This is a Validated Antibody Database (VAD) review about human PYK2, based on 31 published articles (read how Labome selects the articles), using PYK2 antibody in all methods. It is aimed to help Labome visitors find the most suited PYK2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PYK2 synonym: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK; protein-tyrosine kinase 2-beta; CAK-beta; FADK 2; PTK2B protein tyrosine kinase 2 beta; calcium-dependent tyrosine kinase; calcium-regulated non-receptor proline-rich tyrosine kinase; cell adhesion kinase beta; focal adhesion kinase 2; proline-rich tyrosine kinase 2; protein kinase B; related adhesion focal tyrosine kinase

Invitrogen
mouse monoclonal (5E2D5)
  • western blot; human; loading ...; fig s1
In order to explore the effect of focal adhesion kinase on migration and cellular mechanics, Invitrogen PYK2 antibody (life technology, 5E2D5) was used in western blot on human samples (fig s1). Sci Rep (2017) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000; fig 3c
In order to assess the role of Pyk2 in phenotype modulation in rat carotid artery smooth muscle cells and in cultured intact arteries, Invitrogen PYK2 antibody (Invitrogen, 44-618G) was used in western blot on rat samples at 1:1000 (fig 3c). J Cell Physiol (2017) ncbi
rabbit monoclonal (17HCLC)
  • western blot; mouse; fig 2
In order to assess whether proline-rich tyrosine kinase 2 contributes to complement receptor 3-dependent phagocytosis and Fcx03B3; receptor-mediated uptake in RAW 264.7 macrophages, Invitrogen PYK2 antibody (Life Technologies, 17HCLC) was used in western blot on mouse samples (fig 2). J Innate Immun (2016) ncbi
rabbit recombinant (9H12L1)
  • flow cytometry; human; loading ...; fig s1a
In order to investigate cross-talk between Alzheimer loci, Invitrogen PYK2 antibody (Life Technologies, 9H12L1) was used in flow cytometry on human samples (fig s1a). Nat Neurosci (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 5d
In order to investigate mechanisms of tamoxifen resistance, Invitrogen PYK2 antibody (Life Technologies, 44-618A1) was used in immunocytochemistry on human samples (fig 5d). Mol Cell Proteomics (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
In order to examine how microglia promote glioma migration, Invitrogen PYK2 antibody (Invitrogen, 44636G) was used in western blot on mouse samples at 1:1000. PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
In order to examine the role of the calcium- and integrin-activated proline-rich tyrosine kinase 2 in stretch-induced responses of the rat portal vein, Invitrogen PYK2 antibody (Invitrogen, 44-618G) was used in western blot on rat samples at 1:1000. Physiol Rep (2014) ncbi
rabbit polyclonal
  • western blot; mouse
In order to assess broad patterns of signaling pathway activity during in vitro oocyte aging, Invitrogen PYK2 antibody (Invitrogen, 44632G) was used in western blot on mouse samples . Mol Reprod Dev (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
In order to investigate the effect of mechanical force on the T cell receptor, Invitrogen PYK2 antibody (Invitrogen, 44-618G) was used in immunocytochemistry on human samples . Proc Natl Acad Sci U S A (2014) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to study COLXV in pancreatic adenocarcinoma cells, Invitrogen PYK2 antibody (Life Technologies, 44636G) was used in western blot on human samples (fig 4). PLoS ONE (2013) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:200; fig 5
