This is a Validated Antibody Database (VAD) review about human PP1, based on 19 published articles (read how Labome selects the articles), using PP1 antibody in all methods. It is aimed to help Labome visitors find the most suited PP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PP1 synonym: PP-1A; PP1A; PP1alpha; PPP1A; serine/threonine-protein phosphatase PP1-alpha catalytic subunit; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; testicular tissue protein Li 155

Santa Cruz Biotechnology
mouse monoclonal (G-4)
  • western blot; human; loading ...; fig 1c
In order to determine specific residues on four MCM helicase subunits that show hyperphosphorylation upon RIF1 depletion, Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc-271762) was used in western blot on human samples (fig 1c). EMBO Rep (2017) ncbi
mouse monoclonal (E-9)
  • western blot; human; loading ...; tbl 4
Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc-7482) was used in western blot on human samples (tbl 4). Transl Psychiatry (2016) ncbi
mouse monoclonal (E-9)
  • western blot; rat; 1:1000; fig s1
In order to investigate left ventricular cardiomyocytes from post-infaction rats with and without heart failure after beta-adrenoceptor stimulation to revela Ca2+ waves and sarcoplasmic reticulum Ca2+ depletion, Santa Cruz Biotechnology PP1 antibody (Santa Cruz Biotechnology, Sc-7482) was used in western blot on rat samples at 1:1000 (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (E-9)
  • western blot; human; fig s3
Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc-7482) was used in western blot on human samples (fig s3). Genes Dev (2016) ncbi
mouse monoclonal (E-9)
  • immunoprecipitation; human; fig 4B
  • immunocytochemistry; human; fig 4E
  • western blot; human; fig 4B
Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc-7482) was used in immunoprecipitation on human samples (fig 4B), in immunocytochemistry on human samples (fig 4E) and in western blot on human samples (fig 4B). Oncotarget (2016) ncbi
mouse monoclonal (E-9)
  • western blot; mouse; 1:3000; fig 3
Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc7482) was used in western blot on mouse samples at 1:3000 (fig 3). Nat Commun (2015) ncbi
mouse monoclonal (E-9)
  • blocking or activating experiments; human; fig 5
In order to investigate the regulatory mechanism and role of ATG16L1 phosphorylation in autophagy induction using cardiomyocytes, Santa Cruz Biotechnology PP1 antibody (Santa Cruz Biotechnology, SC-7482) was used in blocking or activating experiments on human samples (fig 5). Autophagy (2015) ncbi
mouse monoclonal (E-9)
  • immunoprecipitation; human; 1:2000; fig 1c
In order to show that Sephin1, a selective inhibitor of protein phosphatase 1, protects mice from protein-misfolding disease, Santa Cruz Biotechnology PP1 antibody (Santa, sc-7482) was used in immunoprecipitation on human samples at 1:2000 (fig 1c). Science (2015) ncbi
mouse monoclonal (E-9)
  • western blot; human; 1:1000; fig s1
Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc-7482) was used in western blot on human samples at 1:1000 (fig s1). Oncotarget (2015) ncbi
mouse monoclonal (E-9)
  • western blot; human
In order to study PDE3 in human cardiac myocytes, Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc7482) was used in western blot on human samples . J Biol Chem (2015) ncbi
mouse monoclonal (E-9)
  • immunoprecipitation; rat; 1:300
  • western blot; rat
In order to report the signaling mechanisms by which PKC-zeta activation affects EMAP-II-induced BTB hyperpermeability, Santa Cruz Biotechnology PP1 antibody (Santa Cruz, sc-7482) was used in immunoprecipitation on rat samples at 1:300 and in western blot on rat samples . Exp Cell Res (2015) ncbi
mouse monoclonal (E-9)
  • western blot; green monkey; 1:200
Santa Cruz Biotechnology PP1 antibody (Santa, sc-7482) was used in western blot on green monkey samples at 1:200. J Virol (2014) ncbi
mouse monoclonal (E-9)
  • western blot; human; 1:1000; fig 7
Santa Cruz Biotechnology PP1 antibody (Santa Cruz Biotechnology, sc-7482) was used in western blot on human samples at 1:1000 (fig 7). FEBS Lett (2014) ncbi
Invitrogen
mouse monoclonal (10C6-3)
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
In order to characterize an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications defined by PLEKHA7, Invitrogen PP1 antibody (Invitrogen, 438100) was used in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 1). Cell Cycle (2016) ncbi
mouse monoclonal (10C6-3)
  • western blot; human
In order to elucidate the contribution of lncRNA BCAR4 in breast cancer metastasis, Invitrogen PP1 antibody (Life Technologies, 10C6.3) was used in western blot on human samples . Cell (2014) ncbi
Abcam
rabbit polyclonal
  • western blot; rat; 1:1000; fig 5
In order to learn that imbalance between myocardial phospholamban dephosphorylation and phosphorylation is not caused by obesity, Abcam PP1 antibody (Abcam, AB16446) was used in western blot on rat samples at 1:1000 (fig 5). Arq Bras Cardiol (2014) ncbi
Bethyl
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human; fig 26b
