This is a Validated Antibody Database (VAD) review about human PMEL, based on 24 published articles (read how Labome selects the articles), using PMEL antibody in all methods. It is aimed to help Labome visitors find the most suited PMEL antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PMEL synonym: D12S53E; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV; gp100

Abcam
domestic rabbit monoclonal (EP4863(2))
  • immunocytochemistry; human; 1:100; fig 3c
Abcam PMEL antibody (Abcam, ab137078) was used in immunocytochemistry on human samples at 1:100 (fig 3c). Front Cell Dev Biol (2021) ncbi
domestic rabbit monoclonal (EP4863(2))
  • immunocytochemistry; human; loading ...; fig 3g
Abcam PMEL antibody (Abcam, ab137078) was used in immunocytochemistry on human samples (fig 3g). Stem Cell Res Ther (2021) ncbi
domestic rabbit monoclonal (EP4863(2))
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1e
Abcam PMEL antibody (Abcam, ab137078) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 1e). elife (2020) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry; human; 1:100; loading ...; fig 4d
Abcam PMEL antibody (Abcam, ab787) was used in immunohistochemistry on human samples at 1:100 (fig 4d). elife (2019) ncbi
domestic rabbit monoclonal (EP4863(2))
  • immunohistochemistry - frozen section; mouse; loading ...; fig s1b
Abcam PMEL antibody (abcam, ab137078) was used in immunohistochemistry - frozen section on mouse samples (fig s1b). Cell Rep (2018) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry; human; loading ...; fig 1
In order to test if vascular normalization with an antiangiogenic monoclonal antibody enhances detection of melanoma, Abcam PMEL antibody (Abcam, 787) was used in immunohistochemistry on human samples (fig 1). Laryngoscope (2016) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry; human; 1:500; fig 3
Abcam PMEL antibody (Abcam, ab787) was used in immunohistochemistry on human samples at 1:500 (fig 3). Oncol Lett (2016) ncbi
domestic rabbit monoclonal (EP4863(2))
  • western blot; human; fig 3e
Abcam PMEL antibody (Abcam, ab137078) was used in western blot on human samples (fig 3e). Nat Genet (2016) ncbi
mouse monoclonal (HMB45)
  • immunocytochemistry; human; loading ...
In order to study Isolation of individual cellular components from lung tissues of patients with lymphangioleiomyomatosis, Abcam PMEL antibody (Abcam, ab787) was used in immunocytochemistry on human samples . Am J Physiol Lung Cell Mol Physiol (2016) ncbi
domestic goat polyclonal
  • western blot; mouse; 1:250; fig 1
In order to report that BACE2 and BACE1 inhibition in mice affects melanosome maturation and induces hair depigmentation, Abcam PMEL antibody (abcam, Ab52058) was used in western blot on mouse samples at 1:250 (fig 1). Sci Rep (2016) ncbi
domestic rabbit monoclonal (EP4863(2))
  • western blot; mouse; fig 4
In order to study engineered anti-melanoma vaccines that modulate cytokine priming and silence PD-L1 simultaneously by use of ex vivo myeloid-derived suppressor cells as a readout of therapeutic efficacy, Abcam PMEL antibody (Abcam, ab137078) was used in western blot on mouse samples (fig 4). Oncoimmunology (2014) ncbi
Invitrogen
mouse monoclonal (HMB45)
  • immunocytochemistry; human; 1:40; loading ...; fig 2a
In order to identify conditions in which low-passage ARPE-19 cells express genes specific to native human RPE cells, Invitrogen PMEL antibody (Thermo Fisher Scientific, MA5-13232) was used in immunocytochemistry on human samples at 1:40 (fig 2a). Mol Vis (2017) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry - frozen section; human; 1:150; loading ...; fig 1d
In order to investigate the role of natural killer cells to lymphangioleiomyomatosis pathogenesis, Invitrogen PMEL antibody (Thermo Fisher Scientific, HMB45) was used in immunohistochemistry - frozen section on human samples at 1:150 (fig 1d). JCI Insight (2016) ncbi
mouse monoclonal (HMB45)
  • immunocytochemistry; mouse; fig 3o
