This is a Validated Antibody Database (VAD) review about human PU 1, based on 28 published articles (read how Labome selects the articles), using PU 1 antibody in all methods. It is aimed to help Labome visitors find the most suited PU 1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PU 1 synonym: OF; PU.1; SFPI1; SPI-1; SPI-A

Santa Cruz Biotechnology
  • ChIP-Seq; mouse; fig 6
Santa Cruz Biotechnology PU 1 antibody (Santa Cruz, sc-352x) was used in ChIP-Seq on mouse samples (fig 6). elife (2016) ncbi
  • immunocytochemistry; mouse; 8 ug/ml; loading ...; fig 3b
In order to perform live imaging for continuous single-cell long-term quantification of the transcription factors GATA1 and PU.1 and study how these factors influence early myeloid lineage choice, Santa Cruz Biotechnology PU 1 antibody (Santa Cruz, T-21) was used in immunocytochemistry on mouse samples at 8 ug/ml (fig 3b). Nature (2016) ncbi
  • chromatin immunoprecipitation; mouse; loading ...; fig 5b
  • western blot; human; loading ...; fig s1b
Santa Cruz Biotechnology PU 1 antibody (Santa Cruz, sc352) was used in chromatin immunoprecipitation on mouse samples (fig 5b) and in western blot on human samples (fig s1b). PLoS ONE (2016) ncbi
mouse monoclonal (A-7)
  • western blot; human; 1:1000; fig 4
Santa Cruz Biotechnology PU 1 antibody (santa Cruz, sc-365208) was used in western blot on human samples at 1:1000 (fig 4). Oncotarget (2016) ncbi
  • chromatin immunoprecipitation; mouse; fig 2
  • western blot; mouse; fig 1
Santa Cruz Biotechnology PU 1 antibody (Santa Cruz, sc352x) was used in chromatin immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples (fig 1). Leukemia (2016) ncbi
Abcam
mouse monoclonal
  • immunocytochemistry; human; 1:200; fig s3c
Abcam PU 1 antibody (Abcam, ab88082) was used in immunocytochemistry on human samples at 1:200 (fig s3c). Mol Psychiatry (2017) ncbi
domestic rabbit monoclonal (EPR3158Y)
  • western blot; human; loading ...; fig 5b
In order to examine cellular changes during histone deacetylases inhibitor-induced differentiation, Abcam PU 1 antibody (Abcam, ab76543) was used in western blot on human samples (fig 5b). Epigenetics Chromatin (2016) ncbi
domestic rabbit monoclonal (EPR3158Y)
  • immunohistochemistry - paraffin section; human; fig 4
Abcam PU 1 antibody (Abcam, ab76543) was used in immunohistochemistry - paraffin section on human samples (fig 4). Clin Cancer Res (2016) ncbi
mouse monoclonal
  • western blot; mouse
Abcam PU 1 antibody (Abcam, ab88082) was used in western blot on mouse samples . Leukemia (2014) ncbi
Invitrogen
domestic rabbit polyclonal
In order to study the relationship between TAM receptor-mediated proinflammatory cytokine production and chronic NOD2 stimulation in the intestine, Invitrogen PU 1 antibody (Thermo Scientific, PA5-17505) was used . J Immunol (2015) ncbi
domestic rabbit monoclonal (E.388.3)
  • immunohistochemistry - frozen section; human; loading ...; fig 2a
In order to study the role of TH9 cells in ulcerative colitis, Invitrogen PU 1 antibody (Thermo Scientific, E.388.3) was used in immunohistochemistry - frozen section on human samples (fig 2a). Nat Immunol (2014) ncbi
LifeSpan Biosciences
domestic rabbit polyclonal
  • reverse phase protein lysate microarray; mouse; 1:500; fig 4
  • western blot; mouse
In order to elucidate a 7-month cigarette smoke inhalation study in C57BL/6 mice through system biology analysis, LifeSpan Biosciences PU 1 antibody (LifeSpan BioSciences, LSC30679) was used in reverse phase protein lysate microarray on mouse samples at 1:500 (fig 4) and in western blot on mouse samples . Sci Data (2016) ncbi
BioLegend
mouse monoclonal (7C6B05)
  • flow cytometry; human; loading ...; fig s7
BioLegend PU 1 antibody (Biolegend, 7C6B05) was used in flow cytometry on human samples (fig s7). Proc Natl Acad Sci U S A (2016) ncbi
Abnova
mouse monoclonal (2G1)
  • western blot; human; loading ...; fig 4
Abnova PU 1 antibody (Abnova, H00006688-M02) was used in western blot on human samples (fig 4). Cell Host Microbe (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3f
Cell Signaling Technology PU 1 antibody (Cell Signaling, 2266) was used in western blot on mouse samples at 1:1000 (fig 3f). J Immunother Cancer (2021) ncbi
domestic rabbit monoclonal (9G7)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 3b