In order to elucidate the contribution of lncRNA BCAR4 in breast cancer metastasis, Bethyl PP1 antibody (Bethyl, A300-904A) was used in chromatin immunoprecipitation on human samples and in western blot on human samples (fig 26b). Cell (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; fig 2
In order to determine the role of Epstein-Barr virus protein kinase integration in mitotic signaling by phosphoproteomic profiling, Cell Signaling Technology PP1 antibody (Cell Signaling Technology, 2582) was used in western blot on human samples (fig 2). PLoS Pathog (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 2
In order to determine the role of Epstein-Barr virus protein kinase integration in mitotic signaling by phosphoproteomic profiling, Cell Signaling Technology PP1 antibody (Cell Signaling Technology, 2581) was used in western blot on human samples (fig 2). PLoS Pathog (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; fig 2
Cell Signaling Technology PP1 antibody (Cell Signaling, 2581S) was used in immunocytochemistry on human samples at 1:1000 (fig 2). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; fruit fly; fig 4b
In order to determine the function of greatwall-phosphorylated endosulfine as a substrate of PP2A-B55 heterotrimers and an inhibitor, Cell Signaling Technology PP1 antibody (Cell Signaling Technology, 2582) was used in western blot on fruit fly samples (fig 4b). elife (2014) ncbi
Articles Reviewed
  1. Hiraga S, Ly T, Garzón J, Horejsi Z, Ohkubo Y, Endo A, et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep. 2017;18:403-419 pubmed publisher
  2. Lopes M, Leal R, Guarnieri R, Schwarzbold M, Hoeller A, Diaz A, et al. A single high dose of dexamethasone affects the phosphorylation state of glutamate AMPA receptors in the human limbic system. Transl Psychiatry. 2016;6:e986 pubmed publisher
  3. Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  4. Kourtidis A, Anastasiadis P. PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Cell Cycle. 2016;15:498-505 pubmed publisher
  5. Sansó M, Levin R, Lipp J, Wang V, Greifenberg A, Quezada E, et al. P-TEFb regulation of transcription termination factor Xrn2 revealed by a chemical genetic screen for Cdk9 substrates. Genes Dev. 2016;30:117-31 pubmed publisher
  6. Li R, Liao G, Nirujogi R, Pinto S, Shaw P, Huang T, et al. Phosphoproteomic Profiling Reveals Epstein-Barr Virus Protein Kinase Integration of DNA Damage Response and Mitotic Signaling. PLoS Pathog. 2015;11:e1005346 pubmed publisher
  7. Qian J, Beullens M, Huang J, De Munter S, Lesage B, Bollen M. Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch. Nat Commun. 2015;6:10215 pubmed publisher
  8. Wang Z, Ma B, Li H, Xiao X, Zhou W, Liu F, et al. Protein 4.1N acts as a potential tumor suppressor linking PP1 to JNK-c-Jun pathway regulation in NSCLC. Oncotarget. 2016;7:509-23 pubmed publisher
  9. Wong P, Feng Y, Wang J, Shi R, Jiang X. Regulation of autophagy by coordinated action of mTORC1 and protein phosphatase 2A. Nat Commun. 2015;6:8048 pubmed publisher
  10. Song H, Pu J, Wang L, Wu L, Xiao J, Liu Q, et al. ATG16L1 phosphorylation is oppositely regulated by CSNK2/casein kinase 2 and PPP1/protein phosphatase 1 which determines the fate of cardiomyocytes during hypoxia/reoxygenation. Autophagy. 2015;11:1308-25 pubmed publisher
  11. Das I, Krzyzosiak A, Schneider K, Wrabetz L, D Antonio M, Barry N, et al. Preventing proteostasis diseases by selective inhibition of a phosphatase regulatory subunit. Science. 2015;348:239-42 pubmed publisher
  12. Li G, Zhou J, Budhraja A, Hu X, Chen Y, Cheng Q, et al. Mitochondrial translocation and interaction of cofilin and Drp1 are required for erucin-induced mitochondrial fission and apoptosis. Oncotarget. 2015;6:1834-49 pubmed
  13. Ahmad F, Shen W, Vandeput F, Szabo Fresnais N, Krall J, Degerman E, et al. Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2. J Biol Chem. 2015;290:6763-76 pubmed publisher
  14. Li Z, Liu Y, Liu X, Xue Y, Wang P, Liu L. Low-dose endothelial monocyte-activating polypeptide-II increases permeability of blood-tumor barrier via a PKC-ζ/PP2A-dependent signaling mechanism. Exp Cell Res. 2015;331:257-66 pubmed publisher
  15. Xing Z, Lin A, Li C, Liang K, Wang S, Liu Y, et al. lncRNA directs cooperative epigenetic regulation downstream of chemokine signals. Cell. 2014;159:1110-1125 pubmed publisher
  16. Freire P, Alves C, Deus A, Leopoldo A, Leopoldo A, Silva D, et al. Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation. Arq Bras Cardiol. 2014;103:41-50 pubmed
  17. Williams B, Filter J, Blake Hodek K, Wadzinski B, Fuda N, Shalloway D, et al. Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers. elife. 2014;3:e01695 pubmed publisher
  18. Jamin A, Wicklund A, Wiebe M. Cell- and virus-mediated regulation of the barrier-to-autointegration factor's phosphorylation state controls its DNA binding, dimerization, subcellular localization, and antipoxviral activity. J Virol. 2014;88:5342-55 pubmed publisher
  19. Amsailale R, Beyaert M, Smal C, Janssens V, Van Den Neste E, Bontemps F. Protein phosphatase 2A regulates deoxycytidine kinase activity via Ser-74 dephosphorylation. FEBS Lett. 2014;588:727-32 pubmed publisher