This is a Validated Antibody Database (VAD) review about human PLK4, based on 8 published articles (read how Labome selects the articles), using PLK4 antibody in all methods. It is aimed to help Labome visitors find the most suited PLK4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PLK4 synonym: MCCRP2; SAK; STK18; serine/threonine-protein kinase PLK4; Snk akin kinase; serine/threonine-protein kinase 18; serine/threonine-protein kinase Sak

Knockout validation
Abcam
goat polyclonal
  • immunohistochemistry knockout validation; human; 1:150; fig 2
  • immunohistochemistry knockout validation; mouse; 1:150; fig 3
Abcam PLK4 antibody (Abcam, Ab2642) was used in immunohistochemistry knockout validation on human samples at 1:150 (fig 2) and in immunohistochemistry knockout validation on mouse samples at 1:150 (fig 3). PLoS ONE (2016) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry; human; 1:100; fig 2
Abcam PLK4 antibody (abcam, ab137398) was used in immunohistochemistry on human samples at 1:100 (fig 2). J Cancer (2016) ncbi
goat polyclonal
  • immunohistochemistry knockout validation; human; 1:150; fig 2
  • immunohistochemistry knockout validation; mouse; 1:150; fig 3
Abcam PLK4 antibody (Abcam, Ab2642) was used in immunohistochemistry knockout validation on human samples at 1:150 (fig 2) and in immunohistochemistry knockout validation on mouse samples at 1:150 (fig 3). PLoS ONE (2016) ncbi
goat polyclonal
  • immunocytochemistry; mouse; 1:100; fig 5
In order to show that Plk4 promotes cell migration, Abcam PLK4 antibody (Abcam, ab2642) was used in immunocytochemistry on mouse samples at 1:100 (fig 5). Oncogene (2015) ncbi
Abnova
mouse polyclonal
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000
In order to assess PCM1 phosphorylation as a non-canonical function of Plk4 in centriolar satellite integrity and ciliogenesis, Abnova PLK4 antibody (Abnova, H00010733-B01) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:1000. EMBO Rep (2016) ncbi
mouse polyclonal
  • immunocytochemistry; human; 1:200
In order to demonstrate that hMsd1/SSX2IP-mediated microtubule anchoring is required for proper centriole assembly and duplication, Abnova PLK4 antibody (Abnova, H00010733-B01) was used in immunocytochemistry on human samples at 1:200. Mol Biol Cell (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 3
Cell Signaling Technology PLK4 antibody (Cell Signaling, 3258) was used in western blot on human samples (fig 3). Proc Natl Acad Sci U S A (2019) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology PLK4 antibody (Cell Signaling, 3258S) was used in western blot on human samples . Cell Cycle (2013) ncbi
EMD Millipore
mouse monoclonal (6H5)
  • immunocytochemistry; human; 1:500
EMD Millipore PLK4 antibody (Millipore, MABC544) was used in immunocytochemistry on human samples at 1:500. Nat Commun (2014) ncbi
Articles Reviewed
  1. Press M, Xie B, Davenport S, Zhou Y, Guzman R, Nolan G, et al. Role for polo-like kinase 4 in mediation of cytokinesis. Proc Natl Acad Sci U S A. 2019;116:11309-11318 pubmed publisher
  2. Li Z, Dai K, Wang C, Song Y, Gu F, Liu F, et al. Expression of Polo-Like Kinase 4(PLK4) in Breast Cancer and Its Response to Taxane-Based Neoadjuvant Chemotherapy. J Cancer. 2016;7:1125-32 pubmed publisher
  3. Czub B, Shah A, Alfano G, Kruczek P, Chakarova C, Bhattacharya S. TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene. PLoS ONE. 2016;11:e0148678 pubmed publisher
  4. Hori A, Barnouin K, Snijders A, Toda T. A non-canonical function of Plk4 in centriolar satellite integrity and ciliogenesis through PCM1 phosphorylation. EMBO Rep. 2016;17:326-37 pubmed publisher
  5. Hori A, Peddie C, Collinson L, Toda T. Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly. Mol Biol Cell. 2015;26:2005-19 pubmed publisher
  6. Ohta M, Ashikawa T, Nozaki Y, Kozuka Hata H, Goto H, Inagaki M, et al. Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole. Nat Commun. 2014;5:5267 pubmed publisher
  7. Rosario C, Kazazian K, Zih F, Brashavitskaya O, Haffani Y, Xu R, et al. A novel role for Plk4 in regulating cell spreading and motility. Oncogene. 2015;34:3441-51 pubmed publisher
  8. Ledoux A, Sellier H, Gillies K, Iannetti A, James J, Perkins N. NF?B regulates expression of Polo-like kinase 4. Cell Cycle. 2013;12:3052-62 pubmed publisher