This is a Validated Antibody Database (VAD) review about human POLA1, based on 8 published articles (read how Labome selects the articles), using POLA1 antibody in all methods. It is aimed to help Labome visitors find the most suited POLA1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
POLA1 synonym: NSX; POLA; p180; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD)

Abcam
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1d
Abcam POLA1 antibody (Abcam, ab31777) was used in western blot on human samples at 1:1000 (fig 1d). Science (2017) ncbi
rabbit polyclonal
  • western blot; common platanna; 1:1000; fig 8
Abcam POLA1 antibody (Abcam, ab31777) was used in western blot on common platanna samples at 1:1000 (fig 8). Nat Commun (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4
Abcam POLA1 antibody (Abcam, ab31777) was used in chromatin immunoprecipitation on human samples (fig 4). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • western blot; rat
Abcam POLA1 antibody (Abcam, ab311777) was used in western blot on rat samples . Nat Commun (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-12)
  • western blot; mouse; fig s1
Santa Cruz Biotechnology POLA1 antibody (Santa Cruz, sc- 365039) was used in western blot on mouse samples (fig s1). Cell Rep (2016) ncbi
mouse monoclonal (D-7)
  • western blot; human; fig 4
Santa Cruz Biotechnology POLA1 antibody (Santa Cruz, sc-137021) was used in western blot on human samples (fig 4). Oncol Rep (2015) ncbi
Bethyl
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig s6a
In order to clarify break-induced telomere synthesis and show that it uses a specialized replisome involved in telomere maintenance, Bethyl POLA1 antibody (Bethyl, A302-851A) was used in immunocytochemistry on human samples (fig s6a). Nature (2016) ncbi
BD Biosciences
mouse monoclonal (22/DNA Polymerase)
  • dot blot; human; 1:500; loading ...; fig 2a
BD Biosciences POLA1 antibody (BD Biosciences, 610972) was used in dot blot on human samples at 1:500 (fig 2a). PLoS ONE (2016) ncbi
Articles Reviewed
  1. Somyajit K, Gupta R, Sedlackova H, Neelsen K, Ochs F, Rask M, et al. Redox-sensitive alteration of replisome architecture safeguards genome integrity. Science. 2017;358:797-802 pubmed publisher
  2. Li C, Miao Y, Lingeman R, Hickey R, Malkas L. Partial Purification of a Megadalton DNA Replication Complex by Free Flow Electrophoresis. PLoS ONE. 2016;11:e0169259 pubmed publisher
  3. Dilley R, Verma P, Cho N, Winters H, Wondisford A, Greenberg R. Break-induced telomere synthesis underlies alternative telomere maintenance. Nature. 2016;539:54-58 pubmed publisher
  4. Simon A, Sannino V, Costanzo V, Pellegrini L. Structure of human Cdc45 and implications for CMG helicase function. Nat Commun. 2016;7:11638 pubmed publisher
  5. Ho T, Guilbaud G, Blow J, Sale J, Watson C. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Rep. 2016;15:724-734 pubmed publisher
  6. Yun J, Song S, Kang J, Park J, Kim H, Han S, et al. Reduced cohesin destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability. Nucleic Acids Res. 2016;44:558-72 pubmed publisher
  7. Wang C, Zhang W, Fu M, Yang A, Huang H, Xie J. Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression. Oncol Rep. 2015;33:383-90 pubmed publisher
  8. Fernandez Vidal A, Guitton Sert L, Cadoret J, Drac M, Schwob E, Baldacci G, et al. A role for DNA polymerase ? in the timing of DNA replication. Nat Commun. 2014;5:4285 pubmed publisher