In order to propose that VAMP7 mediates fusion of BLOC-1-dependent transport carriers with melanosomes, Invitrogen PMEL antibody (Lab Vision, HMB-45) was used in immunocytochemistry on mouse samples (fig 3o). J Cell Biol (2016) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry - paraffin section; human; fig 1c
In order to develop a patient-derived xenograft platform and use it to identify genes that contribute the cancer resistance of melanoma patients treated with BRAF inhibitors, Invitrogen PMEL antibody (Thermo Scientific, MS-264-S0) was used in immunohistochemistry - paraffin section on human samples (fig 1c). Cell Rep (2016) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 1
In order to study an increasing melanoma cell growth in the bone marrow by a high fat diet that induces osteopontin and interleukin 6, Invitrogen PMEL antibody (Pierce, MA1-34759) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 1). Oncotarget (2016) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry; human; 1:80; fig 10
In order to present a case of highly aggressive ocular melanoma, Invitrogen PMEL antibody (Thermo Fisher Scientific, HMB-45) was used in immunohistochemistry on human samples at 1:80 (fig 10). Rom J Morphol Embryol (2015) ncbi
mouse monoclonal (HMB45)
  • flow cytometry; mouse; fig s1
In order to investigate the role of BLOC-2 in endosomal tubular transport in melanosomes, Invitrogen PMEL antibody (Lab Vision, HMB-45 (clone)) was used in flow cytometry on mouse samples (fig s1). J Cell Biol (2015) ncbi
mouse monoclonal (HMB45)
  • western blot; mouse
In order to determine which HDAC to target to specifically inhibit metastatic melanoma, Invitrogen PMEL antibody (Thermo Scientific, MA1-34759) was used in western blot on mouse samples . Mol Oncol (2015) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry - paraffin section; human; 1:80
Invitrogen PMEL antibody (Thermo Fisher Scientific, HMB-45) was used in immunohistochemistry - paraffin section on human samples at 1:80. Rom J Morphol Embryol (2014) ncbi
mouse monoclonal (HMB45)
  • western blot; human
In order to identify antigens recognized by autoreactive CD4 T lymphocytes isolated from a Vogt-Koyanagi-Harada patient who did not express HLA-DRB1*04:05, Invitrogen PMEL antibody (Thermo Scientific, MA5-13232) was used in western blot on human samples . Mol Vis (2014) ncbi
mouse monoclonal (HMB45)
  • immunohistochemistry - paraffin section; human; 1:40
In order to present two cases of epithelioid PEComas, Invitrogen PMEL antibody (Neomarkers, HMB-45) was used in immunohistochemistry - paraffin section on human samples at 1:40. Case Rep Med (2012) ncbi
mouse monoclonal (HMB45)
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 1
In order to study Pmel17 processing in melanocytes, Invitrogen PMEL antibody (NeoMarkers, HMB45) was used in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 1). J Biol Chem (2011) ncbi
mouse monoclonal (HMB45)
  • flow cytometry; human
  • immunocytochemistry; human
  • western blot; human
  • immunocytochemistry; mouse
In order to investigate the role of BLOC-1 in melanosome maturation, Invitrogen PMEL antibody (Lab Vision, HMB-45) was used in flow cytometry on human samples , in immunocytochemistry on human samples , in western blot on human samples and in immunocytochemistry on mouse samples . Mol Biol Cell (2007) ncbi
Articles Reviewed
  1. Li J, Qiu C, Wei Y, Yuan W, Liu J, Cui W, et al. Human Amniotic Epithelial Stem Cell-Derived Retinal Pigment Epithelium Cells Repair Retinal Degeneration. Front Cell Dev Biol. 2021;9:737242 pubmed publisher
  2. Surendran H, Nandakumar S, Reddy K V, Stoddard J, Mohan K V, Upadhyay P, et al. Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration. Stem Cell Res Ther. 2021;12:70 pubmed publisher
  3. Ruiz Vega R, Chen C, Razzak E, Vasudeva P, Krasieva T, Shiu J, et al. Dynamics of nevus development implicate cell cooperation in the growth arrest of transformed melanocytes. elife. 2020;9: pubmed publisher
