This is a Validated Antibody Database (VAD) review about human PTCH1, based on 17 published articles (read how Labome selects the articles), using PTCH1 antibody in all methods. It is aimed to help Labome visitors find the most suited PTCH1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PTCH1 synonym: BCNS; NBCCS; PTC; PTC1; PTCH; protein patched homolog 1

Abcam
rabbit polyclonal
  • western blot; human; loading ...; fig 6a
Abcam PTCH1 antibody (Abcam, ab53715) was used in western blot on human samples (fig 6a). Clin Cancer Res (2018) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:1000; fig 3,5,6
In order to evaluate a rat ACL reconstruction model and the role of indian hedgehog signaling and the role of graft tension in tendon-to-bone healing, Abcam PTCH1 antibody (Abcam, ab53715) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000 (fig 3,5,6). J Orthop Res (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100
Abcam PTCH1 antibody (Abcam, ab53715) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Cell Death Dis (2015) ncbi
goat polyclonal
  • western blot; human
Abcam PTCH1 antibody (Abcam, ab51983) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200
Abcam PTCH1 antibody (Abcam, ab53715) was used in immunohistochemistry - paraffin section on human samples at 1:200. Korean J Pathol (2013) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:100
  • western blot; rat; 1:200
Abcam PTCH1 antibody (Abcam, ab53715) was used in immunohistochemistry on rat samples at 1:100 and in western blot on rat samples at 1:200. J Comp Neurol (2011) ncbi
R&D Systems
rat monoclonal (413220)
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1b
R&D Systems PTCH1 antibody (R&D Systems, MAB41051) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 1b). Proc Natl Acad Sci U S A (2017) ncbi
rat monoclonal (413220)
  • western blot; mouse; 1:500; fig 5
In order to study postnatal testicular development in Gpr37-null mutant mice, R&D Systems PTCH1 antibody (R&D System, 413220) was used in western blot on mouse samples at 1:500 (fig 5). FASEB J (2015) ncbi
Novus Biologicals
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s5
Novus Biologicals PTCH1 antibody (Novus Biologicals, NB200-118) was used in immunohistochemistry - paraffin section on mouse samples (fig s5). PLoS Genet (2016) ncbi
LifeSpan Biosciences
goat polyclonal
  • ELISA; human
  • western blot; human; 1:1000; fig 1
LifeSpan Biosciences PTCH1 antibody (LS-Bio, LS-B3939) was used in ELISA on human samples and in western blot on human samples at 1:1000 (fig 1). J Exp Med (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; mouse
Proteintech Group PTCH1 antibody (Protein Tech, 17520-1-AP) was used in western blot on mouse samples . Mol Cancer Res (2013) ncbi
Cell Signaling Technology
rabbit monoclonal (C53A3)
  • immunoprecipitation; human; loading ...; fig 1f
Cell Signaling Technology PTCH1 antibody (Cell Signaling, 2468) was used in immunoprecipitation on human samples (fig 1f). Oncogene (2018) ncbi
rabbit monoclonal (C53A3)
  • western blot; human; loading ...; fig 5
Cell Signaling Technology PTCH1 antibody (Cell Signaling, 2468) was used in western blot on human samples (fig 5). Mol Clin Oncol (2016) ncbi
rabbit monoclonal (C53A3)
  • western blot; human; 1:1000; fig 6
Cell Signaling Technology PTCH1 antibody (Cell Signaling, 2468) was used in western blot on human samples at 1:1000 (fig 6). Mol Med Rep (2016) ncbi
rabbit monoclonal (C53A3)
  • western blot; human; 1:1000; fig 1
Cell Signaling Technology PTCH1 antibody (Cell Signaling, 2468s) was used in western blot on human samples at 1:1000 (fig 1). Int J Mol Med (2015) ncbi
Sigma-Aldrich
goat polyclonal
