This is a Validated Antibody Database (VAD) review about human POLD1, based on 8 published articles (read how Labome selects the articles), using POLD1 antibody in all methods. It is aimed to help Labome visitors find the most suited POLD1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
POLD1 synonym: CDC2; CRCS10; MDPL; POLD; DNA polymerase delta catalytic subunit; CDC2 homolog; DNA polymerase subunit delta p125; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit

Bethyl
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig s7f
  • immunocytochemistry; human; loading ...; fig s6a
  • western blot; human; loading ...; fig s7f
In order to clarify break-induced telomere synthesis and show that it uses a specialized replisome involved in telomere maintenance, Bethyl POLD1 antibody (Bethyl, A304-005A) was used in immunoprecipitation on human samples (fig s7f), in immunocytochemistry on human samples (fig s6a) and in western blot on human samples (fig s7f). Nature (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 5
Bethyl POLD1 antibody (Bethyl Laboratories, A304-007A) was used in western blot on human samples at 1:1000 (fig 5). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 2
Bethyl POLD1 antibody (Bethyl, A304-005A) was used in western blot on human samples (fig 2). Biochim Biophys Acta (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-2)
  • western blot; human; 1:250; loading ...; fig 1d
Santa Cruz Biotechnology POLD1 antibody (Santa Cruz, sc-17 777) was used in western blot on human samples at 1:250 (fig 1d). Nucleic Acids Res (2017) ncbi
mouse monoclonal (A-9)
  • flow cytometry; human; loading ...; fig 2e
Santa Cruz Biotechnology POLD1 antibody (Santa Cruz, sc-17776) was used in flow cytometry on human samples (fig 2e). Sci Rep (2016) ncbi
mouse monoclonal (A-9)
  • western blot; human; 1:500; fig 1
Santa Cruz Biotechnology POLD1 antibody (Santa Cruz, sc-17776) was used in western blot on human samples at 1:500 (fig 1). Nat Commun (2014) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; fig 5b
Proteintech Group POLD1 antibody (Proteintech, 15646-1-AP) was used in western blot on human samples (fig 5b). Hum Mol Genet (2018) ncbi
Abcam
rabbit monoclonal (EPR15118)
  • western blot; human; 1:1000; loading ...; fig 4a
Abcam POLD1 antibody (Abcam, ab186407) was used in western blot on human samples at 1:1000 (fig 4a). Nucleic Acids Res (2017) ncbi
Articles Reviewed
  1. Kim K, Maio N, Singh A, Rouault T. Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Hum Mol Genet. 2018;27:837-852 pubmed publisher
  2. Maskey R, Flatten K, Sieben C, Peterson K, Baker D, Nam H, et al. Spartan deficiency causes accumulation of Topoisomerase 1 cleavage complexes and tumorigenesis. Nucleic Acids Res. 2017;45:4564-4576 pubmed publisher
  3. Tumini E, Barroso S, Calero C, Aguilera A. Roles of human POLD1 and POLD3 in genome stability. Sci Rep. 2016;6:38873 pubmed publisher
  4. Dilley R, Verma P, Cho N, Winters H, Wondisford A, Greenberg R. Break-induced telomere synthesis underlies alternative telomere maintenance. Nature. 2016;539:54-58 pubmed publisher
  5. Wu R, Wang Z, Zhang H, Gan H, Zhang Z. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res. 2017;45:169-180 pubmed publisher
  6. Passerini V, Ozeri Galai E, de Pagter M, Donnelly N, Schmalbrock S, Kloosterman W, et al. The presence of extra chromosomes leads to genomic instability. Nat Commun. 2016;7:10754 pubmed publisher
  7. Benatti P, Belluti S, Miotto B, Neusiedler J, Dolfini D, Drac M, et al. Direct non transcriptional role of NF-Y in DNA replication. Biochim Biophys Acta. 2016;1863:673-85 pubmed publisher
  8. Fernandez Vidal A, Guitton Sert L, Cadoret J, Drac M, Schwob E, Baldacci G, et al. A role for DNA polymerase ? in the timing of DNA replication. Nat Commun. 2014;5:4285 pubmed publisher