Cell Signaling Technology PU 1 antibody (Cell Signaling, 9G7) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 3b). Anal Cell Pathol (Amst) (2020) ncbi
domestic rabbit polyclonal
  • flow cytometry; mouse; 1:1000; loading ...; fig s3b
  • immunohistochemistry; mouse; 1:500; loading ...; fig 1c
Cell Signaling Technology PU 1 antibody (CST, 2266) was used in flow cytometry on mouse samples at 1:1000 (fig s3b) and in immunohistochemistry on mouse samples at 1:500 (fig 1c). Nat Commun (2020) ncbi
domestic rabbit monoclonal (9G7)
  • ChIP-Seq; human; 1-2 ug / 500 ul chr; loading ...; fig 4c
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Cell Signaling Technology PU 1 antibody (Cell Signaling, 2258) was used in ChIP-Seq on human samples at 1-2 ug / 500 ul chr (fig 4c) and in chromatin immunoprecipitation on human samples (fig 4c). Cell Rep (2019) ncbi
domestic rabbit monoclonal (9G7)
  • other; mouse; fig s1d
Cell Signaling Technology PU 1 antibody (Cell Signaling Technology, 2240) was used in other on mouse samples (fig s1d). Immunity (2019) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; mouse; loading ...; fig 4h
Cell Signaling Technology PU 1 antibody (Cell Signaling, 2258 s) was used in western blot on mouse samples (fig 4h). elife (2018) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; human; loading ...; fig 9h
Cell Signaling Technology PU 1 antibody (Cell Signaling, 2258) was used in western blot on human samples (fig 9h). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; human; fig 5e
Cell Signaling Technology PU 1 antibody (Cell Signaling, 2258S) was used in western blot on human samples (fig 5e). Cell (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; fig 6
Cell Signaling Technology PU 1 antibody (Cell Signalling, 2266) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 6). Nat Commun (2016) ncbi
domestic rabbit monoclonal (9G7)
  • chromatin immunoprecipitation; human; loading ...; fig 2c
Cell Signaling Technology PU 1 antibody (Cell Signaling, 2258) was used in chromatin immunoprecipitation on human samples (fig 2c). J Hematol Oncol (2016) ncbi
domestic rabbit monoclonal (9G7)
  • immunocytochemistry; human; loading ...; fig s2a
Cell Signaling Technology PU 1 antibody (Cell Signaling Technology, 2258) was used in immunocytochemistry on human samples (fig s2a). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; mouse; 1:1000; fig 6
Cell Signaling Technology PU 1 antibody (Cell Signaling, 2258) was used in western blot on mouse samples at 1:1000 (fig 6). Nat Commun (2015) ncbi
domestic rabbit monoclonal (9G7)
  • western blot; human
In order to study the molecular signaling responsible for retinoic acid-induced differentiation, Cell Signaling Technology PU 1 antibody (Cell Signaling, 2258) was used in western blot on human samples . PLoS ONE (2015) ncbi
domestic rabbit monoclonal (9G7)
  • immunohistochemistry - free floating section; mouse; 1:400; tbl 1
Cell Signaling Technology PU 1 antibody (Cell signalling, 2258S) was used in immunohistochemistry - free floating section on mouse samples at 1:400 (tbl 1). Brain Behav Immun (2015) ncbi
BD Biosciences
mouse monoclonal (G148-74)
  • immunoprecipitation; human; fig 5
BD Biosciences PU 1 antibody (BD Biosciences, 554268) was used in immunoprecipitation on human samples (fig 5). Biomed Res Int (2016) ncbi
Articles Reviewed
  1. Schanz O, Cornez I, Yajnanarayana S, David F, Peer S, Gruber T, et al. Tumor rejection in Cblb -/- mice depends on IL-9 and Th9 cells. J Immunother Cancer. 2021;9: pubmed publisher
  2. Kovaleva O, Rashidova M, Samoilova D, Podlesnaya P, Mochalnikova V, Gratchev A. Immunosuppressive Phenotype of Esophagus Tumors Stroma. Anal Cell Pathol (Amst). 2020;2020:5424780 pubmed publisher
  3. Yu X, Liu H, Hamel K, Morvan M, Yu S, Leff J, et al. Dorsal root ganglion macrophages contribute to both the initiation and persistence of neuropathic pain. Nat Commun. 2020;11:264 pubmed publisher
  4. Senigl F, Maman Y, Dinesh R, Alinikula J, Seth R, Pecnova L, et al. Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation. Cell Rep. 2019;29:3902-3915.e8 pubmed publisher
  5. Chopin M, Lun A, Zhan Y, Schreuder J, Coughlan H, D Amico A, et al. Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT. Immunity. 2019;50:77-90.e5 pubmed publisher
