This is a Validated Antibody Database (VAD) review about human PKN2, based on 6 published articles (read how Labome selects the articles), using PKN2 antibody in all methods. It is aimed to help Labome visitors find the most suited PKN2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PKN2 synonym: PAK2; PRK2; PRKCL2; PRO2042; Pak-2; STK7

Knockout validation
R&D Systems
mouse monoclonal (509105)
  • immunohistochemistry knockout validation; mouse; fig s2
R&D Systems PKN2 antibody (R&D Systems, 509105) was used in immunohistochemistry knockout validation on mouse samples (fig s2). Cell Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2a
Cell Signaling Technology PKN2 antibody (Cell Signaling, 2612) was used in western blot knockout validation on human samples at 1:1000 (fig 2a). elife (2019) ncbi
Abcam
domestic rabbit monoclonal (E352)
  • western blot; human; fig 6
Abcam PKN2 antibody (Abcam, ab32395) was used in western blot on human samples (fig 6). elife (2016) ncbi
domestic rabbit monoclonal (EPR5490)
  • immunoprecipitation; human; 1:200; fig 3
  • western blot; human; 1:1000; fig 3
In order to learn regulation of actin network architecture and ciliogenesis due to STAR syndrome-associated CDK10/Cyclin M, Abcam PKN2 antibody (Abcam, ab138514) was used in immunoprecipitation on human samples at 1:200 (fig 3) and in western blot on human samples at 1:1000 (fig 3). Cell Cycle (2016) ncbi
R&D Systems
mouse monoclonal (509105)
  • immunohistochemistry knockout validation; mouse; fig s2
R&D Systems PKN2 antibody (R&D Systems, 509105) was used in immunohistochemistry knockout validation on mouse samples (fig s2). Cell Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2a
Cell Signaling Technology PKN2 antibody (Cell Signaling, 2612) was used in western blot knockout validation on human samples at 1:1000 (fig 2a). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology PKN2 antibody (Cell Signaling, 2612) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3b
Cell Signaling Technology PKN2 antibody (Cell Signaling, 2612) was used in western blot on mouse samples (fig 3b). Nat Immunol (2016) ncbi
Articles Reviewed
  1. Zeng H, Castillo Cabrera J, Manser M, Lu B, Yang Z, Strande V, et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. elife. 2019;8: pubmed publisher
  2. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  3. Bercovich Kinori A, Tai J, Gelbart I, Shitrit A, Ben Moshe S, Drori Y, et al. A systematic view on influenza induced host shutoff. elife. 2016;5: pubmed publisher
  4. Park Y, Wood G, Kastner D, Chae J. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol. 2016;17:914-21 pubmed publisher
  5. Guen V, Gamble C, Perez D, Bourassa S, Zappel H, Gartner J, et al. STAR syndrome-associated CDK10/Cyclin M regulates actin network architecture and ciliogenesis. Cell Cycle. 2016;15:678-88 pubmed publisher
  6. Quétier I, Marshall J, Spencer Dene B, Lachmann S, Casamassima A, Franco C, et al. Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion. Cell Rep. 2016;14:440-448 pubmed publisher