This is a Validated Antibody Database (VAD) review about human PICALM, based on 7 published articles (read how Labome selects the articles), using PICALM antibody in all methods. It is aimed to help Labome visitors find the most suited PICALM antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PICALM synonym: CALM; CLTH; LAP; phosphatidylinositol-binding clathrin assembly protein; clathrin assembly lymphoid myeloid leukemia protein

Knockout validation
Sigma-Aldrich
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
  • western blot knockout validation; mouse; 1:1000; fig 1
Sigma-Aldrich PICALM antibody (Sigma-Aldrich, HPA019061) was used in western blot on human samples at 1:1000 (fig 2) and in western blot knockout validation on mouse samples at 1:1000 (fig 1). PLoS ONE (2015) ncbi
Novus Biologicals
rabbit polyclonal
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 1
Novus Biologicals PICALM antibody (Novus Biologicals, NBP1-86658) was used in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 1). BMC Neurosci (2016) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • immunohistochemistry - paraffin section; dog; 1:200
LifeSpan Biosciences PICALM antibody (Lifespan, LS-B5065) was used in immunohistochemistry - paraffin section on dog samples at 1:200. Hum Genet (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunoprecipitation; mouse; loading ...; fig 8a
  • immunocytochemistry; mouse; 1:500; loading ...; fig 9b
  • western blot; mouse; 1:250; loading ...; fig 8a
Sigma-Aldrich PICALM antibody (Sigma, HPA019053) was used in immunoprecipitation on mouse samples (fig 8a), in immunocytochemistry on mouse samples at 1:500 (fig 9b) and in western blot on mouse samples at 1:250 (fig 8a). Brain Behav Immun (2018) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; fig 1
  • western blot; human; fig 4
In order to assess the correlation with levels of phosphotau and autophagy-related proteins and tau inclusions in PSP, Pick, and AD disease and levels of a key component of clathrin-mediated endocytosis, PICALM, Sigma-Aldrich PICALM antibody (Sigma-Aldrich, HPA019053) was used in immunohistochemistry - paraffin section on human samples (fig 1) and in western blot on human samples (fig 4). Neurobiol Dis (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; fig 3
In order to study the relationship between neurofibrillary tangles and raft domains, Sigma-Aldrich PICALM antibody (Sigma-Aldrich, HPA019053) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 3). Neuropathol Appl Neurobiol (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
  • western blot knockout validation; mouse; 1:1000; fig 1
Sigma-Aldrich PICALM antibody (Sigma-Aldrich, HPA019061) was used in western blot on human samples at 1:1000 (fig 2) and in western blot knockout validation on mouse samples at 1:1000 (fig 1). PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200; fig 5
  • western blot; human; 1:5000; fig 1
In order to determine the role of PICALM in Abeta transcytosis across the blood brain barrier, Sigma-Aldrich PICALM antibody (Sigma, HPA019061) was used in immunocytochemistry on human samples at 1:200 (fig 5) and in western blot on human samples at 1:5000 (fig 1). Nat Neurosci (2015) ncbi
Articles Reviewed
  1. Storck S, Hartz A, Bernard J, Wolf A, Kachlmeier A, Mahringer A, et al. The concerted amyloid-beta clearance of LRP1 and ABCB1/P-gp across the blood-brain barrier is linked by PICALM. Brain Behav Immun. 2018;73:21-33 pubmed publisher
  2. Thomas R, Henson A, Gerrish A, Jones L, Williams J, Kidd E. Decreasing the expression of PICALM reduces endocytosis and the activity of ?-secretase: implications for Alzheimer's disease. BMC Neurosci. 2016;17:50 pubmed publisher
  3. Ando K, Tomimura K, Sazdovitch V, Suain V, Yilmaz Z, Authelet M, et al. Level of PICALM, a key component of clathrin-mediated endocytosis, is correlated with levels of phosphotau and autophagy-related proteins and is associated with tau inclusions in AD, PSP and Pick disease. Neurobiol Dis. 2016;94:32-43 pubmed publisher
  4. Nishikawa T, Takahashi T, Nakamori M, Hosomi N, Maruyama H, Miyazaki Y, et al. The identification of raft-derived tau-associated vesicles that are incorporated into immature tangles and paired helical filaments. Neuropathol Appl Neurobiol. 2016;42:639-653 pubmed publisher
  5. Mercer J, Argus J, Crabtree D, KEENAN M, Wilks M, Chi J, et al. Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis. PLoS ONE. 2015;10:e0129776 pubmed publisher
  6. Zhao Z, Sagare A, Ma Q, Halliday M, Kong P, Kisler K, et al. Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance. Nat Neurosci. 2015;18:978-87 pubmed publisher
  7. Stern J, White S, Lehmkuhl L, Reina Doreste Y, Ferguson J, Nascone Yoder N, et al. A single codon insertion in PICALM is associated with development of familial subvalvular aortic stenosis in Newfoundland dogs. Hum Genet. 2014;133:1139-48 pubmed publisher