This is a Validated Antibody Database (VAD) review about human RAPGEF4, based on 5 published articles (read how Labome selects the articles), using RAPGEF4 antibody in all methods. It is aimed to help Labome visitors find the most suited RAPGEF4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RAPGEF4 synonym: CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496; rap guanine nucleotide exchange factor 4; RAP guanine-nucleotide-exchange factor (GEF) 4; Rap guanine nucleotide exchange factor (GEF) 4; cAMP-regulated guanine nucleotide exchange factor II; exchange factor directly activated by cAMP 2; exchange protein directly activated by cAMP 2

Knockout validation
Cell Signaling Technology
mouse monoclonal (5B1)
  • western blot knockout validation; mouse; fig 2
Cell Signaling Technology RAPGEF4 antibody (Cell signaling, 4156) was used in western blot knockout validation on mouse samples (fig 2). Int J Obes (Lond) (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-7)
  • western blot; rat; loading ...; fig 3f
In order to demonstrate that signalosomes have their own machinery to synthesize and regulate a sequestered cAMP pool, Santa Cruz Biotechnology RAPGEF4 antibody (Santa Cruz, sc-28326) was used in western blot on rat samples (fig 3f). FEBS J (2017) ncbi
Abcam
rabbit monoclonal (EPR15246)
  • western blot; human; fig 1b
Abcam RAPGEF4 antibody (Abcam, ab193665) was used in western blot on human samples (fig 1b). Mol Vis (2017) ncbi
Cell Signaling Technology
mouse monoclonal (5B1)
  • western blot; human; loading ...; fig 4c
In order to study the expression of transmembrane AC isoforms in pancreatic cancer cells, Cell Signaling Technology RAPGEF4 antibody (Cell Signaling, 4156) was used in western blot on human samples (fig 4c). Mol Carcinog (2017) ncbi
mouse monoclonal (5B1)
  • western blot knockout validation; mouse; fig 2
Cell Signaling Technology RAPGEF4 antibody (Cell signaling, 4156) was used in western blot knockout validation on mouse samples (fig 2). Int J Obes (Lond) (2017) ncbi
mouse monoclonal (5B1)
  • western blot; human; 1:2000
Cell Signaling Technology RAPGEF4 antibody (Cell Signaling Technology, 5B1) was used in western blot on human samples at 1:2000. Endocrinology (2014) ncbi
Articles Reviewed
  1. Liu L, Jiang Y, Chahine A, Curtiss E, Steinle J. Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice. Mol Vis. 2017;23:1-7 pubmed
  2. Quinn S, Graves S, Dains McGahee C, Friedman E, Hassan H, Witkowski P, et al. Adenylyl cyclase 3/adenylyl cyclase-associated protein 1 (CAP1) complex mediates the anti-migratory effect of forskolin in pancreatic cancer cells. Mol Carcinog. 2017;56:1344-1360 pubmed publisher
  3. Hwang M, Go Y, Park J, Shin S, Song S, Oh B, et al. Epac2a-null mice exhibit obesity-prone nature more susceptible to leptin resistance. Int J Obes (Lond). 2017;41:279-288 pubmed publisher
  4. Guinzberg R, Díaz Cruz A, Acosta Trujillo C, Vilchis Landeros M, Vázquez Meza H, Lozano Flores C, et al. Newly synthesized cAMP is integrated at a membrane protein complex signalosome to ensure receptor response specificity. FEBS J. 2017;284:258-276 pubmed publisher
  5. Kusama K, Yoshie M, Tamura K, Nakayama T, Nishi H, Isaka K, et al. The role of exchange protein directly activated by cyclic AMP 2-mediated calreticulin expression in the decidualization of human endometrial stromal cells. Endocrinology. 2014;155:240-8 pubmed publisher