In order to study the role of beta1 integrin in long-term potentiation and the consolidation of memory, Invitrogen PYK2 antibody (Invitrogen, 44?C618G) was used in immunohistochemistry on rat samples at 1:200 (fig 5). J Neurosci (2012) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:250-500; fig 6
In order to identify the physical stimuli mediating demyelination and test if integrins plays a role in the demyelinating response, Invitrogen PYK2 antibody (Invitrogen, #44-618G) was used in immunohistochemistry on rat samples at 1:250-500 (fig 6). Ann Neurol (2012) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3
In order to determine the role of FAK activity during development, Invitrogen PYK2 antibody (Invitrogen, 44-618G) was used in western blot on mouse samples (fig 3). J Biol Chem (2010) ncbi
rabbit polyclonal
In order to investigate FAK signaling in the regulation of extracellular matrix-induced osteogenic differentiation of human mesenchymal stem cells, Invitrogen PYK2 antibody (Biosource, 44-618G) was used . Exp Cell Res (2007) ncbi
rabbit polyclonal
In order to report that FAK is an important mediator of laminin-5-induced osteogenic differentiation of human mesenchymal stem cells, Invitrogen PYK2 antibody (Biosource, 44-618G) was used . J Cell Biochem (2007) ncbi
Abcam
rabbit monoclonal (YE353)
  • western blot; mouse; loading ...; fig 6d
Abcam PYK2 antibody (Abcam, YE353) was used in western blot on mouse samples (fig 6d). Sci Signal (2018) ncbi
rabbit monoclonal (YE353)
  • immunocytochemistry; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3c
In order to assess the effects of thrombomodulin on monocyte differentiation, Abcam PYK2 antibody (Abcam, ab32571) was used in immunocytochemistry on human samples (fig 3d) and in western blot on human samples (fig 3c). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 3a
In order to assess the effects of thrombomodulin on monocyte differentiation, Abcam PYK2 antibody (Abcam, ab4801) was used in western blot on human samples (fig 3a). Sci Rep (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (14F6)
  • western blot; mouse; loading ...; fig 12a
In order to show that PTI-125 therapeutically alters filamin A and reverses proteopathy, Santa Cruz Biotechnology PYK2 antibody (SantaCruz, SC-81512) was used in western blot on mouse samples (fig 12a). Neurobiol Aging (2017) ncbi
mouse monoclonal (D-1)
  • western blot; mouse; loading ...; fig 7a
Santa Cruz Biotechnology PYK2 antibody (SantaCruz, sc-365201) was used in western blot on mouse samples (fig 7a). J Exp Med (2016) ncbi
MilliporeSigma
rabbit polyclonal
  • western blot; rat; 1:1000; fig 3c
In order to assess the role of Pyk2 in phenotype modulation in rat carotid artery smooth muscle cells and in cultured intact arteries, MilliporeSigma PYK2 antibody (Sigma, P3902) was used in western blot on rat samples at 1:1000 (fig 3c). J Cell Physiol (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; fig 4
  • immunohistochemistry - free floating section; human; fig 4
In order to identify PTK2B as an in vivo modulator and early marker of Tau pathology due to functional screening of Alzheimer risk loci, MilliporeSigma PYK2 antibody (Sigma, SAB4300468) was used in immunohistochemistry - free floating section on mouse samples (fig 4) and in immunohistochemistry - free floating section on human samples (fig 4). Mol Psychiatry (2017) ncbi
rabbit polyclonal
  • western blot; rat; 1:1500
MilliporeSigma PYK2 antibody (Sigma, SAB4500837) was used in western blot on rat samples at 1:1500. J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
In order to examine the role of the calcium- and integrin-activated proline-rich tyrosine kinase 2 in stretch-induced responses of the rat portal vein, MilliporeSigma PYK2 antibody (Sigma, P3902) was used in western blot on rat samples at 1:1000. Physiol Rep (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 4a
