This is a Validated Antibody Database (VAD) review about human RANGAP1, based on 15 published articles (read how Labome selects the articles), using RANGAP1 antibody in all methods. It is aimed to help Labome visitors find the most suited RANGAP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RANGAP1 synonym: Fug1; RANGAP; SD

Santa Cruz Biotechnology
mouse monoclonal (C-5)
  • western blot; human; 1:50; loading ...; fig 4c
Santa Cruz Biotechnology RANGAP1 antibody (Santa Cruz, sc-28322) was used in western blot on human samples at 1:50 (fig 4c). elife (2020) ncbi
mouse monoclonal (C-5)
  • immunocytochemistry; human; loading ...; fig 6e
  • western blot; human; loading ...; fig 5b
Santa Cruz Biotechnology RANGAP1 antibody (Santa Cruz, sc-28322) was used in immunocytochemistry on human samples (fig 6e) and in western blot on human samples (fig 5b). Sci Rep (2017) ncbi
mouse monoclonal (C-5)
  • immunocytochemistry; human; 1:250; fig 3
  • western blot; human; 1:1000; fig st2
In order to study inhibition of growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex by ON 01910.Na, Santa Cruz Biotechnology RANGAP1 antibody (Santa Cruz, sc-28322) was used in immunocytochemistry on human samples at 1:250 (fig 3) and in western blot on human samples at 1:1000 (fig st2). Transl Res (2016) ncbi
mouse monoclonal (C-5)
  • western blot; human
Santa Cruz Biotechnology RANGAP1 antibody (Santa Cruz, C-5) was used in western blot on human samples . J Virol (2015) ncbi
mouse monoclonal (C-5)
  • immunohistochemistry - paraffin section; human; 1:100
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:1000
In order to study the therapeutic potential of RanGAP1 in diffuse large B-cell lymphoma, Santa Cruz Biotechnology RANGAP1 antibody (Santa Cruz, sc-28322) was used in immunohistochemistry - paraffin section on human samples at 1:100, in immunocytochemistry on human samples at 1:100 and in western blot on human samples at 1:1000. PLoS ONE (2013) ncbi
Invitrogen
mouse monoclonal (19C7)
  • western blot; human; fig 6
In order to summarize HSV-1 infection and the SUMO2 proteome, Invitrogen RANGAP1 antibody (Invitrogen, 33-0800) was used in western blot on human samples (fig 6). PLoS Pathog (2015) ncbi
mouse monoclonal (19C7)
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 5
In order to identify a new role for the pore-associated SENPs in nucleoporin homeostasis and proper configuration of the nuclear pore complex, Invitrogen RANGAP1 antibody (Zymed, 33-0800) was used in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 5). Mol Biol Cell (2014) ncbi
mouse monoclonal (19C7)
  • immunocytochemistry; human
  • western blot; human
In order to study the role of nucleoporin RanBP2/Nup358 in chromosomal alignment and mitotic integrity, Invitrogen RANGAP1 antibody (Life Technologies Corporation, 33-0800) was used in immunocytochemistry on human samples and in western blot on human samples . Cell Death Dis (2013) ncbi
mouse monoclonal (19C7)
  • immunocytochemistry; human; 1:1000; fig 8
In order to investigate the contribution of pore membrane protein-121 to nuclear pore assembly, Invitrogen RANGAP1 antibody (Zymed, 33-0800) was used in immunocytochemistry on human samples at 1:1000 (fig 8). Mol Biol Cell (2011) ncbi
mouse monoclonal (19C7)
  • western blot; human; fig 3
In order to describe a method to detect SUMOylation, Invitrogen RANGAP1 antibody (Zymed, 19C7) was used in western blot on human samples (fig 3). Nat Protoc (2009) ncbi
mouse monoclonal (19C7)
  • western blot; human
In order to investigate the cross-talk between sumoylation and ubiquitination, Invitrogen RANGAP1 antibody (Zymed Laboratories, 19C7) was used in western blot on human samples . Mol Cell Proteomics (2008) ncbi
