This is a Validated Antibody Database (VAD) review about human PINCH, based on 6 published articles (read how Labome selects the articles), using PINCH antibody in all methods. It is aimed to help Labome visitors find the most suited PINCH antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PINCH synonym: PINCH; PINCH-1; PINCH1; LIM and senescent cell antigen-like-containing domain protein 1; LIM and senescent cell antigen-like domains 1; LIM-type zinc finger domains 1; particularly interesting new Cys-His protein 1; renal carcinoma antigen NY-REN-48; senescent cell antigen

Knockout validation
BD Biosciences
mouse monoclonal (49/PINCH)
  • western blot; rat; fig 1
  • western blot knockout validation; mouse; fig 2a
In order to generate and characterize PINCH 1 and PINCH 2 double knockout mice, BD Biosciences PINCH antibody (BD, 612710) was used in western blot on rat samples (fig 1) and in western blot knockout validation on mouse samples (fig 2a). PLoS ONE (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (49)
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 2
  • western blot; human; loading ...; fig 3
Santa Cruz Biotechnology PINCH antibody (Santa Cruz Biotechnology, sc-136299) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 2) and in western blot on human samples (fig 3). Exp Ther Med (2016) ncbi
BD Biosciences
mouse monoclonal (49/PINCH)
  • immunocytochemistry; mouse; fig 5
  • western blot; mouse; fig 2
In order to learn about the control of differentiation in mammary epithelial cells by the integrin-mediated ILK-parvin-alpha-Pix signaling axis, BD Biosciences PINCH antibody (BD Transduction, 612710) was used in immunocytochemistry on mouse samples (fig 5) and in western blot on mouse samples (fig 2). J Cell Physiol (2016) ncbi
mouse monoclonal (49/PINCH)
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2c
BD Biosciences PINCH antibody (BD, 612711) was used in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 2c). Oncotarget (2015) ncbi
mouse monoclonal (49/PINCH)
  • immunoprecipitation; hamsters; 1 ug/ml
  • western blot; hamsters; 0.25 ug/ml
BD Biosciences PINCH antibody (BD Biosciences, 49/PINCH) was used in immunoprecipitation on hamsters samples at 1 ug/ml and in western blot on hamsters samples at 0.25 ug/ml. PLoS ONE (2013) ncbi
mouse monoclonal (49/PINCH)
  • western blot; rat; fig 1
  • western blot knockout validation; mouse; fig 2a
In order to generate and characterize PINCH 1 and PINCH 2 double knockout mice, BD Biosciences PINCH antibody (BD, 612710) was used in western blot on rat samples (fig 1) and in western blot knockout validation on mouse samples (fig 2a). PLoS ONE (2013) ncbi
Sigma-Aldrich
mouse monoclonal (PINCH-C58)
  • western blot; human; 1:750; fig 1
Sigma-Aldrich PINCH antibody (Sigma, P8996) was used in western blot on human samples at 1:750 (fig 1). Sci Rep (2015) ncbi
Articles Reviewed
  1. Peng Y, Shi X, Li Z, He X, Sun Y. Particularly interesting Cys-His-rich protein is highly expressed in human intracranial aneurysms and resists aneurysmal rupture. Exp Ther Med. 2016;12:3905-3912 pubmed publisher
  2. Rooney N, Wang P, Brennan K, Gilmore A, Streuli C. The Integrin-Mediated ILK-Parvin-?Pix Signaling Axis Controls Differentiation in Mammary Epithelial Cells. J Cell Physiol. 2016;231:2408-17 pubmed publisher
  3. Hausmann C, Temme A, Cordes N, Eke I. ILKAP, ILK and PINCH1 control cell survival of p53-wildtype glioblastoma cells after irradiation. Oncotarget. 2015;6:34592-605 pubmed publisher
  4. Morris E, Assi K, Salh B, Dedhar S. Integrin-linked kinase links dynactin-1/dynactin-2 with cortical integrin receptors to orient the mitotic spindle relative to the substratum. Sci Rep. 2015;5:8389 pubmed publisher
  5. Honda S, Shirotani Ikejima H, Tadokoro S, Tomiyama Y, Miyata T. The integrin-linked kinase-PINCH-parvin complex supports integrin ?IIb?3 activation. PLoS ONE. 2013;8:e85498 pubmed publisher
  6. Donthamsetty S, Bhave V, Mars W, Bowen W, Orr A, Haynes M, et al. Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and tumorigenesis. PLoS ONE. 2013;8:e74625 pubmed publisher