In order to study the the activation of T lymphocyte death by Ebola virus glycoprotein, Cell Signaling Technology PYK2 antibody (Cell Signaling, 3291S) was used in western blot on human samples (fig 4a). PLoS Pathog (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 4a
In order to study the the activation of T lymphocyte death by Ebola virus glycoprotein, Cell Signaling Technology PYK2 antibody (Cell Signaling, 3090S) was used in western blot on human samples (fig 4a). PLoS Pathog (2017) ncbi
mouse monoclonal (5E2)
  • western blot; human; loading ...; fig 7b
Cell Signaling Technology PYK2 antibody (Cell Signaling, 3480) was used in western blot on human samples (fig 7b). PLoS ONE (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 7a
  • western blot; human; loading ...; fig 7b
Cell Signaling Technology PYK2 antibody (Cell Signaling, 3291) was used in immunocytochemistry on human samples (fig 7a) and in western blot on human samples (fig 7b). PLoS ONE (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 6
In order to study the coupling of cellular prion protein to intracellular signaling in Alzheimer's disease by metabotropic glutamate receptor 5, Cell Signaling Technology PYK2 antibody (Cell Signalling, 3291) was used in western blot on mouse samples at 1:1000 (fig 6). Brain (2016) ncbi
mouse monoclonal (5E2)
  • western blot; mouse; 1:1000; fig 1
  • western blot; rat; 1:1000; fig 2
In order to study the coupling of cellular prion protein to intracellular signaling in Alzheimer's disease by metabotropic glutamate receptor 5, Cell Signaling Technology PYK2 antibody (Cell Signalling, 3480) was used in western blot on mouse samples at 1:1000 (fig 1) and in western blot on rat samples at 1:1000 (fig 2). Brain (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Cell Signaling Technology PYK2 antibody (Cell Signaling, 3292) was used in western blot on human samples at 1:1000. J Biol Chem (2015) ncbi
mouse monoclonal (5E2)
  • western blot; human; fig 6c
In order to investigate mechanisms of tamoxifen resistance, Cell Signaling Technology PYK2 antibody (Cell Signaling Technology, 3480S) was used in western blot on human samples (fig 6c). Mol Cell Proteomics (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 5a
In order to investigate mechanisms of tamoxifen resistance, Cell Signaling Technology PYK2 antibody (Cell Signaling Technology, 3291) was used in western blot on human samples (fig 5a). Mol Cell Proteomics (2015) ncbi
mouse monoclonal (5E2)
  • immunocytochemistry; mouse; 1:1000
In order to examine how microglia promote glioma migration, Cell Signaling Technology PYK2 antibody (Cell Signaling, 3480S) was used in immunocytochemistry on mouse samples at 1:1000. PLoS ONE (2015) ncbi
mouse monoclonal (5E2)
  • western blot; mouse; 1:1000; fig  1
Cell Signaling Technology PYK2 antibody (Cell Signalling, 3480) was used in western blot on mouse samples at 1:1000 (fig  1). J Mol Cell Cardiol (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig  1
Cell Signaling Technology PYK2 antibody (Cell Signalling, 3291) was used in western blot on mouse samples at 1:1000 (fig  1). J Mol Cell Cardiol (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology PYK2 antibody (Cell Signaling, 3291) was used in western blot on human samples . Mol Cell Endocrinol (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology PYK2 antibody (Cell Signaling Technologies, 3291) was used in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (5E2)
  • western blot; human
