependent 53BP1 recruitment in response to DNA damage. Nucleic Acids Res. 2015;43:5936-47 pubmed publisher
  8. Henriksson S, Rassoolzadeh H, Hedström E, Coucoravas C, Julner A, Goldstein M, et al. The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. Genes Dev. 2014;28:2726-38 pubmed publisher
  9. Munoz M, Yanez D, Stark J. An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Nucleic Acids Res. 2014;42:7720-33 pubmed publisher
  10. Tikoo S, Madhavan V, Hussain M, Miller E, Arora P, Zlatanou A, et al. Ubiquitin-dependent recruitment of the Bloom syndrome helicase upon replication stress is required to suppress homologous recombination. EMBO J. 2013;32:1778-92 pubmed publisher