This is a Validated Antibody Database (VAD) review about human RIF1, based on 9 published articles (read how Labome selects the articles), using RIF1 antibody in all methods. It is aimed to help Labome visitors find the most suited RIF1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RIF1 synonym: telomere-associated protein RIF1; RAP1 interacting factor homolog; rap1-interacting factor 1 homolog

Bethyl
rabbit polyclonal
  • immunocytochemistry; human; 1:500; loading ...; fig 4a
Bethyl RIF1 antibody (Bethyl, A300-568A) was used in immunocytochemistry on human samples at 1:500 (fig 4a). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 1e
  • western blot; human; loading ...; fig s1
Bethyl RIF1 antibody (Bethyl Laboratories, A300-569A) was used in immunocytochemistry on human samples (fig 1e) and in western blot on human samples (fig s1). Oncotarget (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 3
Bethyl RIF1 antibody (Bethyl Laboratories, A300-569A) was used in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 3). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4
Bethyl RIF1 antibody (Bethyl Laboratories, SC-8017) was used in western blot on human samples at 1:1000 (fig 4). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 1
In order to study how the Fanconi anemia pathway promotes homologous recombination at stalled replication forks, Bethyl RIF1 antibody (Bethyl, A300-569A) was used in immunocytochemistry on human samples (fig 1). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:500; fig 1
Bethyl RIF1 antibody (Bethyl Laboratories, A300-569A) was used in immunocytochemistry on human samples at 1:500 (fig 1). Cell Cycle (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:10,000; fig 1
Bethyl RIF1 antibody (Bethyl, A300-569A) was used in western blot on human samples at 1:10,000 (fig 1). Nat Commun (2014) ncbi
Abcam
rabbit polyclonal
  • immunoprecipitation; human; loading ...
In order to determine specific residues on four MCM helicase subunits that show hyperphosphorylation upon RIF1 depletion, Abcam RIF1 antibody (Abcam, ab70254) was used in immunoprecipitation on human samples . EMBO Rep (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:50; fig s2
In order to elucidate the spindle proteome that is clathirin-dependent, Abcam RIF1 antibody (Abcam, ab13422) was used in immunocytochemistry on human samples at 1:50 (fig s2). Mol Cell Proteomics (2016) ncbi
Articles Reviewed
  1. Hiraga S, Ly T, Garzón J, Horejsi Z, Ohkubo Y, Endo A, et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep. 2017;18:403-419 pubmed publisher
  2. Zhang H, Sun L, Wang K, Wu D, Trappio M, Witting C, et al. Loss of H3K9me3 Correlates with ATM Activation and Histone H2AX Phosphorylation Deficiencies in Hutchinson-Gilford Progeria Syndrome. PLoS ONE. 2016;11:e0167454 pubmed publisher
  3. Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, et al. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget. 2016;7:57679-57693 pubmed publisher
  4. Rao S, Flores Rodriguez N, Page S, Wong C, Robinson P, Chircop M. The Clathrin-dependent Spindle Proteome. Mol Cell Proteomics. 2016;15:2537-53 pubmed publisher
  5. Chiang T, le Sage C, Larrieu D, Demir M, Jackson S. CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016;6:24356 pubmed publisher
  6. Zhang H, Liu H, Chen Y, Yang X, Wang P, Liu T, et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016;7:10201 pubmed publisher
  7. Renaud E, Barascu A, Rosselli F. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res. 2016;44:648-56 pubmed publisher
  8. Kocyłowski M, Rey A, Stewart G, Halazonetis T. Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168. Cell Cycle. 2015;14:1748-58 pubmed publisher
  9. Fernandez Vidal A, Guitton Sert L, Cadoret J, Drac M, Schwob E, Baldacci G, et al. A role for DNA polymerase ? in the timing of DNA replication. Nat Commun. 2014;5:4285 pubmed publisher