This is a Validated Antibody Database (VAD) review about human RANKL, based on 22 published articles (read how Labome selects the articles), using RANKL antibody in all methods. It is aimed to help Labome visitors find the most suited RANKL antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RANKL synonym: CD254; ODF; OPGL; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2; sOdf

Knockout validation
R&D Systems
rat monoclonal (88227)
  • immunohistochemistry knockout validation; mouse; fig 1
  • immunohistochemistry - frozen section; mouse; fig 1
In order to investigate how TRANCE on stromal cells contributes to the differentiation and maintenance of organized lymphoid aggregates in the small intestine, R&D Systems RANKL antibody (R&D, 88227) was used in immunohistochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry - frozen section on mouse samples (fig 1). J Immunol (2007) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:200; loading ...; fig 2c
Abcam RANKL antibody (Abcam, Ab-9957) was used in western blot on mouse samples at 1:200 (fig 2c). Nat Commun (2021) ncbi
mouse monoclonal (12A668)
  • immunohistochemistry; human; 1:100; loading ...; fig 2g
Abcam RANKL antibody (AbCam, 12A668) was used in immunohistochemistry on human samples at 1:100 (fig 2g). Int J Mol Sci (2019) ncbi
mouse monoclonal (12A668)
  • western blot; mouse; loading ...; fig 5a
Abcam RANKL antibody (Abcam, ab45039) was used in western blot on mouse samples (fig 5a). BMC Musculoskelet Disord (2017) ncbi
mouse monoclonal (12A668)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1m
Abcam RANKL antibody (Abcam, 12A668) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1m). Arch Dermatol Res (2017) ncbi
mouse monoclonal (12A668)
  • western blot; mouse; fig 3
In order to infer a RANKL-dependent osteoclastogenesis after debridement of osteomyelitis coinciding with elevated B cells and simultaneously decreased osteogenesis, Abcam RANKL antibody (Abcam, ab45039) was used in western blot on mouse samples (fig 3). J Orthop Res (2017) ncbi
mouse monoclonal (12A668)
  • western blot; mouse; 1:1000; fig 4p
Abcam RANKL antibody (Abcam, ab45039) was used in western blot on mouse samples at 1:1000 (fig 4p). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 6i
In order to study the contribution of IL-10 producing B cells on periodontal inflammation and bone loss in ligature-induced experimental periodontitis, Abcam RANKL antibody (Abcam, ab62516) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 6i). Infect Immun (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:3000; fig 4
Abcam RANKL antibody (Abcam, ab9957) was used in immunohistochemistry - paraffin section on mouse samples at 1:3000 (fig 4). Mol Med Rep (2016) ncbi
mouse monoclonal (12A668)
  • immunohistochemistry - paraffin section; human; 1:400; loading ...; fig 1c
Abcam RANKL antibody (Abcam, AB45039) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 1c). Oral Surg Oral Med Oral Pathol Oral Radiol (2015) ncbi
BioLegend
mouse monoclonal (MIH24)
  • flow cytometry; human; loading ...; fig 5c
BioLegend RANKL antibody (Biolegend, MIH24) was used in flow cytometry on human samples (fig 5c). J Biol Chem (2018) ncbi
mouse monoclonal (MIH24)
  • flow cytometry; human; loading ...; fig 1e, 1f
In order to study the involvement of RANKL in decidual M2 macrophage polarization, BioLegend RANKL antibody (Biolegend, 347504) was used in flow cytometry on human samples (fig 1e, 1f). Cell Death Dis (2017) ncbi
mouse monoclonal (MIH24)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BioLegend RANKL antibody (bioLegen d, 347504) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (MIH24)
  • flow cytometry; human; loading ...; fig s2a
In order to study age-related changes in human immunity during a primary virus infection experimentally induced by immunization with live-attenuated yellow fever vaccine, BioLegend RANKL antibody (Biolegend, MIH24) was used in flow cytometry on human samples (fig s2a). J Immunol (2015) ncbi
mouse monoclonal (MIH24)
In order to describe the features of plasma cells in patients with granulomatosis with polyangiitis, BioLegend RANKL antibody (Biolegend, MIH24) was used . Arthritis Res Ther (2014) ncbi
Novus Biologicals
mouse monoclonal (8A7B9)
  • western blot; mouse; 1:200; loading ...; fig 2c
Novus Biologicals RANKL antibody (Novus, NBP2-61813) was used in western blot on mouse samples at 1:200 (fig 2c). Nat Commun (2021) ncbi
R&D Systems
domestic goat polyclonal
  • western blot; human; fig 3e
R&D Systems RANKL antibody (R&D Systems, AB626) was used in western blot on human samples (fig 3e). J Immunol (2017) ncbi
domestic goat polyclonal
  • flow cytometry; human; loading ...
