This is a Validated Antibody Database (VAD) review about human RACK1, based on 18 published articles (read how Labome selects the articles), using RACK1 antibody in all methods. It is aimed to help Labome visitors find the most suited RACK1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RACK1 synonym: GNB2L1; Gnb2-rs1; H12.3; HLC-7; PIG21; receptor of activated protein C kinase 1; cell proliferation-inducing gene 21 protein; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding protein beta polypeptide 2-like 1; guanine nucleotide-binding protein subunit beta-2-like 1; guanine nucleotide-binding protein subunit beta-like protein 12.3; human lung cancer oncogene 7 protein; lung cancer oncogene 7; proliferation-inducing gene 21; protein homologous to chicken B complex protein, guanine nucleotide binding; receptor of activated protein kinase C 1; small ribosomal subunit protein RACK1

Knockout validation
Cell Signaling Technology
rabbit monoclonal (D59D5)
  • western blot knockout validation; human; fig 1b
Cell Signaling Technology RACK1 antibody (CST, 5432) was used in western blot knockout validation on human samples (fig 1b). Nature (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-3)
  • western blot; human; loading ...; fig 4c
Santa Cruz Biotechnology RACK1 antibody (Santa Cruz, sc-17754) was used in western blot on human samples (fig 4c). Autophagy (2016) ncbi
mouse monoclonal (B-3)
  • western blot; human; loading ...; fig 2b
Santa Cruz Biotechnology RACK1 antibody (Santa Cruz, SC-17754) was used in western blot on human samples (fig 2b). Oncotarget (2016) ncbi
mouse monoclonal (B-3)
  • western blot; human; loading ...; fig 1b
Santa Cruz Biotechnology RACK1 antibody (Santa Cruz Biotechnology, sc-17754) was used in western blot on human samples (fig 1b). Nucleic Acids Res (2016) ncbi
mouse monoclonal (B-3)
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 1
  • western blot; mouse; 1:1000; fig 1
Santa Cruz Biotechnology RACK1 antibody (Santa Cruz Biotechnology, sc-17754) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1) and in western blot on mouse samples at 1:1000 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (B-3)
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Santa Cruz Biotechnology RACK1 antibody (Santa Cruz, sc-17754) was used in immunoprecipitation on human samples , in immunocytochemistry on human samples and in western blot on human samples . J Biol Chem (2013) ncbi
Abcam
rabbit monoclonal (EPR7388)
  • western blot; mouse; fig 2a
Abcam RACK1 antibody (Abcam, ab129084) was used in western blot on mouse samples (fig 2a). Transl Psychiatry (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; Asian tiger mosquito; 1:10,000
Abcam RACK1 antibody (AbCam, ab62735) was used in immunocytochemistry on Asian tiger mosquito samples at 1:10,000. J Proteomics (2015) ncbi
rabbit polyclonal
  • western blot; human
Abcam RACK1 antibody (Abcam, ab62735) was used in western blot on human samples . Int J Nanomedicine (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100
  • western blot; mouse
In order to study the physical interaction of annexin A7 and RACK1 and the significance for hepatocarcinoma metastasis, Abcam RACK1 antibody (Abcam, ab-62735) was used in immunocytochemistry on mouse samples at 1:100 and in western blot on mouse samples . Biochem Biophys Res Commun (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D59D5)
  • western blot knockout validation; human; fig 1b
Cell Signaling Technology RACK1 antibody (CST, 5432) was used in western blot knockout validation on human samples (fig 1b). Nature (2017) ncbi
rabbit monoclonal (D59D5)
  • immunoprecipitation; human; loading ...; fig 5e
  • western blot; human; loading ...; fig 5e
Cell Signaling Technology RACK1 antibody (Cell Signaling, 5432) was used in immunoprecipitation on human samples (fig 5e) and in western blot on human samples (fig 5e). Sci Signal (2017) ncbi
rabbit monoclonal (D59D5)
  • western blot; human; 1:1000; loading ...; fig sf10a
In order to describe a function for NQO1 in stabilizing HIF-1alpha, Cell Signaling Technology RACK1 antibody (Cell Signaling, 5432S) was used in western blot on human samples at 1:1000 (fig sf10a). Nat Commun (2016) ncbi
rabbit monoclonal (D59D5)
  • western blot; mouse; 1:2000; fig 2
  • western blot; human; 1:2000; fig 2
In order to study the composition of the protein aggregate hallmark of Alexander disease called rosenthal fibers, Cell Signaling Technology RACK1 antibody (Cell Signaling, D59D5) was used in western blot on mouse samples at 1:2000 (fig 2) and in western blot on human samples at 1:2000 (fig 2). J Proteome Res (2016) ncbi
BD Biosciences
mouse monoclonal (20/RACK1)
  • western blot; mouse; loading ...; fig 2i
  • immunohistochemistry - paraffin section; human; loading ...; fig 3a
  • immunocytochemistry; human; loading ...; fig 5b
  • western blot; human; loading ...; fig 1a, 2h
