This is a Validated Antibody Database (VAD) review about human PIK3R4, based on 5 published articles (read how Labome selects the articles), using PIK3R4 antibody in all methods. It is aimed to help Labome visitors find the most suited PIK3R4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PIK3R4 synonym: VPS15; p150; phosphoinositide 3-kinase regulatory subunit 4; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide-3-kinase, regulatory subunit 4, p150

Novus Biologicals
rabbit polyclonal
  • western blot; mouse; 1:2000; loading ...; fig 2a
Novus Biologicals PIK3R4 antibody (Novus Biologicals, NBP1-30463) was used in western blot on mouse samples at 1:2000 (fig 2a). Nat Neurosci (2018) ncbi
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 4b
  • immunocytochemistry; human; loading ...; fig 5b
  • western blot; human; loading ...; fig 4b
In order to investigate the contribution of PIK3R4 mutations to ciliopathies, Novus Biologicals PIK3R4 antibody (Novus Biologicals, NBP1-30463) was used in immunoprecipitation on human samples (fig 4b), in immunocytochemistry on human samples (fig 5b) and in western blot on human samples (fig 4b). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; fig s7
In order to use of chaperone-mediated autophagy to facilitate lipolysis by degradation of lipid droplet-associated proteins, Novus Biologicals PIK3R4 antibody (Novus Biologicals, NBP130463) was used in immunocytochemistry on mouse samples at 1:100 (fig s7). Nat Cell Biol (2015) ncbi
Bethyl
rabbit polyclonal
  • western blot; mouse
Bethyl PIK3R4 antibody (Bethyl Laboratories, A302-571A) was used in western blot on mouse samples . Skelet Muscle (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:4000
In order to identify and characterize the Nrbf2-Beclin 1 interaction, Bethyl PIK3R4 antibody (Bethyl, A302-571A) was used in western blot on human samples at 1:4000. J Biol Chem (2014) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig s2g
Proteintech Group PIK3R4 antibody (Proteintech, 17894-1- AP) was used in western blot on mouse samples at 1:500 (fig s2g). Nat Neurosci (2018) ncbi
Articles Reviewed
  1. Gstrein T, Edwards A, Přistoupilová A, Leca I, Breuss M, Pilat Carotta S, et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat Neurosci. 2018;21:207-217 pubmed publisher
  2. Stoetzel C, Bär S, De Craene J, Scheidecker S, Etard C, Chicher J, et al. A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi. Nat Commun. 2016;7:13586 pubmed publisher
  3. Foltz S, Luan J, Call J, Patel A, Peissig K, Fortunato M, et al. Four-week rapamycin treatment improves muscular dystrophy in a fukutin-deficient mouse model of dystroglycanopathy. Skelet Muscle. 2016;6:20 pubmed publisher
  4. Kaushik S, Cuervo A. Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis. Nat Cell Biol. 2015;17:759-70 pubmed publisher
  5. Zhong Y, Morris D, Jin L, Patel M, Karunakaran S, Fu Y, et al. Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem. 2014;289:26021-37 pubmed publisher