This is a Validated Antibody Database (VAD) review about human RBPJ, based on 19 published articles (read how Labome selects the articles), using RBPJ antibody in all methods. It is aimed to help Labome visitors find the most suited RBPJ antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RBPJ synonym: AOS3; CBF1; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH; csl

Abcam
domestic rabbit polyclonal
  • western blot; human; 1:2000; loading ...; fig 5c
Abcam RBPJ antibody (Abcam, ab25949) was used in western blot on human samples at 1:2000 (fig 5c). Genome Biol (2020) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4d
  • western blot; human; fig 7e
Abcam RBPJ antibody (Abcam, AB25949) was used in chromatin immunoprecipitation on human samples (fig 4d) and in western blot on human samples (fig 7e). J Virol (2017) ncbi
domestic rabbit monoclonal (EPR13479)
  • immunocytochemistry; rat; 1:100; loading ...; fig 5d
  • immunohistochemistry; rat; 1:100; loading ...; fig 2d
  • western blot; rat; 1:500; loading ...; fig 2a
In order to determine if hypertonic saline induces Notch signaling to regulate the production of proinflammatory mediators, Abcam RBPJ antibody (Abcam, ab180588) was used in immunocytochemistry on rat samples at 1:100 (fig 5d), in immunohistochemistry on rat samples at 1:100 (fig 2d) and in western blot on rat samples at 1:500 (fig 2a). BMC Neurosci (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 5a
Abcam RBPJ antibody (abcam, ab25949) was used in chromatin immunoprecipitation on human samples (fig 5a). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; fig 3
  • western blot; human
Abcam RBPJ antibody (Abcam, AB25949) was used in ChIP-Seq on human samples (fig 3) and in western blot on human samples . PLoS Pathog (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; mouse; 1:50; fig 6d
Cell Signaling Technology RBPJ antibody (CST, 5313) was used in chromatin immunoprecipitation on mouse samples at 1:50 (fig 6d). PLoS Genet (2021) ncbi
domestic rabbit monoclonal (D10A4)
  • immunohistochemistry - frozen section; rat; loading ...; fig 4b
  • western blot; rat; loading ...; fig 4d
Cell Signaling Technology RBPJ antibody (Cell Signaling Technology, 5313) was used in immunohistochemistry - frozen section on rat samples (fig 4b) and in western blot on rat samples (fig 4d). Sci Rep (2020) ncbi
domestic rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; human; fig 3d
Cell Signaling Technology RBPJ antibody (Cell Signaling Technology, 5313) was used in chromatin immunoprecipitation on human samples (fig 3d). elife (2020) ncbi
domestic rabbit monoclonal (D10A4)
  • western blot; human; loading ...; fig 6b
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5313) was used in western blot on human samples (fig 6b). J Virol (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5442S) was used in immunohistochemistry - frozen section on mouse samples (fig 1a). J Clin Invest (2019) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig s5a
  • western blot; human; loading ...; fig s5a
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5442) was used in immunoprecipitation on human samples (fig s5a) and in western blot on human samples (fig s5a). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (D10A4)
  • immunohistochemistry; mouse; loading ...; fig 2t
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5313) was used in immunohistochemistry on mouse samples (fig 2t). Sci Rep (2017) ncbi
domestic rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; human; loading ...; fig 5a
Cell Signaling Technology RBPJ antibody (Cell signaling, 5313s) was used in chromatin immunoprecipitation on human samples (fig 5a). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D10A4)
  • western blot; human; 1:800; loading ...; fig 3a
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5313) was used in western blot on human samples at 1:800 (fig 3a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; mouse; fig 7d
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5313) was used in chromatin immunoprecipitation on mouse samples (fig 7d). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D10A4)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6b
  • western blot; mouse; loading ...; fig s1
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5313) was used in immunohistochemistry - paraffin section on mouse samples (fig 6b) and in western blot on mouse samples (fig s1). J Clin Invest (2016) ncbi
domestic rabbit monoclonal (D10A4)
  • western blot; mouse; 1:1000; fig 2