In order to study the involvement of RANKL in decidual M2 macrophage polarization, R&D Systems RANKL antibody (R&D Systems, AB626) was used in flow cytometry on human samples . Cell Death Dis (2017) ncbi
domestic goat polyclonal
  • ELISA; human; 0.5 ug/ml; fig 6a
In order to discuss the use of glycosaminoglycans to treat periodontitis, R&D Systems RANKL antibody (R&D systems, AF626) was used in ELISA on human samples at 0.5 ug/ml (fig 6a). PLoS ONE (2016) ncbi
rat monoclonal (88227)
  • immunohistochemistry knockout validation; mouse; fig 1
  • immunohistochemistry - frozen section; mouse; fig 1
In order to investigate how TRANCE on stromal cells contributes to the differentiation and maintenance of organized lymphoid aggregates in the small intestine, R&D Systems RANKL antibody (R&D, 88227) was used in immunohistochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry - frozen section on mouse samples (fig 1). J Immunol (2007) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-1)
  • western blot; mouse; 1:1000; loading ...; fig 3a
Santa Cruz Biotechnology RANKL antibody (Santa Cruz Biotechnology, sc377079) was used in western blot on mouse samples at 1:1000 (fig 3a). elife (2020) ncbi
mouse monoclonal (G-1)
  • western blot; human; loading ...; fig 7a
Santa Cruz Biotechnology RANKL antibody (Santa Cruz, sc-377079) was used in western blot on human samples (fig 7a). Oncotarget (2016) ncbi
Enzo Life Sciences
mouse monoclonal (12A380)
  • immunohistochemistry; rat; 1:800; fig 5a,5c
Enzo Life Sciences RANKL antibody (Alexis Biochemicals, ALX-804-244-C100) was used in immunohistochemistry on rat samples at 1:800 (fig 5a,5c). Mol Med Rep (2015) ncbi
mouse monoclonal (12A668)
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 2b
Enzo Life Sciences RANKL antibody (Enzo Life Sciences, 12A668) was used in immunohistochemistry - paraffin section on rat samples at 1:200 (fig 2b). J Biomed Mater Res B Appl Biomater (2015) ncbi
Active Motif
mouse monoclonal (12A380)
  • western blot; mouse
Active Motif RANKL antibody (Active Motif, 40940) was used in western blot on mouse samples . J Bone Miner Res (2011) ncbi
Articles Reviewed
  1. Blomberg Jensen M, Andreassen C, Jørgensen A, Nielsen J, Juel Mortensen L, Boisen I, et al. RANKL regulates male reproductive function. Nat Commun. 2021;12:2450 pubmed publisher
  2. An J, Kerns K, Ouellette A, Robinson L, Morris H, Kaczorowski C, et al. Rapamycin rejuvenates oral health in aging mice. elife. 2020;9: pubmed publisher
  3. Scimeca M, Bonfiglio R, Menichini E, Albonici L, Urbano N, De Caro M, et al. Microcalcifications Drive Breast Cancer Occurrence and Development by Macrophage-Mediated Epithelial to Mesenchymal Transition. Int J Mol Sci. 2019;20: pubmed publisher
  4. Cribbs A, Hookway E, Wells G, Lindow M, Obad S, Oerum H, et al. Inhibition of histone H3K27 demethylases selectively modulates inflammatory phenotypes of natural killer cells. J Biol Chem. 2018;293:2422-2437 pubmed publisher
  5. Chen X, Chen J, Xu D, Zhao S, Song H, Peng Y. Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs. BMC Musculoskelet Disord. 2017;18:423 pubmed publisher
  6. Emori T, Hirose J, Ise K, Yomoda J, Kasahara M, Shinkuma T, et al. Constitutive Activation of Integrin ?9 Augments Self-Directed Hyperplastic and Proinflammatory Properties of Fibroblast-like Synoviocytes of Rheumatoid Arthritis. J Immunol. 2017;199:3427-3436 pubmed publisher
