This is a Validated Antibody Database (VAD) review about human PSIP1, based on 14 published articles (read how Labome selects the articles), using PSIP1 antibody in all methods. It is aimed to help Labome visitors find the most suited PSIP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PSIP1 synonym: DFS70; LEDGF; PAIP; PSIP2; p52; p75; PC4 and SFRS1-interacting protein; CLL-associated antigen KW-7; dense fine speckles 70 kDa protein; lens epithelium-derived growth factor; transcriptional coactivator p52/p75

Knockout validation
Bethyl
rabbit polyclonal
  • western blot knockout validation; human; fig 1
Bethyl PSIP1 antibody (Bethyl Laboratories, A300-848A) was used in western blot knockout validation on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (C57G11)
  • western blot knockout validation; human; fig 2
Cell Signaling Technology PSIP1 antibody (Cell Signaling, C57G11) was used in western blot knockout validation on human samples (fig 2). Antimicrob Agents Chemother (2014) ncbi
BD Biosciences
mouse monoclonal (26/LEDGF)
  • western blot knockout validation; human; fig 2
BD Biosciences PSIP1 antibody (BD, 611714) was used in western blot knockout validation on human samples (fig 2). Antimicrob Agents Chemother (2014) ncbi
Bethyl
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 5
  • immunoprecipitation; mouse; fig 1
  • western blot; mouse; 1:1500; fig 1
Bethyl PSIP1 antibody (Bethyl, A300-847A) was used in chromatin immunoprecipitation on mouse samples (fig 5), in immunoprecipitation on mouse samples (fig 1) and in western blot on mouse samples at 1:1500 (fig 1). PLoS Genet (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl PSIP1 antibody (Bethyl, A300-848A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; human; fig 1
Bethyl PSIP1 antibody (Bethyl Laboratories, A300-848A) was used in western blot knockout validation on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 1b
  • western blot; human; fig 1j
Bethyl PSIP1 antibody (Bethyl Laboratories, A300-848) was used in immunocytochemistry on human samples (fig 1b) and in western blot on human samples (fig 1j). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Bethyl PSIP1 antibody (Bethyl laboratories, A300-848A) was used in western blot on human samples (fig 1). Retrovirology (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig s6
Bethyl PSIP1 antibody (Bethyl, A300-847A) was used in western blot on human samples at 1:1000 (fig s6). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig s6
Bethyl PSIP1 antibody (Bethyl, A300-847A) was used in immunoprecipitation on human samples (fig s6). Cell Host Microbe (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Bethyl PSIP1 antibody (Bethyl, A300-848A) was used in western blot on human samples at 1:1000. J Virol (2014) ncbi
rabbit polyclonal
  • immunoprecipitation; rat; fig 5
In order to study the induction of the breast cancer susceptibility associated gene FBXO10 by cellular stress, Bethyl PSIP1 antibody (Bethyl, A300-848A) was used in immunoprecipitation on rat samples (fig 5). Mol Carcinog (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (C57G11)
  • western blot; human; loading ...; fig s1f
Cell Signaling Technology PSIP1 antibody (Cell Signaling, 2088) was used in western blot on human samples (fig s1f). Genes Dev (2019) ncbi
rabbit monoclonal (C57G11)
  • western blot knockout validation; human; fig 2
Cell Signaling Technology PSIP1 antibody (Cell Signaling, C57G11) was used in western blot knockout validation on human samples (fig 2). Antimicrob Agents Chemother (2014) ncbi
BD Biosciences
mouse monoclonal (26/LEDGF)
  • immunocytochemistry; human; fig 1
  • western blot; human; 1:500; fig 1
BD Biosciences PSIP1 antibody (BD Biosciences, 611,714) was used in immunocytochemistry on human samples (fig 1) and in western blot on human samples at 1:500 (fig 1). J Mol Biol (2016) ncbi