In order to define transcriptional, metabolic ,and ubiquitin-cycling nodes controlled by Rbpj, Pfkl and Rnf145 during the phagocyte oxidative burst, Cell Signaling Technology RBPJ antibody (Cell Signalling Technology, 5313S) was used in western blot on mouse samples at 1:1000 (fig 2). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D10A4)
  • immunohistochemistry; mouse; 1:500
  • western blot; mouse; 1:1000
In order to study the role of Notch-dependent erroneous cell cycle reentry in neurodegeneration, Cell Signaling Technology RBPJ antibody (Cell Signaling, 5313) was used in immunohistochemistry on mouse samples at 1:500 and in western blot on mouse samples at 1:1000. Cell Death Differ (2015) ncbi
domestic rabbit monoclonal (D10A4)
  • chromatin immunoprecipitation; human
Cell Signaling Technology RBPJ antibody (Cell Signaling, 5313) was used in chromatin immunoprecipitation on human samples . PLoS ONE (2013) ncbi
InvivoGen
  • ELISA; human
InvivoGen RBPJ antibody (Invivogen, hrp-igak) was used in ELISA on human samples . Biotechnol Bioeng (2015) ncbi
Articles Reviewed
  1. Bao H, Sun Y, Yang N, Deng N, Ni Z, Tang Y, et al. Uterine Notch2 facilitates pregnancy recognition and corpus luteum maintenance via upregulating decidual Prl8a2. PLoS Genet. 2021;17:e1009786 pubmed publisher
  2. Xu W, Xu Y, Zhang X, Xu Y, Jian X, Chen J, et al. Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis. Sci Rep. 2020;10:17486 pubmed publisher
  3. Pan L, Lemieux M, Thomas T, Rogers J, Lipper C, Lee W, et al. IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation. elife. 2020;9: pubmed publisher
  4. Eyler C, Matsunaga H, Hovestadt V, Vantine S, van Galen P, Bernstein B. Single-cell lineage analysis reveals genetic and epigenetic interplay in glioblastoma drug resistance. Genome Biol. 2020;21:174 pubmed publisher
  5. Papp B, Motlagh N, Smindak R, Jin Jang S, Sharma A, Alonso J, et al. Genome-Wide Identification of Direct RTA Targets Reveals Key Host Factors for Kaposi's Sarcoma-Associated Herpesvirus Lytic Reactivation. J Virol. 2019;93: pubmed publisher
  6. Bartolomé A, Zhu C, Sussel L, Pajvani U. Notch signaling dynamically regulates adult β cell proliferation and maturity. J Clin Invest. 2019;129:268-280 pubmed publisher
  7. Li L, Guturi K, Gautreau B, Patel P, Saad A, Morii M, et al. Ubiquitin ligase RNF8 suppresses Notch signaling to regulate mammary development and tumorigenesis. J Clin Invest. 2018;128:4525-4542 pubmed publisher
  8. Lu F, Wiedmer A, Martin K, Wickramasinghe P, Kossenkov A, Lieberman P. Coordinate Regulation of TET2 and EBNA2 Controls the DNA Methylation State of Latent Epstein-Barr Virus. J Virol. 2017;91: pubmed publisher
  9. Watanabe Y, Miyasaka K, Kubo A, Kida Y, Nakagawa O, Hirate Y, et al. Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm. Sci Rep. 2017;7:46135 pubmed publisher
  10. Zeng W, Han Y, Zhu G, Huang L, Deng Y, Wang Q, et al. Hypertonic saline attenuates expression of Notch signaling and proinflammatory mediators in activated microglia in experimentally induced cerebral ischemia and hypoxic BV-2 microglia. BMC Neurosci. 2017;18:32 pubmed publisher
  11. Mouillesseaux K, Wiley D, Saunders L, Wylie L, Kushner E, Chong D, et al. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Nat Commun. 2016;7:13247 pubmed publisher
  12. Asnaghi L, Tripathy A, Yang Q, Kaur H, Hanaford A, Yu W, et al. Targeting Notch signaling as a novel therapy for retinoblastoma. Oncotarget. 2016;7:70028-70044 pubmed publisher
  13. Klein Hessling S, Rudolf R, Muhammad K, Knobeloch K, Maqbool M, Cauchy P, et al. A threshold level of NFATc1 activity facilitates thymocyte differentiation and opposes notch-driven leukaemia development. Nat Commun. 2016;7:11841 pubmed publisher
  14. Wang C, Inzana J, Mirando A, Ren Y, Liu Z, Shen J, et al. NOTCH signaling in skeletal progenitors is critical for fracture repair. J Clin Invest. 2016;126:1471-81 pubmed publisher
  15. Lu F, Chen H, Kossenkov A, DeWispeleare K, Won K, Lieberman P. EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-jκ and EBF1. PLoS Pathog. 2016;12:e1005339 pubmed publisher
  16. Graham D, Becker C, Doan A, Goel G, Villablanca E, Knights D, et al. Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst. Nat Commun. 2015;6:7838 pubmed publisher
  17. Shah K, Clark J, Goods B, Politano T, Mozdzierz N, Zimnisky R, et al. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng. 2015;112:2624-9 pubmed publisher
  18. Marathe S, Liu S, Brai E, Kaczarowski M, Alberi L. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry. Cell Death Differ. 2015;22:1775-84 pubmed publisher
  19. Guo L, Chen H, Li Y, Zhou Q, Sui Y. An aquaporin 3-notch1 axis in keratinocyte differentiation and inflammation. PLoS ONE. 2013;8:e80179 pubmed publisher