This is a Validated Antibody Database (VAD) review about human PUM2, based on 8 published articles (read how Labome selects the articles), using PUM2 antibody in all methods. It is aimed to help Labome visitors find the most suited PUM2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PUM2 synonym: PUMH2; PUML2; pumilio homolog 2; pumilio-2

Abcam
rabbit monoclonal (EPR3813)
  • western blot; human; 1:4000; loading ...; fig 7s3a
  • western blot; mouse; 1:4000; loading ...; fig 7s1b
Abcam PUM2 antibody (Abcam, ab92390) was used in western blot on human samples at 1:4000 (fig 7s3a) and in western blot on mouse samples at 1:4000 (fig 7s1b). elife (2019) ncbi
rabbit monoclonal (EPR3813)
  • western blot; human; loading ...; fig 8a
Abcam PUM2 antibody (Abcam, 92390) was used in western blot on human samples (fig 8a). Proc Natl Acad Sci U S A (2017) ncbi
rabbit monoclonal (EPR3813)
  • western blot; mouse; 1:1000; loading ...; fig 1a
Abcam PUM2 antibody (Abcam, ab92390) was used in western blot on mouse samples at 1:1000 (fig 1a). Biol Reprod (2016) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human; 1:1000; fig 3
  • western blot; human; fig 3
Bethyl PUM2 antibody (Bethyl, A300-202A) was used in RNA immunoprecipitation on human samples at 1:1000 (fig 3) and in western blot on human samples (fig 3). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 3
Bethyl PUM2 antibody (Bethyl Labs, A300-202A) was used in western blot on human samples at 1:1000 (fig 3). BMC Mol Biol (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl PUM2 antibody (Bethyl, A300-202A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 7b
Bethyl PUM2 antibody (Bethyl, A300-202A) was used in western blot on human samples (fig 7b). Mol Cell Biol (2015) ncbi
rabbit polyclonal
  • western blot; human; fig s3
  • western blot; mouse; fig s9
Bethyl PUM2 antibody (Bethyl, A300-202A) was used in western blot on human samples (fig s3) and in western blot on mouse samples (fig s9). EMBO J (2014) ncbi
Articles Reviewed
  1. Kopp F, Elguindy M, Yalvac M, Zhang H, Chen B, Gillett F, et al. PUMILIO hyperactivity drives premature aging of Norad-deficient mice. elife. 2019;8: pubmed publisher
  2. Liu Y, Qu L, Liu Y, Roizman B, Zhou G. PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2. Proc Natl Acad Sci U S A. 2017;114:E6902-E6911 pubmed publisher
  3. Tichon A, Gil N, Lubelsky Y, Havkin Solomon T, Lemze D, Itzkovitz S, et al. A conserved abundant cytoplasmic long noncoding RNA modulates repression by Pumilio proteins in human cells. Nat Commun. 2016;7:12209 pubmed publisher
  4. Mak W, Fang C, Holden T, Dratver M, Lin H. An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline. Biol Reprod. 2016;94:134 pubmed publisher
  5. Estep J, Sternburg E, Sanchez G, Karginov F. Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection. BMC Mol Biol. 2016;17:9 pubmed publisher
  6. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  7. Miles W, Lepesant J, Bourdeaux J, Texier M, Kerenyi M, Nakakido M, et al. The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback Loop. Mol Cell Biol. 2015;35:4199-211 pubmed publisher
  8. Miles W, Korenjak M, Griffiths L, Dyer M, Provero P, Dyson N. Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells. EMBO J. 2014;33:2201-15 pubmed publisher