This is a Validated Antibody Database (VAD) review about human PRAME, based on 2 published articles (read how Labome selects the articles), using PRAME antibody in all methods. It is aimed to help Labome visitors find the most suited PRAME antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PRAME synonym: CT130; MAPE; OIP-4; OIP4; melanoma antigen preferentially expressed in tumors; Opa-interacting protein OIP4; cancer/testis antigen 130; opa-interacting protein 4; preferentially expressed antigen of melanoma

Santa Cruz Biotechnology
mouse monoclonal (H-10)
  • western blot; human; loading ...; fig 1b, 4d
Santa Cruz Biotechnology PRAME antibody (Santa Cruz, sc-137188) was used in western blot on human samples (fig 1b, 4d). Cancer Lett (2017) ncbi
mouse monoclonal (H-10)
  • immunohistochemistry; human; 1:200; loading ...; tbl 1
  • western blot; human; 1:300; tbl 1
In order to research the expression and the functions of cancer/testis-antigens in seminomas, Santa Cruz Biotechnology PRAME antibody (Santa Cruz, H-10) was used in immunohistochemistry on human samples at 1:200 (tbl 1) and in western blot on human samples at 1:300 (tbl 1). Br J Cancer (2016) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunocytochemistry; human; 1:200; tbl 1
  • immunohistochemistry; human; 1:200; loading ...; tbl 1
  • western blot; human; 1:500; tbl 1
In order to research the expression and the functions of cancer/testis-antigens in seminomas, Sigma-Aldrich PRAME antibody (Sigma, HPA045153) was used in immunocytochemistry on human samples at 1:200 (tbl 1), in immunohistochemistry on human samples at 1:200 (tbl 1) and in western blot on human samples at 1:500 (tbl 1). Br J Cancer (2016) ncbi
Articles Reviewed
  1. Lee Y, Park U, Kim E, Hwang J, Jeong J, Um S. Tumor antigen PRAME is up-regulated by MZF1 in cooperation with DNA hypomethylation in melanoma cells. Cancer Lett. 2017;403:144-151 pubmed publisher
  2. Nettersheim D, Arndt I, Sharma R, Riesenberg S, Jostes S, Schneider S, et al. The cancer/testis-antigen PRAME supports the pluripotency network and represses somatic and germ cell differentiation programs in seminomas. Br J Cancer. 2016;115:454-64 pubmed publisher