BD Biosciences RACK1 antibody (BD, 610178) was used in western blot on mouse samples (fig 2i), in immunohistochemistry - paraffin section on human samples (fig 3a), in immunocytochemistry on human samples (fig 5b) and in western blot on human samples (fig 1a, 2h). Theranostics (2019) ncbi
mouse monoclonal (20/RACK1)
  • immunoprecipitation; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 4a
BD Biosciences RACK1 antibody (BD Biosciences, 61078) was used in immunoprecipitation on human samples (fig 4a) and in western blot on human samples (fig 4a). Mol Cell Biol (2017) ncbi
mouse monoclonal (20/RACK1)
  • immunocytochemistry; human; fig s3
BD Biosciences RACK1 antibody (BD, 610178) was used in immunocytochemistry on human samples (fig s3). Nat Commun (2015) ncbi
mouse monoclonal (20/RACK1)
  • western blot; human; 1:1000
BD Biosciences RACK1 antibody (BD Biosciences, 610177) was used in western blot on human samples at 1:1000. Br J Pharmacol (2015) ncbi
mouse monoclonal (20/RACK1)
  • immunocytochemistry; human; 1:200
BD Biosciences RACK1 antibody (BD, 610177) was used in immunocytochemistry on human samples at 1:200. Nucleic Acids Res (2014) ncbi
Articles Reviewed
  1. Cao J, Zhao M, Liu J, Zhang X, Pei Y, Wang J, et al. RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog. Theranostics. 2019;9:811-828 pubmed publisher
  2. Jha S, Rollins M, Fuchs G, Procter D, HALL E, Cozzolino K, et al. Trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase. Nature. 2017;546:651-655 pubmed publisher
  3. CAROMILE L, Dortche K, Rahman M, Grant C, Stoddard C, Ferrer F, et al. PSMA redirects cell survival signaling from the MAPK to the PI3K-AKT pathways to promote the progression of prostate cancer. Sci Signal. 2017;10: pubmed publisher
  4. Shizu R, Osabe M, Perera L, Moore R, Sueyoshi T, Negishi M. Phosphorylated Nuclear Receptor CAR Forms a Homodimer To Repress Its Constitutive Activity for Ligand Activation. Mol Cell Biol. 2017;37: pubmed publisher
  5. Oh E, Kim J, Kim J, Kim S, Lee J, Hong S, et al. NQO1 inhibits proteasome-mediated degradation of HIF-1α. Nat Commun. 2016;7:13593 pubmed publisher
  6. Wang J, Zhang J, Lee Y, Koh P, Ng S, Bao F, et al. Quantitative chemical proteomics profiling of de novo protein synthesis during starvation-mediated autophagy. Autophagy. 2016;12:1931-1944 pubmed
  7. Kim Y, Lee M, Kim H, Kim J. Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated. Oncotarget. 2016;7:80350-80362 pubmed publisher
  8. Heaven M, Flint D, Randall S, Sosunov A, Wilson L, Barnes S, et al. Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease. J Proteome Res. 2016;15:2265-82 pubmed publisher
  9. Lyons S, Achorn C, Kedersha N, Anderson P, Ivanov P. YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression. Nucleic Acids Res. 2016;44:6949-60 pubmed publisher
  10. Liu L, Zhu J, Zhou L, Wan L. RACK1 promotes maintenance of morphine-associated memory via activation of an ERK-CREB dependent pathway in hippocampus. Sci Rep. 2016;6:20183 pubmed publisher
  11. Corcoran K, Leaderbrand K, Jovasevic V, Guedea A, Kassam F, Radulovic J. Regulation of fear extinction versus other affective behaviors by discrete cortical scaffolding complexes associated with NR2B and PKA signaling. Transl Psychiatry. 2015;5:e657 pubmed publisher
  12. Poulter N, Pollitt A, Davies A, Malinova D, Nash G, Hannon M, et al. Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex. Nat Commun. 2015;6:7254 pubmed publisher
  13. Pinto A, Malacrida B, Oieni J, Serafini M, Davin A, Galbiati V, et al. DHEA modulates the effect of cortisol on RACK1 expression via interference with the splicing of the glucocorticoid receptor. Br J Pharmacol. 2015;172:2918-27 pubmed publisher
  14. González Calixto C, Cázares Raga F, Cortes Martinez L, del Angel R, Medina Ramírez F, Mosso C, et al. AealRACK1 expression and localization in response to stress in C6/36 HT mosquito cells. J Proteomics. 2015;119:45-60 pubmed publisher
  15. Coppari E, Yamada T, Bizzarri A, Beattie C, Cannistraro S. A nanotechnological, molecular-modeling, and immunological approach to study the interaction of the anti-tumorigenic peptide p28 with the p53 family of proteins. Int J Nanomedicine. 2014;9:1799-813 pubmed publisher
  16. Kaehler C, Isensee J, Hucho T, Lehrach H, Krobitsch S. 5-Fluorouracil affects assembly of stress granules based on RNA incorporation. Nucleic Acids Res. 2014;42:6436-47 pubmed publisher
  17. Du Y, Meng J, Huang Y, Wu J, Wang B, Ibrahim M, et al. Guanine nucleotide-binding protein subunit beta-2-like 1, a new Annexin A7 interacting protein. Biochem Biophys Res Commun. 2014;445:58-63 pubmed publisher
  18. Dave J, Kang H, Abbey C, Maxwell S, Bayless K. Proteomic profiling of endothelial invasion revealed receptor for activated C kinase 1 (RACK1) complexed with vimentin to regulate focal adhesion kinase (FAK). J Biol Chem. 2013;288:30720-33 pubmed publisher