  4. Achberger K, Probst C, Haderspeck J, Bolz S, Rogal J, Chuchuy J, et al. Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform. elife. 2019;8: pubmed publisher
  5. Commerford C, Dieterich L, He Y, Hell T, Montoya Zegarra J, Noerrelykke S, et al. Mechanisms of Tumor-Induced Lymphovascular Niche Formation in Draining Lymph Nodes. Cell Rep. 2018;25:3554-3563.e4 pubmed publisher
  6. Samuel W, Jaworski C, Postnikova O, Kutty R, Duncan T, Tan L, et al. Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells. Mol Vis. 2017;23:60-89 pubmed
  7. Osterburg A, Nelson R, Yaniv B, Foot R, Donica W, Nashu M, et al. NK cell activating receptor ligand expression in lymphangioleiomyomatosis is associated with lung function decline. JCI Insight. 2016;1:e87270 pubmed publisher
  8. Sweeny L, Prince A, Patel N, Moore L, Rosenthal E, Hughley B, et al. Antiangiogenic antibody improves melanoma detection by fluorescently labeled therapeutic antibodies. Laryngoscope. 2016;126:E387-E395 pubmed publisher
  9. Dennis M, Delevoye C, Acosta Ruiz A, Hurbain I, Romao M, Hesketh G, et al. BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers. J Cell Biol. 2016;214:293-308 pubmed publisher
  10. Kemper K, Krijgsman O, Kong X, Cornelissen Steijger P, Shahrabi A, Weeber F, et al. BRAF(V600E) Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts. Cell Rep. 2016;16:263-277 pubmed publisher
  11. Liu W, Meng Z, Liu H, Li W, Wu Q, Zhang X, et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett. 2016;11:3669-3675 pubmed
  12. Moore A, Ceraudo E, Sher J, Guan Y, Shoushtari A, Chang M, et al. Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma. Nat Genet. 2016;48:675-80 pubmed publisher
  13. Chen G, Luo Y, Eriksson D, Meng X, Qian C, Bauerle T, et al. High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6. Oncotarget. 2016;7:26653-69 pubmed publisher
  14. Ando K, Fujino N, Mitani K, Ota C, Okada Y, Kondo T, et al. Isolation of individual cellular components from lung tissues of patients with lymphangioleiomyomatosis. Am J Physiol Lung Cell Mol Physiol. 2016;310:L899-908 pubmed publisher
  15. Shimshek D, Jacobson L, Kolly C, Zamurovic N, Balavenkatraman K, Morawiec L, et al. Pharmacological BACE1 and BACE2 inhibition induces hair depigmentation by inhibiting PMEL17 processing in mice. Sci Rep. 2016;6:21917 pubmed publisher
  16. Costache M, Dumitru A, Pătraşcu O, Popa Cherecheanu D, Bădilă P, Miu J, et al. A challenging case of ocular melanoma. Rom J Morphol Embryol. 2015;56:817-22 pubmed
  17. Dennis M, Mantegazza A, Snir O, Tenza D, Acosta Ruiz A, Delevoye C, et al. BLOC-2 targets recycling endosomal tubules to melanosomes for cargo delivery. J Cell Biol. 2015;209:563-77 pubmed publisher
  18. Woan K, Lienlaf M, Perez Villaroel P, Lee C, Cheng F, Knox T, et al. Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation. Mol Oncol. 2015;9:1447-1457 pubmed publisher
  19. Liechtenstein T, Perez Janices N, Blanco Luquin I, Goyvaerts C, Schwarze J, Dufait I, et al. Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using ex vivo myeloid-derived suppressor cells as a readout of therapeutic efficacy. Oncoimmunology. 2014;3:e945378 pubmed
  20. Costache M, Pătraşcu O, Dumitru A, Costache D, Voinea L, Simionescu O, et al. Histopathological findings concerning ocular melanomas. Rom J Morphol Embryol. 2014;55:649-53 pubmed
  21. Abad S, Wieërs G, Colau D, Wildmann C, Delair E, Dhote R, et al. Absence of recognition of common melanocytic antigens by T cells isolated from the cerebrospinal fluid of a Vogt-Koyanagi-Harada patient. Mol Vis. 2014;20:956-69 pubmed
  22. Niu H, Wang F, Zhang P, Bing Z. Cardiac Epithelioid PEComa: Report of Two Cases and Review of the Literature. Case Rep Med. 2012;2012:521678 pubmed publisher
  23. Leonhardt R, Vigneron N, Rahner C, Cresswell P. Proprotein convertases process Pmel17 during secretion. J Biol Chem. 2011;286:9321-37 pubmed publisher
  24. Setty S, Tenza D, Truschel S, Chou E, Sviderskaya E, Theos A, et al. BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles. Mol Biol Cell. 2007;18:768-80 pubmed