  7. Meng Y, Zhou W, Jin L, Liu L, Chang K, Mei J, et al. RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization. Cell Death Dis. 2017;8:e3105 pubmed publisher
  8. Matsuyama K, Mizutani Y, Takahashi T, Shu E, Kanoh H, Miyazaki T, et al. Enhanced dendritic cells and regulatory T cells in the dermis of porokeratosis. Arch Dermatol Res. 2017;309:749-756 pubmed publisher
  9. Wagner J, Jaurich H, Wallner C, Abraham S, Becerikli M, Dadras M, et al. Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity. J Orthop Res. 2017;35:2425-2434 pubmed publisher
  10. Xu Z, Greenblatt M, Yan G, Feng H, Sun J, Lotinun S, et al. SMURF2 regulates bone homeostasis by disrupting SMAD3 interaction with vitamin D receptor in osteoblasts. Nat Commun. 2017;8:14570 pubmed publisher
  11. Yu P, Hu Y, Liu Z, Kawai T, Taubman M, Li W, et al. Local Induction of B Cell Interleukin-10 Competency Alleviates Inflammation and Bone Loss in Ligature-Induced Experimental Periodontitis in Mice. Infect Immun. 2017;85: pubmed publisher
  12. Savage J, Pulsipher A, Rao N, Kennedy T, Prestwich G, Ryan M, et al. A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis. PLoS ONE. 2016;11:e0157310 pubmed publisher
  13. He M, Wang J, Wang G, Tian Y, Jiang L, Ren Z, et al. Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis. Mol Med Rep. 2016;14:1054-60 pubmed publisher
  14. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  15. Sánchez C, Andahur E, Valenzuela R, Castellón E, Fullá J, Ramos C, et al. Exosomes from bulk and stem cells from human prostate cancer have a differential microRNA content that contributes cooperatively over local and pre-metastatic niche. Oncotarget. 2016;7:3993-4008 pubmed publisher
  16. Schulz A, Mälzer J, Domingo C, Jürchott K, Grützkau A, Babel N, et al. Low Thymic Activity and Dendritic Cell Numbers Are Associated with the Immune Response to Primary Viral Infection in Elderly Humans. J Immunol. 2015;195:4699-711 pubmed publisher
  17. Liu Z, Liu L, Kang C, Xie Q, Zhang B, Li Y. Effects of estrogen deficiency on microstructural changes in rat alveolar bone proper and periodontal ligament. Mol Med Rep. 2015;12:3508-3514 pubmed publisher
  18. Siar C, Tsujigiwa H, Ishak I, Hussin N, Nagatsuka H, Ng K. RANK, RANKL, and OPG in recurrent solid/multicystic ameloblastoma: their distribution patterns and biologic significance. Oral Surg Oral Med Oral Pathol Oral Radiol. 2015;119:83-91 pubmed publisher
  19. Bhattarai G, Lee Y, Yi H. Peroxisome proliferator activated receptor gamma loaded dental implant improves osteogenesis of rat mandible. J Biomed Mater Res B Appl Biomater. 2015;103:587-95 pubmed publisher
  20. Mueller A, Brieske C, Schinke S, Csernok E, Gross W, Hasselbacher K, et al. Plasma cells within granulomatous inflammation display signs pointing to autoreactivity and destruction in granulomatosis with polyangiitis. Arthritis Res Ther. 2014;16:R55 pubmed publisher
  21. Rousseau J, Escriou V, Lamoureux F, Brion R, Chesneau J, Battaglia S, et al. Formulated siRNAs targeting Rankl prevent osteolysis and enhance chemotherapeutic response in osteosarcoma models. J Bone Miner Res. 2011;26:2452-62 pubmed publisher
  22. Taylor R, Patel S, Lin E, Butler B, Lake J, Newberry R, et al. Lymphotoxin-independent expression of TNF-related activation-induced cytokine by stromal cells in cryptopatches, isolated lymphoid follicles, and Peyer's patches. J Immunol. 2007;178:5659-67 pubmed