This is a Validated Antibody Database (VAD) review about human PKP3, based on 8 published articles (read how Labome selects the articles), using PKP3 antibody in all methods. It is aimed to help Labome visitors find the most suited PKP3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Abcam
domestic rabbit monoclonal (EPR5560)
  • immunohistochemistry; mouse; fig 8g
Abcam PKP3 antibody (Abcam, ab247865) was used in immunohistochemistry on mouse samples (fig 8g). Mol Cancer (2021) ncbi
domestic rabbit monoclonal (EPR5560)
  • western blot; human; 1:10,000; loading ...; fig 3s2a
Abcam PKP3 antibody (Abcam, ab109441) was used in western blot on human samples at 1:10,000 (fig 3s2a). elife (2019) ncbi
Invitrogen
mouse monoclonal (23E3/4)
  • western blot; human; 1:2000
In order to investigate the role of MMP7 in cell invasion and tumor formation, Invitrogen PKP3 antibody (Invitrogen, 35-7600) was used in western blot on human samples at 1:2000. PLoS ONE (2015) ncbi
mouse monoclonal (23E3/4)
  • immunocytochemistry; mouse; 1:250; fig 1
In order to explore the role of desmosomal cadherins in carcinogenesis, Invitrogen PKP3 antibody (Zymed, 23E3/4) was used in immunocytochemistry on mouse samples at 1:250 (fig 1). PLoS ONE (2012) ncbi
mouse monoclonal (23E3/4)
  • western blot; human; 1:1000
In order to investigate the effects of K8 filament stabilization, Invitrogen PKP3 antibody (Zymed, 23E3-4) was used in western blot on human samples at 1:1000. PLoS ONE (2012) ncbi
mouse monoclonal (23E3/4)
  • immunocytochemistry; human; 1:25; fig 5
  • western blot; human; 1:1000; fig 2
In order to test if plakophilin3 is required for desmosome formation, Invitrogen PKP3 antibody (Zymed, clone 23E3-4) was used in immunocytochemistry on human samples at 1:25 (fig 5) and in western blot on human samples at 1:1000 (fig 2). Cell Mol Life Sci (2011) ncbi
mouse monoclonal (23E3/4)
  • chromatin immunoprecipitation; human; fig 3
  • immunocytochemistry; human; fig 2
  • immunohistochemistry; human; fig 5
  • western blot; human; fig 1
In order to show that PKP3 is transcriptionally repressed by ZEB1 in metastatic cancer cells, Invitrogen PKP3 antibody (Zymed Laboratories, 23E3/4) was used in chromatin immunoprecipitation on human samples (fig 3), in immunocytochemistry on human samples (fig 2), in immunohistochemistry on human samples (fig 5) and in western blot on human samples (fig 1). FEBS Lett (2007) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-10)
  • western blot; human; 1:200; loading ...; fig 1b
Santa Cruz Biotechnology PKP3 antibody (Santa Cruz, sc-166655) was used in western blot on human samples at 1:200 (fig 1b). Sci Rep (2021) ncbi
Articles Reviewed
  1. Liu Z, Wang T, She Y, Wu K, Gu S, Li L, et al. N6-methyladenosine-modified circIGF2BP3 inhibits CD8+ T-cell responses to facilitate tumor immune evasion by promoting the deubiquitination of PD-L1 in non-small cell lung cancer. Mol Cancer. 2021;20:105 pubmed publisher
  2. Shelton W, Thomas S, Alexander H, Thomes C, Conway D, Dubash A. Desmoglein-2 harnesses a PDZ-GEF2/Rap1 signaling axis to control cell spreading and focal adhesions independent of cell-cell adhesion. Sci Rep. 2021;11:13295 pubmed publisher
  3. Fearnley G, Young K, Edgar J, Antrobus R, Hay I, Liang W, et al. The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. elife. 2019;8: pubmed publisher
  4. Basu S, Thorat R, Dalal S. MMP7 is required to mediate cell invasion and tumor formation upon Plakophilin3 loss. PLoS ONE. 2015;10:e0123979 pubmed publisher
  5. Baron S, Hoang A, Vogel H, Attardi L. Unimpaired skin carcinogenesis in Desmoglein 3 knockout mice. PLoS ONE. 2012;7:e50024 pubmed publisher
  6. Khapare N, Kundu S, Sehgal L, Sawant M, Priya R, Gosavi P, et al. Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis. PLoS ONE. 2012;7:e38561 pubmed publisher
  7. Gosavi P, Kundu S, Khapare N, Sehgal L, Karkhanis M, Dalal S. E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly. Cell Mol Life Sci. 2011;68:1439-54 pubmed publisher
  8. Aigner K, Descovich L, Mikula M, Sultan A, Dampier B, Bonne S, et al. The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression. FEBS Lett. 2007;581:1617-24 pubmed