This is a Validated Antibody Database (VAD) review about human PRG4, based on 10 published articles (read how Labome selects the articles), using PRG4 antibody in all methods. It is aimed to help Labome visitors find the most suited PRG4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PRG4 synonym: CACP; HAPO; JCAP; MSF; SZP; proteoglycan 4; articular superficial zone protein; hemangiopoietin; lubricin; megakaryocyte stimulating factor; superficial zone proteoglycan

Abcam
rabbit polyclonal
  • western blot; equine; loading ...; fig s1b
Abcam PRG4 antibody (Abcam, ab28484) was used in western blot on equine samples (fig s1b). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:100; fig 4
Abcam PRG4 antibody (Abcam, ab28484) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 4). Int J Mol Sci (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human
In order to investigate the effect of TNF and IL-10 on synovial fibroblasts, Abcam PRG4 antibody (Abcam, Ab28484) was used in immunocytochemistry on human samples . Cell Tissue Res (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
In order to study the role of Notch signaling in murine articular cartilage and joint maintenance, Abcam PRG4 antibody (Abcam, ab28484) was used in immunohistochemistry - paraffin section on mouse samples . Arthritis Rheum (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
Abcam PRG4 antibody (Abcam, ab 28484) was used in immunohistochemistry - paraffin section on mouse samples . Sci Transl Med (2013) ncbi
Invitrogen
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 6g
In order to investigate the role of Hox genes in regional patterning and integration of the musculoskeletal system, Invitrogen PRG4 antibody (Thermo Scientific, PA3-118) was used in immunohistochemistry on mouse samples at 1:200 (fig 6g). Development (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 4a
In order to propose that lubricin binds to polymorphonuclear granulocytes via L-selectin-dependent and -independent manners, Invitrogen PRG4 antibody (Thermo Scientific, PA3-118) was used in immunohistochemistry - paraffin section on human samples (fig 4a). J Biol Chem (2012) ncbi
EMD Millipore
mouse monoclonal (9G3)
  • immunohistochemistry - frozen section; human; 1:250; loading ...; fig 6
In order to test if TGF-beta supplementation stimulates chondrocyte redifferentiation, EMD Millipore PRG4 antibody (EMD Millipore, MAB401) was used in immunohistochemistry - frozen section on human samples at 1:250 (fig 6). Tissue Eng Part A (2017) ncbi
mouse monoclonal (9G3)
  • immunohistochemistry; equine; 1:1000; fig 3a
  • ELISA; equine; 1:10,000; loading ...
  • western blot; equine; loading ...
EMD Millipore PRG4 antibody (Millipore, MABT401) was used in immunohistochemistry on equine samples at 1:1000 (fig 3a), in ELISA on equine samples at 1:10,000 and in western blot on equine samples . Osteoarthritis Cartilage (2017) ncbi
mouse monoclonal (9G3)
  • immunohistochemistry - paraffin section; equine; loading ...; fig 3b
EMD Millipore PRG4 antibody (EMD Millipore, MABT401) was used in immunohistochemistry - paraffin section on equine samples (fig 3b). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; pig; 1:500
EMD Millipore PRG4 antibody (Millipore, AB2200) was used in immunohistochemistry - paraffin section on pig samples at 1:500. J Dent Res (2015) ncbi
Articles Reviewed
  1. Bianchi V, Weber J, Waldman S, Backstein D, Kandel R. Formation of Hyaline Cartilage Tissue by Passaged Human Osteoarthritic Chondrocytes. Tissue Eng Part A. 2017;23:156-165 pubmed publisher
  2. Reesink H, Watts A, Mohammed H, Jay G, Nixon A. Lubricin/proteoglycan 4 increases in both experimental and naturally occurring equine osteoarthritis. Osteoarthritis Cartilage. 2017;25:128-137 pubmed publisher
  3. Reesink H, Bonnevie E, Liu S, Shurer C, Hollander M, Bonassar L, et al. Galectin-3 Binds to Lubricin and Reinforces the Lubricating Boundary Layer of Articular Cartilage. Sci Rep. 2016;6:25463 pubmed publisher
  4. Szychlinska M, Trovato F, Di Rosa M, Malaguarnera L, Puzzo L, Leonardi R, et al. Co-Expression and Co-Localization of Cartilage Glycoproteins CHI3L1 and Lubricin in Osteoarthritic Cartilage: Morphological, Immunohistochemical and Gene Expression Profiles. Int J Mol Sci. 2016;17:359 pubmed publisher
  5. Asakawa Tanne Y, Su S, Kunimatsu R, Hirose N, Mitsuyoshi T, Okamoto Y, et al. Effects of enzymatic degradation after loading in temporomandibular joint. J Dent Res. 2015;94:337-43 pubmed publisher
  6. Mrosewski I, Jork N, Gorte K, Conrad C, Wiegand E, Kohl B, et al. Regulation of osteoarthritis-associated key mediators by TNF? and IL-10: effects of IL-10 overexpression in human synovial fibroblasts and a synovial cell line. Cell Tissue Res. 2014;357:207-23 pubmed publisher
  7. Swinehart I, Schlientz A, Quintanilla C, Mortlock D, Wellik D. Hox11 genes are required for regional patterning and integration of muscle, tendon and bone. Development. 2013;140:4574-82 pubmed publisher
  8. Mirando A, Liu Z, Moore T, Lang A, Kohn A, Osinski A, et al. RBP-J?-dependent Notch signaling is required for murine articular cartilage and joint maintenance. Arthritis Rheum. 2013;65:2623-33 pubmed publisher
  9. Ruan M, Erez A, Guse K, Dawson B, Bertin T, Chen Y, et al. Proteoglycan 4 expression protects against the development of osteoarthritis. Sci Transl Med. 2013;5:176ra34 pubmed publisher
  10. Jin C, Ekwall A, Bylund J, Björkman L, Estrella R, Whitelock J, et al. Human synovial lubricin expresses sialyl Lewis x determinant and has L-selectin ligand activity. J Biol Chem. 2012;287:35922-33 pubmed publisher