mouse monoclonal (26/LEDGF)
  • western blot; human; fig 4
BD Biosciences PSIP1 antibody (BD Biosciences, 611714) was used in western blot on human samples (fig 4). Oncogene (2015) ncbi
mouse monoclonal (26/LEDGF)
  • western blot; human; 1:500
BD Biosciences PSIP1 antibody (BD, 611714) was used in western blot on human samples at 1:500. J Virol (2014) ncbi
mouse monoclonal (26/LEDGF)
  • western blot knockout validation; human; fig 2
BD Biosciences PSIP1 antibody (BD, 611714) was used in western blot knockout validation on human samples (fig 2). Antimicrob Agents Chemother (2014) ncbi
mouse monoclonal (26/LEDGF)
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:2000
BD Biosciences PSIP1 antibody (BD, 611714) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:2000. PLoS ONE (2013) ncbi
Articles Reviewed
  1. Zhao Z, Wang L, Volk A, Birch N, Stoltz K, Bartom E, et al. Regulation of MLL/COMPASS stability through its proteolytic cleavage by taspase1 as a possible approach for clinical therapy of leukemia. Genes Dev. 2019;33:61-74 pubmed publisher
  2. Li R, Dong Q, Yuan X, Zeng X, Gao Y, Chiao C, et al. Misregulation of Alternative Splicing in a Mouse Model of Rett Syndrome. PLoS Genet. 2016;12:e1006129 pubmed publisher
  3. Lopez A, Kugelman J, Garcia Rivera J, Urias E, Salinas S, Fernandez Zapico M, et al. The Structure-Specific Recognition Protein 1 Associates with Lens Epithelium-Derived Growth Factor Proteins and Modulates HIV-1 Replication. J Mol Biol. 2016;428:2814-31 pubmed publisher
  4. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  5. Sowd G, Serrao E, Wang H, Wang W, Fadel H, Poeschla E, et al. A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin. Proc Natl Acad Sci U S A. 2016;113:E1054-63 pubmed publisher
  6. Baude A, Aaes T, Zhai B, Al Nakouzi N, Oo H, Daugaard M, et al. Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. Nucleic Acids Res. 2016;44:2214-26 pubmed publisher
  7. Desimmie B, Weydert C, Schrijvers R, Vets S, Demeulemeester J, Proost P, et al. HIV-1 IN/Pol recruits LEDGF/p75 into viral particles. Retrovirology. 2015;12:16 pubmed publisher
  8. Lelek M, Casartelli N, Pellin D, Rizzi E, Souque P, Severgnini M, et al. Chromatin organization at the nuclear pore favours HIV replication. Nat Commun. 2015;6:6483 pubmed publisher
  9. Kanu N, Grönroos E, Martinez P, Burrell R, Yi Goh X, Bartkova J, et al. SETD2 loss-of-function promotes renal cancer branched evolution through replication stress and impaired DNA repair. Oncogene. 2015;34:5699-708 pubmed publisher
  10. Demeulemeester J, Vets S, Schrijvers R, Madlala P, De Maeyer M, De Rijck J, et al. HIV-1 integrase variants retarget viral integration and are associated with disease progression in a chronic infection cohort. Cell Host Microbe. 2014;16:651-62 pubmed publisher
  11. Fadel H, Morrison J, Saenz D, Fuchs J, Kvaratskhelia M, Ekker S, et al. TALEN knockout of the PSIP1 gene in human cells: analyses of HIV-1 replication and allosteric integrase inhibitor mechanism. J Virol. 2014;88:9704-17 pubmed publisher
  12. Badia R, Pauls E, Riveira Munoz E, Clotet B, Este J, Ballana E. Zinc finger endonuclease targeting PSIP1 inhibits HIV-1 integration. Antimicrob Agents Chemother. 2014;58:4318-27 pubmed publisher
  13. Morchikh M, Naughtin M, Di Nunzio F, Xavier J, Charneau P, Jacob Y, et al. TOX4 and NOVA1 proteins are partners of the LEDGF PWWP domain and affect HIV-1 replication. PLoS ONE. 2013;8:e81217 pubmed publisher
  14. Xu X, Powell D, Lambring C, Puckett A, Deschenes L, Prough R, et al. Human MCS5A1 candidate breast cancer susceptibility gene FBXO10 is induced by cellular stress and correlated with lens epithelium-derived growth factor (LEDGF). Mol Carcinog. 2014;53:300-13 pubmed publisher