  6. Zhu H, Zhang L, Wu Y, Dong B, Guo W, Wang M, et al. T-ALL leukemia stem cell 'stemness' is epigenetically controlled by the master regulator SPI1. elife. 2018;7: pubmed publisher
  7. Chennupati V, Veiga D, Maslowski K, Andina N, Tardivel A, Yu E, et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest. 2018;128:1597-1614 pubmed publisher
  8. Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  9. Hoffmann H, Schneider W, Blomen V, Scull M, Hovnanian A, Brummelkamp T, et al. Diverse Viruses Require the Calcium Transporter SPCA1 for Maturation and Spread. Cell Host Microbe. 2017;22:460-470.e5 pubmed publisher
  10. Sellgren C, Sheridan S, Gracias J, Xuan D, Fu T, Perlis R. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry. 2017;22:170-177 pubmed publisher
  11. Peters C, Häsler R, Wesch D, Kabelitz D. Human Vδ2 T cells are a major source of interleukin-9. Proc Natl Acad Sci U S A. 2016;113:12520-12525 pubmed
  12. Alivernini S, Kurowska Stolarska M, Tolusso B, Benvenuto R, Elmesmari A, Canestri S, et al. MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis. Nat Commun. 2016;7:12970 pubmed publisher
  13. Eichenfield D, Troutman T, Link V, Lam M, Cho H, Gosselin D, et al. Tissue damage drives co-localization of NF-?B, Smad3, and Nrf2 to direct Rev-erb sensitive wound repair in mouse macrophages. elife. 2016;5: pubmed publisher
  14. Hoppe P, Schwarzfischer M, Loeffler D, Kokkaliaris K, Hilsenbeck O, Moritz N, et al. Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios. Nature. 2016;535:299-302 pubmed publisher
  15. Wei S, Zhao M, Wang X, Li Y, Wang K. PU.1 controls the expression of long noncoding RNA HOTAIRM1 during granulocytic differentiation. J Hematol Oncol. 2016;9:44 pubmed publisher
  16. Frank C, Manandhar D, Gordân R, Crawford G. HDAC inhibitors cause site-specific chromatin remodeling at PU.1-bound enhancers in K562 cells. Epigenetics Chromatin. 2016;9:15 pubmed publisher
  17. Burda P, Vargova J, Curik N, Salek C, Papadopoulos G, Strouboulis J, et al. GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia. PLoS ONE. 2016;11:e0152234 pubmed publisher
  18. Rogers J, Owens K, Kurkewich J, Klopfenstein N, Iyer S, Simon M, et al. E2A Antagonizes PU.1 Activity through Inhibition of DNA Binding. Biomed Res Int. 2016;2016:3983686 pubmed publisher
  19. Vieyra Garcia P, Wei T, Naym D, Fredholm S, Fink Puches R, Cerroni L, et al. STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides. Clin Cancer Res. 2016;22:3328-39 pubmed publisher
  20. Ansari S, Baumer K, Boué S, Dijon S, Dulize R, Ekroos K, et al. Comprehensive systems biology analysis of a 7-month cigarette smoke inhalation study in C57BL/6 mice. Sci Data. 2016;3:150077 pubmed publisher
  21. Song G, Shi L, Guo Y, Yu L, Wang L, Zhang X, et al. A novel PAD4/SOX4/PU.1 signaling pathway is involved in the committed differentiation of acute promyelocytic leukemia cells into granulocytic cells. Oncotarget. 2016;7:3144-57 pubmed publisher
  22. Xiao X, Shi X, Fan Y, Zhang X, Wu M, Lan P, et al. GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation. Nat Commun. 2015;6:8266 pubmed publisher
  23. Bunaciu R, Jensen H, Macdonald R, Latocha D, Varner J, Yen A. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS ONE. 2015;10:e0135668 pubmed publisher
  24. Sive J, Basilico S, Hannah R, Kinston S, Calero Nieto F, Göttgens B. Genome-scale definition of the transcriptional programme associated with compromised PU.1 activity in acute myeloid leukaemia. Leukemia. 2016;30:14-23 pubmed publisher
  25. Zheng S, Hedl M, Abraham C. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages. J Immunol. 2015;194:1928-37 pubmed publisher
  26. Almolda B, de Labra C, Barrera I, Gruart A, Delgado Garcia J, Villacampa N, et al. Alterations in microglial phenotype and hippocampal neuronal function in transgenic mice with astrocyte-targeted production of interleukin-10. Brain Behav Immun. 2015;45:80-97 pubmed publisher
  27. Gerlach K, Hwang Y, Nikolaev A, Atreya R, Dornhoff H, Steiner S, et al. TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells. Nat Immunol. 2014;15:676-86 pubmed publisher
  28. Zhou J, Wu J, Li B, Liu D, Yu J, Yan X, et al. PU.1 is essential for MLL leukemia partially via crosstalk with the MEIS/HOX pathway. Leukemia. 2014;28:1436-48 pubmed publisher