This is a Validated Antibody Database (VAD) review about human PTTG1, based on 17 published articles (read how Labome selects the articles), using PTTG1 antibody in all methods. It is aimed to help Labome visitors find the most suited PTTG1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PTTG1 synonym: EAP1; HPTTG; PTTG; TUTR1

Invitrogen
domestic rabbit recombinant (19H16L48)
  • western blot; mouse; loading ...; fig 1b
Invitrogen PTTG1 antibody (Invitrogen, 700791) was used in western blot on mouse samples (fig 1b). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 5k
  • immunohistochemistry; human; loading ...; fig 6g
  • western blot; human; loading ...; fig s5b
  • western blot; mouse; loading ...; fig 6a
In order to show that pituitary tumor transforming gene 1 expression is upregulated in an mammalian/mechanistic target of rapamycin complex 1-dependent manner, Invitrogen PTTG1 antibody (Invitrogen, 34-1500) was used in western blot on rat samples (fig 5k), in immunohistochemistry on human samples (fig 6g), in western blot on human samples (fig s5b) and in western blot on mouse samples (fig 6a). Oncogene (2017) ncbi
domestic rabbit recombinant (19H16L48)
  • western blot; human; loading ...; fig s1i
In order to describe the structure of the human anaphase-promoting complex interacting with the mitotic checkpoint complex, Invitrogen PTTG1 antibody (Invitrogen, 700791) was used in western blot on human samples (fig s1i). Nature (2016) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; fig 3
In order to characterize novel pancreas-targeting activity through the binding of Sec 61 by Apratoxin A, Invitrogen PTTG1 antibody (Life Technologies, 34-1500) was used in immunoprecipitation on human samples (fig 3). Mol Cancer Ther (2016) ncbi
domestic rabbit recombinant (19H16L48)
  • western blot; human
In order to describe the anti-cancer effects of KPYB10602, Invitrogen PTTG1 antibody (Invitrogen, 19H16L48) was used in western blot on human samples . Biochem Biophys Res Commun (2015) ncbi
domestic rabbit polyclonal
In order to study the effect of MASTL on cyclin B1 degradation and its mechanism, Invitrogen PTTG1 antibody (Zymed, 34-1500) was used . Biol Open (2015) ncbi
domestic rabbit polyclonal
In order to study CDC26 phosphorylation and its effect on the tetratricopeptide repeat subcomplex of APC/C, Invitrogen PTTG1 antibody (Invitrogen, 34-1500) was used . PLoS ONE (2015) ncbi
domestic rabbit polyclonal
In order to explore how Nek2 isoform A affects anaphase-promoting complex/cyclosome containing Cdc20, Invitrogen PTTG1 antibody (Zymed, 34-1500) was used . J Cell Sci (2015) ncbi
Abcam
domestic rabbit monoclonal (EPR3240)
  • western blot; human; 1:1000; loading ...; fig 4c
Abcam PTTG1 antibody (Abcam, ab79546) was used in western blot on human samples at 1:1000 (fig 4c). Front Pharmacol (2021) ncbi
domestic rabbit monoclonal (EPR3240)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 2a
Abcam PTTG1 antibody (Abcam, EPR3240) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 2a). Front Endocrinol (Lausanne) (2019) ncbi
domestic rabbit monoclonal (EPR3240)
  • immunoprecipitation; human; loading ...; fig 6b
  • western blot; human; loading ...; fig 5c, 5d
Abcam PTTG1 antibody (abcam, ab79546) was used in immunoprecipitation on human samples (fig 6b) and in western blot on human samples (fig 5c, 5d). Oncotarget (2017) ncbi
domestic rabbit monoclonal (EPR3240)
  • western blot; human; loading ...; fig s4
Abcam PTTG1 antibody (epitomics, 2603-1) was used in western blot on human samples (fig s4). Oncotarget (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • immunocytochemistry; mouse; 1:50; fig 4
Santa Cruz Biotechnology PTTG1 antibody (Santa Cruz, sc-398471) was used in immunocytochemistry on mouse samples at 1:50 (fig 4). Sci Rep (2016) ncbi
mouse monoclonal (DCS-280)
