This is a Validated Antibody Database (VAD) review about human POLG, based on 6 published articles (read how Labome selects the articles), using POLG antibody in all methods. It is aimed to help Labome visitors find the most suited POLG antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
POLG synonym: MDP1; MIRAS; MTDPS4A; MTDPS4B; PEO; POLG1; POLGA; SANDO; SCAE; DNA polymerase subunit gamma-1; PolG-alpha; mitochondrial DNA polymerase catalytic subunit; polymerase (DNA directed), gamma; polymerase (DNA) gamma, catalytic subunit; truncated mitochondrial DNA polymerase gamma catalytic subunit

Abcam
rabbit monoclonal (EPR7296)
  • western blot; human; fig 4a
  • western blot; mouse; loading ...; fig 4c
Abcam POLG antibody (Abcam, ab128899) was used in western blot on human samples (fig 4a) and in western blot on mouse samples (fig 4c). Sci Rep (2017) ncbi
rabbit monoclonal (EPR7296)
  • western blot; human; fig s1d
Abcam POLG antibody (Abcam, ab128899) was used in western blot on human samples (fig s1d). DNA Repair (Amst) (2014) ncbi
rabbit monoclonal (EPR7296)
  • western blot; mouse; fig 2
In order to discuss mitochondrial topoisomerases, Abcam POLG antibody (Abcam, ab128899) was used in western blot on mouse samples (fig 2). PLoS ONE (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-6)
  • western blot; mouse; loading ...; fig 4c
Santa Cruz Biotechnology POLG antibody (SantaCruz, 390634) was used in western blot on mouse samples (fig 4c). PLoS Pathog (2016) ncbi
mouse monoclonal (G-6)
  • western blot; mouse; 1:1000; loading ...; fig 3c
In order to test if heme oxygenase-1 mediates cardiac protection by altering mitochondrial quality control, Santa Cruz Biotechnology POLG antibody (SantaCruz, sc-390634) was used in western blot on mouse samples at 1:1000 (fig 3c). JCI Insight (2016) ncbi
Invitrogen
rabbit polyclonal
  • immunoprecipitation; human; fig 4
  • western blot; human; fig 4
In order to investigate the effects of different p53 isoforms on mitochondrial DNA damage, Invitrogen POLG antibody (Pierce, PA121791) was used in immunoprecipitation on human samples (fig 4) and in western blot on human samples (fig 4). Aging (Albany NY) (2012) ncbi
Articles Reviewed
  1. Zhao X, Sun K, Lan Z, Song W, Cheng L, Chi W, et al. Tenofovir and adefovir down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity. Sci Rep. 2017;7:46344 pubmed publisher
  2. Wan X, Wen J, Koo S, Liang L, Garg N. SIRT1-PGC1α-NFκB Pathway of Oxidative and Inflammatory Stress during Trypanosoma cruzi Infection: Benefits of SIRT1-Targeted Therapy in Improving Heart Function in Chagas Disease. PLoS Pathog. 2016;12:e1005954 pubmed publisher
  3. Hull T, Boddu R, Guo L, Tisher C, Traylor A, Patel B, et al. Heme oxygenase-1 regulates mitochondrial quality control in the heart. JCI Insight. 2016;1:e85817 pubmed
  4. Akbari M, Keijzers G, Maynard S, Scheibye Knudsen M, Desler C, Hickson I, et al. Overexpression of DNA ligase III in mitochondria protects cells against oxidative stress and improves mitochondrial DNA base excision repair. DNA Repair (Amst). 2014;16:44-53 pubmed publisher
  5. Douarre C, Sourbier C, Dalla Rosa I, Brata Das B, Redon C, Zhang H, et al. Mitochondrial topoisomerase I is critical for mitochondrial integrity and cellular energy metabolism. PLoS ONE. 2012;7:e41094 pubmed publisher
  6. Altilia S, Santoro A, Malagoli D, Lanzarini C, Ballesteros Álvarez J, Galazzo G, et al. TP53 codon 72 polymorphism affects accumulation of mtDNA damage in human cells. Aging (Albany NY). 2012;4:28-39 pubmed