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Sigma-Aldrich PTCH1 antibody (SIGMA, polyclonal/SAB2500835) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (1G2)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Sigma-Aldrich PTCH1 antibody (SIGMA, 1G2) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; fig 2
EMD Millipore PTCH1 antibody (Millipore, 06-1102) was used in western blot on human samples (fig 2). Cell Signal (2015) ncbi
Articles Reviewed
  1. Li N, Truong S, Nouri M, Moore J, Al Nakouzi N, Lubik A, et al. Non-canonical activation of hedgehog in prostate cancer cells mediated by the interaction of transcriptionally active androgen receptor proteins with Gli3. Oncogene. 2018;37:2313-2325 pubmed publisher
  2. Maurya D, Bohm S, Alenius M. Hedgehog signaling regulates ciliary localization of mouse odorant receptors. Proc Natl Acad Sci U S A. 2017;114:E9386-E9394 pubmed publisher
  3. Zhao Z, Jia Q, Wu M, Xie X, Wang Y, Song G, et al. Degalactotigonin, a Natural Compound from Solanum nigrum L., Inhibits Growth and Metastasis of Osteosarcoma through GSK3β Inactivation-Mediated Repression of the Hedgehog/Gli1 Pathway. Clin Cancer Res. 2018;24:130-144 pubmed publisher
  4. Galoian K, Luo S, Qureshi A, Patel P, Price R, Morse A, et al. Effect of cytostatic proline rich polypeptide-1 on tumor suppressors of inflammation pathway signaling in chondrosarcoma. Mol Clin Oncol. 2016;5:618-624 pubmed
  5. Wang W, Song B, Anbarchian T, Shirazyan A, Sadik J, Lyons K. Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate. PLoS Genet. 2016;12:e1006352 pubmed publisher
  6. Wang H, Ning Z, Li Y, Zhu X, Meng Z. Bufalin suppresses cancer stem-like cells in gemcitabine-resistant pancreatic cancer cells via Hedgehog signaling. Mol Med Rep. 2016;14:1907-14 pubmed publisher
  7. Klein C, Zwick A, Kissel S, Forster C, Pfeifer D, Follo M, et al. Ptch2 loss drives myeloproliferation and myeloproliferative neoplasm progression. J Exp Med. 2016;213:273-90 pubmed publisher
  8. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  9. Carbone A, Carballo C, Ma R, Wang H, Deng X, Dahia C, et al. Indian hedgehog signaling and the role of graft tension in tendon-to-bone healing: Evaluation in a rat ACL reconstruction model. J Orthop Res. 2016;34:641-9 pubmed publisher
  10. Fan C, Wang Y, Liu Z, Sun Y, Wang X, Wei G, et al. Metformin exerts anticancer effects through the inhibition of the Sonic hedgehog signaling pathway in breast cancer. Int J Mol Med. 2015;36:204-14 pubmed publisher
  11. Pan Y, Gong Y, Ruan H, Pan L, Wu X, Tang C, et al. Sonic hedgehog through Gli2 and Gli3 is required for the proper development of placental labyrinth. Cell Death Dis. 2015;6:e1653 pubmed publisher
  12. La Sala G, Marazziti D, Di Pietro C, Golini E, Matteoni R, Tocchini Valentini G. Modulation of Dhh signaling and altered Sertoli cell function in mice lacking the GPR37-prosaposin receptor. FASEB J. 2015;29:2059-69 pubmed publisher
  13. Tang C, Pan Y, Luo H, Xiong W, Zhu H, Ruan H, et al. Hedgehog signaling stimulates the conversion of cholesterol to steroids. Cell Signal. 2015;27:487-97 pubmed publisher
  14. Konitsiotis A, Chang S, Jovanovic B, Ciepla P, Masumoto N, Palmer C, et al. Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells. PLoS ONE. 2014;9:e89899 pubmed publisher
  15. Gurung B, Feng Z, Hua X. Menin directly represses Gli1 expression independent of canonical Hedgehog signaling. Mol Cancer Res. 2013;11:1215-22 pubmed publisher
  16. Im S, Choi H, Yoo C, Jung J, Jeon Y, Suh Y, et al. Hedgehog related protein expression in breast cancer: gli-2 is associated with poor overall survival. Korean J Pathol. 2013;47:116-23 pubmed publisher
  17. Petralia R, Schwartz C, Wang Y, Mattson M, Yao P. Subcellular localization of Patched and Smoothened, the receptors for Sonic hedgehog signaling, in the hippocampal neuron. J Comp Neurol. 2011;519:3684-99 pubmed publisher