This is a Validated Antibody Database (VAD) review about human RAD21, based on 10 published articles (read how Labome selects the articles), using RAD21 antibody in all methods. It is aimed to help Labome visitors find the most suited RAD21 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RAD21 synonym: CDLS4; HR21; HRAD21; MCD1; MGS; NXP1; SCC1; hHR21

Abcam
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; loading ...; fig 3c
Abcam RAD21 antibody (Abcam, ab992) was used in immunocytochemistry on mouse samples at 1:100 (fig 3c). Life Sci Alliance (2020) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig e1h
Abcam RAD21 antibody (Abcam, ab992) was used in chromatin immunoprecipitation on mouse samples (fig e1h). Nature (2019) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1d
Abcam RAD21 antibody (Abcam, ab992) was used in ChIP-Seq on mouse samples (fig 1d). Cell (2019) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig e6j
Abcam RAD21 antibody (Abcam, ab992) was used in ChIP-Seq on mouse samples (fig e6j). Nature (2019) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1c
Abcam RAD21 antibody (Abcam, ab992) was used in ChIP-Seq on mouse samples (fig 1c). Nature (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; loading ...; fig 6
In order to characterize REC8, RAD21L, and SMC1beta knockout mice, Abcam RAD21 antibody (Abcam, ab154769) was used in immunocytochemistry on mouse samples at 1:100 (fig 6). PLoS Genet (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-12)
  • western blot; human; loading ...; fig s3b
In order to report that inhibition of PP2A kills PLK1-overexpressing breast, pancreatic, ovarian, glioblastoma, and prostate cancer cells, Santa Cruz Biotechnology RAD21 antibody (Santa Cruz, sc-166973) was used in western blot on human samples (fig s3b). Oncotarget (2016) ncbi
mouse monoclonal (B-2)
  • western blot; human; fig 4
Santa Cruz Biotechnology RAD21 antibody (Santa Cruz Biotechnology, B-2) was used in western blot on human samples (fig 4). PLoS Genet (2016) ncbi
Active Motif
mouse monoclonal (52A311)
  • western blot; mouse; loading ...; fig 2d
In order to explore the contributions of Brd2 and Brd4 during mouse T helper 17 cell differentiation, Active Motif RAD21 antibody (Active Motif, 39384) was used in western blot on mouse samples (fig 2d). Mol Cell (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • ChIP-Seq; human; 1-2 ug / 500 ul chr; loading ...; fig 4c
Cell Signaling Technology RAD21 antibody (Cell Signaling, 4321) was used in ChIP-Seq on human samples at 1-2 ug / 500 ul chr (fig 4c). Cell Rep (2019) ncbi
Articles Reviewed
  1. McNicoll F, Kühnel A, Biswas U, Hempel K, Whelan G, Eichele G, et al. Meiotic sex chromosome cohesion and autosomal synapsis are supported by Esco2. Life Sci Alliance. 2020;3: pubmed publisher
  2. Senigl F, Maman Y, Dinesh R, Alinikula J, Seth R, Pecnova L, et al. Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation. Cell Rep. 2019;29:3902-3915.e8 pubmed publisher
  3. Zhang H, Emerson D, Gilgenast T, Titus K, Lan Y, Huang P, et al. Chromatin structure dynamics during the mitosis-to-G1 phase transition. Nature. 2019;576:158-162 pubmed publisher
  4. Kaaij L, Mohn F, van der Weide R, de Wit E, B hler M. The ChAHP Complex Counteracts Chromatin Looping at CTCF Sites that Emerged from SINE Expansions in Mouse. Cell. 2019;178:1437-1451.e14 pubmed publisher
  5. MONAHAN K, HORTA A, Lomvardas S. LHX2- and LDB1-mediated trans interactions regulate olfactory receptor choice. Nature. 2019;565:448-453 pubmed publisher
  6. Busslinger G, Stocsits R, van der Lelij P, Axelsson E, Tedeschi A, Galjart N, et al. Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl. Nature. 2017;544:503-507 pubmed publisher
  7. Cheung K, Zhang F, Jaganathan A, Sharma R, Zhang Q, Konuma T, et al. Distinct Roles of Brd2 and Brd4 in Potentiating the Transcriptional Program for Th17 Cell Differentiation. Mol Cell. 2017;65:1068-1080.e5 pubmed publisher
  8. Biswas U, Hempel K, Llano E, Pendas A, Jessberger R. Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis. PLoS Genet. 2016;12:e1006389 pubmed publisher
  9. Cunningham C, Li S, Vizeacoumar F, Bhanumathy K, Lee J, Parameswaran S, et al. Therapeutic relevance of the protein phosphatase 2A in cancer. Oncotarget. 2016;7:61544-61561 pubmed publisher
  10. Kim J, He X, Orr B, Wutz G, Hill V, Peters J, et al. Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. PLoS Genet. 2016;12:e1005865 pubmed publisher