This is a Validated Antibody Database (VAD) review about human PREP, based on 4 published articles (read how Labome selects the articles), using PREP antibody in all methods. It is aimed to help Labome visitors find the most suited PREP antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PREP synonym: PEP; prolyl endopeptidase; dJ355L5.1 (prolyl endopeptidase); post-proline cleaving enzyme; prolyl oligopeptidase

Abcam
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig 3b
  • immunocytochemistry; rat; loading ...; fig 8c
  • western blot; rat; 1:5000; loading ...; fig 2a
  • immunocytochemistry; human; loading ...; fig 9c
  • western blot; human; 1:5000; loading ...; fig 7a
Abcam PREP antibody (Abcam, ab58988) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 3b), in immunocytochemistry on rat samples (fig 8c), in western blot on rat samples at 1:5000 (fig 2a), in immunocytochemistry on human samples (fig 9c) and in western blot on human samples at 1:5000 (fig 7a). J Histochem Cytochem (2019) ncbi
rabbit polyclonal
  • western blot; mouse
In order to examine the role of prolyl endopeptidase in glucose metabolism and pancreatic function, Abcam PREP antibody (Abcam, ab58988) was used in western blot on mouse samples . Proc Natl Acad Sci U S A (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1,000
Abcam PREP antibody (Abcam, ab58988) was used in western blot on human samples at 1:1,000. Cell Mol Life Sci (2014) ncbi
R&D Systems
goat polyclonal
  • western blot; human; 1:500
In order to study the potential involvement of prolyl oligopeptidase in neurodegenerative diseases by monitoring its colocalization with pathological proteins in Parkinson's and Alzheimer's diseases, R&D Systems PREP antibody (R&D Systems Minneapolis, AF4308) was used in western blot on human samples at 1:500. Neuroscience (2013) ncbi
Articles Reviewed
  1. Venditti M, Minucci S. Subcellular Localization of Prolyl Endopeptidase During the First Wave of Rat Spermatogenesis and in Rat and Human Sperm. J Histochem Cytochem. 2019;67:229-243 pubmed publisher
  2. Kim J, Toda C, D Agostino G, Zeiss C, DiLeone R, Elsworth J, et al. Hypothalamic prolyl endopeptidase (PREP) regulates pancreatic insulin and glucagon secretion in mice. Proc Natl Acad Sci U S A. 2014;111:11876-81 pubmed publisher
  3. Justa Schuch D, Moller U, Geiss Friedlander R. The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus. Cell Mol Life Sci. 2014;71:3611-26 pubmed publisher
  4. Hannula M, Myöhänen T, Tenorio Laranga J, Mannisto P, Garcia Horsman J. Prolyl oligopeptidase colocalizes with ?-synuclein, ?-amyloid, tau protein and astroglia in the post-mortem brain samples with Parkinson's and Alzheimer's diseases. Neuroscience. 2013;242:140-50 pubmed publisher