  • western blot; human; fig 1
Santa Cruz Biotechnology PTTG1 antibody (Santa Cruz, Sc-56207) was used in western blot on human samples (fig 1). J Cell Biochem (2015) ncbi
mouse monoclonal (DCS-280)
  • western blot; human
In order to characterize securin isoforms and their role in separase regulation, Santa Cruz Biotechnology PTTG1 antibody (Santa Cruz, sc-56207) was used in western blot on human samples . Mol Cell Biol (2013) ncbi
MBL International
monoclonal
  • western blot; human; 1:2000; fig 2
MBL International PTTG1 antibody (MBL, K0090-3) was used in western blot on human samples at 1:2000 (fig 2). Nat Commun (2016) ncbi
monoclonal
  • western blot; human; 1:500; fig 5
MBL International PTTG1 antibody (MBL, K0090-3) was used in western blot on human samples at 1:500 (fig 5). Nat Commun (2016) ncbi
Articles Reviewed
  1. Hong H, Jin Z, Qian T, Xu X, Zhu X, Fei Q, et al. Falcarindiol Enhances Cisplatin Chemosensitivity of Hepatocellular Carcinoma via Down-Regulating the STAT3-Modulated PTTG1 Pathway. Front Pharmacol. 2021;12:656697 pubmed publisher
  2. Grande G, Milardi D, Martini M, Cenci T, Gulino G, Mancini F, et al. Protein Expression of PTTG-1, OCT-4, and KLF-4 in Seminoma: A Pilot Study. Front Endocrinol (Lausanne). 2019;10:619 pubmed publisher
  3. Zou Y, Qiu G, Jiang L, Cai Z, Sun W, Hu H, et al. Overexpression of ubiquitin specific proteases 44 promotes the malignancy of glioma by stabilizing tumor-promoter securin. Oncotarget. 2017;8:58231-58246 pubmed publisher
  4. Read M, Fong J, Modasia B, Fletcher A, Imruetaicharoenchoke W, Thompson R, et al. Elevated PTTG and PBF predicts poor patient outcome and modulates DNA damage response genes in thyroid cancer. Oncogene. 2017;36:5296-5308 pubmed publisher
  5. Chen R, Duan J, Li L, Ma Q, Sun Q, Ma J, et al. mTOR promotes pituitary tumor development through activation of PTTG1. Oncogene. 2017;36:979-988 pubmed publisher
  6. Alfieri C, Chang L, Zhang Z, Yang J, Maslen S, Skehel M, et al. Molecular basis of APC/C regulation by the spindle assembly checkpoint. Nature. 2016;536:431-436 pubmed publisher
  7. Yuan Y, Ren Y, Yuan P, Yan L, Qiao J. TRAIP is involved in chromosome alignment and SAC regulation in mouse oocyte meiosis. Sci Rep. 2016;6:29735 pubmed publisher
  8. Huang K, Chen Z, Jiang Y, Akare S, Kolber Simonds D, Condon K, et al. Apratoxin A Shows Novel Pancreas-Targeting Activity through the Binding of Sec 61. Mol Cancer Ther. 2016;15:1208-16 pubmed publisher
  9. Iimori M, Watanabe S, Kiyonari S, Matsuoka K, Sakasai R, Saeki H, et al. Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nat Commun. 2016;7:11117 pubmed publisher
  10. Hein J, Nilsson J. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nat Commun. 2016;7:10975 pubmed publisher
  11. Zhang P, Zhang Y, Gao K, Wang Y, Jin X, Wei Y, et al. ASPP1/2-PP1 complexes are required for chromosome segregation and kinetochore-microtubule attachments. Oncotarget. 2015;6:41550-65 pubmed publisher
  12. Takenaga M, Yamamoto Y, Takeuchi T, Ohta Y, Tokura Y, Hamaguchi A, et al. Potential new chemotherapy strategy for human ovarian carcinoma with a novel KSP inhibitor. Biochem Biophys Res Commun. 2015;463:222-8 pubmed publisher
  13. Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  14. Masuda K, Chiyoda T, Sugiyama N, Segura Cabrera A, Kabe Y, Ueki A, et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS ONE. 2015;10:e0118662 pubmed publisher
  15. Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher
  16. Jeong H, Gil N, Lee H, Cho S, Kim K, Chun K, et al. Timely Degradation of Wip1 Phosphatase by APC/C Activator Protein Cdh1 is Necessary for Normal Mitotic Progression. J Cell Biochem. 2015;116:1602-12 pubmed publisher
  17. Han X, Poon R. Critical differences between isoforms of securin reveal mechanisms of separase regulation. Mol Cell Biol. 2013;33:3400-15 pubmed publisher