mouse monoclonal (19C7)
  • western blot; human; fig 1
In order to investigate the target protein preferences of SUMO-1 and SUMO-2, Invitrogen RANGAP1 antibody (Zymed, 19C7) was used in western blot on human samples (fig 1). Mol Cell Proteomics (2006) ncbi
mouse monoclonal (19C7)
  • western blot; human
In order to develop a new method for proteomic studies of SUMO-modified proteins, Invitrogen RANGAP1 antibody (Zymed, 19c7) was used in western blot on human samples . Mol Cell Proteomics (2005) ncbi
mouse monoclonal (19C7)
  • immunocytochemistry; African green monkey; fig 5
In order to report quantitative and qualitative differences between SUMO-1 and SUMO-2/3 in vertebrate cells, Invitrogen RANGAP1 antibody (Zymed Laboratories, clone 19C7) was used in immunocytochemistry on African green monkey samples (fig 5). J Biol Chem (2000) ncbi
Abcam
domestic rabbit monoclonal (EPR3295)
  • western blot; human; 1:200; loading ...; fig 1g
Abcam RANGAP1 antibody (Abcam, ab92360) was used in western blot on human samples at 1:200 (fig 1g). Nat Commun (2017) ncbi
Articles Reviewed
  1. Hayes L, Duan L, Bowen K, Kalab P, Rothstein J. C9orf72 arginine-rich dipeptide repeat proteins disrupt karyopherin-mediated nuclear import. elife. 2020;9: pubmed publisher
  2. Oldrini B, Hsieh W, Erdjument Bromage H, Codega P, Carro M, Curiel García A, et al. EGFR feedback-inhibition by Ran-binding protein 6 is disrupted in cancer. Nat Commun. 2017;8:2035 pubmed publisher
  3. Dattilo V, D Antona L, Talarico C, Capula M, Catalogna G, Iuliano R, et al. SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation. Sci Rep. 2017;7:45361 pubmed publisher
  4. Dai Y, Hung L, Chen R, Lai C, Chang K. ON 01910.Na inhibits growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex. Transl Res. 2016;175:129-143.e13 pubmed publisher
  5. Sloan E, Tatham M, Groslambert M, Glass M, Orr A, Hay R, et al. Analysis of the SUMO2 Proteome during HSV-1 Infection. PLoS Pathog. 2015;11:e1005059 pubmed publisher
  6. Sohn S, Bridges R, Hearing P. Proteomic analysis of ubiquitin-like posttranslational modifications induced by the adenovirus E4-ORF3 protein. J Virol. 2015;89:1744-55 pubmed publisher
  7. Chang K, Chang W, Chang Y, Hung L, Lai C, Yeh Y, et al. Ran GTPase-activating protein 1 is a therapeutic target in diffuse large B-cell lymphoma. PLoS ONE. 2013;8:e79863 pubmed publisher
  8. Chow K, Elgort S, Dasso M, Powers M, Ullman K. The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Mol Biol Cell. 2014;25:160-8 pubmed publisher
  9. Hashizume C, Kobayashi A, Wong R. Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe. Cell Death Dis. 2013;4:e854 pubmed publisher
  10. Funakoshi T, Clever M, Watanabe A, Imamoto N. Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase nuclear pore complex assembly. Mol Biol Cell. 2011;22:1058-69 pubmed publisher
  11. Tatham M, Rodriguez M, Xirodimas D, Hay R. Detection of protein SUMOylation in vivo. Nat Protoc. 2009;4:1363-71 pubmed publisher
  12. Schimmel J, Larsen K, Matic I, van Hagen M, Cox J, Mann M, et al. The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle. Mol Cell Proteomics. 2008;7:2107-22 pubmed publisher
  13. Vertegaal A, Andersen J, Ogg S, Hay R, Mann M, Lamond A. Distinct and overlapping sets of SUMO-1 and SUMO-2 target proteins revealed by quantitative proteomics. Mol Cell Proteomics. 2006;5:2298-310 pubmed
  14. Rosas Acosta G, Russell W, Deyrieux A, Russell D, Wilson V. A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers. Mol Cell Proteomics. 2005;4:56-72 pubmed
  15. Saitoh H, Hinchey J. Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3. J Biol Chem. 2000;275:6252-8 pubmed