Cell Signaling Technology PYK2 antibody (Cell Signaling Technologies, 3480) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
  • immunocytochemistry; mouse
  • western blot; mouse
Cell Signaling Technology PYK2 antibody (Cell Signaling, 3291) was used in immunohistochemistry - paraffin section on mouse samples , in immunocytochemistry on mouse samples and in western blot on mouse samples . Cancer Res (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig s7a
Cell Signaling Technology PYK2 antibody (Cell Signaling, 3291) was used in western blot on mouse samples (fig s7a). Nat Immunol (2012) ncbi
mouse monoclonal (5E2)
  • western blot; mouse; fig s7b
Cell Signaling Technology PYK2 antibody (Cell Signaling, 5E2) was used in western blot on mouse samples (fig s7b). Nat Immunol (2012) ncbi
EMD Millipore
rabbit polyclonal
  • chromatin immunoprecipitation; rat
EMD Millipore PYK2 antibody (Millipore, 06-559) was used in chromatin immunoprecipitation on rat samples . FASEB J (2014) ncbi
Articles Reviewed
  1. Roy N, MacKay J, Robertson T, Hammer D, Burkhardt J. Crk adaptor proteins mediate actin-dependent T cell migration and mechanosensing induced by the integrin LFA-1. Sci Signal. 2018;11: pubmed publisher
  2. Iampietro M, Younan P, Nishida A, Dutta M, Lubaki N, Santos R, et al. Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathog. 2017;13:e1006397 pubmed publisher
  3. Wang H, Lee K, Pei Z, Khan A, Bakshi K, Burns L. PTI-125 binds and reverses an altered conformation of filamin A to reduce Alzheimer's disease pathogenesis. Neurobiol Aging. 2017;55:99-114 pubmed publisher
  4. Eppler F, Quast T, Kolanus W. Dynamin2 controls Rap1 activation and integrin clustering in human T lymphocyte adhesion. PLoS ONE. 2017;12:e0172443 pubmed publisher
  5. Mierke C, Fischer T, Puder S, Kunschmann T, Soetje B, Ziegler W. Focal adhesion kinase activity is required for actomyosin contractility-based invasion of cells into dense 3D matrices. Sci Rep. 2017;7:42780 pubmed publisher
  6. Grossi M, Bhattachariya A, Nordström I, Turczynska K, Svensson D, Albinsson S, et al. Pyk2 inhibition promotes contractile differentiation in arterial smooth muscle. J Cell Physiol. 2017;232:3088-3102 pubmed publisher
  7. Tsai C, Lin Y, Huang C, Shih C, Tsai Y, Tsao N, et al. Thrombomodulin regulates monocye differentiation via PKC? and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep. 2016;6:38421 pubmed publisher
  8. Hashimoto Tane A, Sakuma M, Ike H, Yokosuka T, Kimura Y, Ohara O, et al. Micro-adhesion rings surrounding TCR microclusters are essential for T cell activation. J Exp Med. 2016;213:1609-25 pubmed publisher
  9. Dourlen P, Fernandez Gomez F, Dupont C, Grenier Boley B, Bellenguez C, Obriot H, et al. Functional screening of Alzheimer risk loci identifies PTK2B as an in vivo modulator and early marker of Tau pathology. Mol Psychiatry. 2017;22:874-883 pubmed publisher
  10. Paone C, Rodrigues N, Ittner E, Santos C, Buntru A, Hauck C. The Tyrosine Kinase Pyk2 Contributes to Complement-Mediated Phagocytosis in Murine Macrophages. J Innate Immun. 2016;8:437-51 pubmed publisher
  11. Haas L, Salazar S, Kostylev M, Um J, Kaufman A, Strittmatter S. Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain. 2016;139:526-46 pubmed publisher
  12. Chan G, White C, Winn P, Cimpean M, Replogle J, Glick L, et al. CD33 modulates TREM2: convergence of Alzheimer loci. Nat Neurosci. 2015;18:1556-8 pubmed publisher
  13. Higa Nakamine S, Maeda N, Toku S, Yamamoto H. Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone. J Biol Chem. 2015;290:25974-85 pubmed publisher
  14. Wu X, Zahari M, Renuse S, Nirujogi R, Kim M, Manda S, et al. Phosphoproteomic Analysis Identifies Focal Adhesion Kinase 2 (FAK2) as a Potential Therapeutic Target for Tamoxifen Resistance in Breast Cancer. Mol Cell Proteomics. 2015;14:2887-900 pubmed publisher
  15. Rolón Reyes K, Kucheryavykh Y, Cubano L, Inyushin M, Skatchkov S, Eaton M, et al. Microglia Activate Migration of Glioma Cells through a Pyk2 Intracellular Pathway. PLoS ONE. 2015;10:e0131059 pubmed publisher
  16. Revuelta López E, Cal R, Herraiz Martínez A, De Gonzalo Calvo D, Nasarre L, Roura S, et al. Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes. J Mol Cell Cardiol. 2015;85:25-36 pubmed publisher
  17. Chien P, Lin C, Hsiao L, Yang C. c-Src/Pyk2/EGFR/PI3K/Akt/CREB-activated pathway contributes to human cardiomyocyte hypertrophy: Role of COX-2 induction. Mol Cell Endocrinol. 2015;409:59-72 pubmed publisher
  18. Mills R, Mita M, Nakagawa J, Shoji M, Sutherland C, Walsh M. A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle. J Biol Chem. 2015;290:8677-92 pubmed publisher
  19. Bhattachariya A, Turczyńska K, Grossi M, Nordström I, Buckbinder L, Albinsson S, et al. PYK2 selectively mediates signals for growth versus differentiation in response to stretch of spontaneously active vascular smooth muscle. Physiol Rep. 2014;2: pubmed publisher
  20. McGinnis L, Pelech S, Kinsey W. Post-ovulatory aging of oocytes disrupts kinase signaling pathways and lysosome biogenesis. Mol Reprod Dev. 2014;81:928-45 pubmed publisher
  21. Velenosi T, Feere D, Sohi G, Hardy D, Urquhart B. Decreased nuclear receptor activity and epigenetic modulation associates with down-regulation of hepatic drug-metabolizing enzymes in chronic kidney disease. FASEB J. 2014;28:5388-97 pubmed publisher
  22. Yoon H, Choi Y, Song J, Do I, Kang S, Ko Y, et al. Targeted inhibition of FAK, PYK2 and BCL-XL synergistically enhances apoptosis in ovarian clear cell carcinoma cell lines. PLoS ONE. 2014;9:e88587 pubmed publisher
  23. Bashour K, Gondarenko A, Chen H, Shen K, Liu X, Huse M, et al. CD28 and CD3 have complementary roles in T-cell traction forces. Proc Natl Acad Sci U S A. 2014;111:2241-6 pubmed publisher
  24. Aguilera K, Rivera L, Hur H, Carbon J, Toombs J, Goldstein C, et al. Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma. Cancer Res. 2014;74:1032-44 pubmed publisher
  25. Clementz A, Mutolo M, Leir S, Morris K, Kucybala K, Harris H, et al. Collagen XV inhibits epithelial to mesenchymal transition in pancreatic adenocarcinoma cells. PLoS ONE. 2013;8:e72250 pubmed publisher
  26. Babayan A, Kramar E, Barrett R, Jafari M, Haettig J, Chen L, et al. Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation. J Neurosci. 2012;32:12854-61 pubmed publisher
  27. Lin M, Frieboes L, Forootan M, Palispis W, Mozaffar T, Jafari M, et al. Biophysical stimulation induces demyelination via an integrin-dependent mechanism. Ann Neurol. 2012;72:112-23 pubmed publisher
  28. Jabara H, McDonald D, Janssen E, Massaad M, Ramesh N, Borzutzky A, et al. DOCK8 functions as an adaptor that links TLR-MyD88 signaling to B cell activation. Nat Immunol. 2012;13:612-20 pubmed publisher
  29. Lim S, Chen X, Tomar A, Miller N, Yoo J, Schlaepfer D. Knock-in mutation reveals an essential role for focal adhesion kinase activity in blood vessel morphogenesis and cell motility-polarity but not cell proliferation. J Biol Chem. 2010;285:21526-36 pubmed publisher
  30. Salasznyk R, Klees R, Williams W, Boskey A, Plopper G. Focal adhesion kinase signaling pathways regulate the osteogenic differentiation of human mesenchymal stem cells. Exp Cell Res. 2007;313:22-37 pubmed
  31. Salasznyk R, Klees R, Boskey A, Plopper G. Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5. J Cell Biochem. 2007;100:499-514 pubmed