This is a Validated Antibody Database (VAD) review about human PIAS3, based on 11 published articles (read how Labome selects the articles), using PIAS3 antibody in all methods. It is aimed to help Labome visitors find the most suited PIAS3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PIAS3 synonym: ZMIZ5; E3 SUMO-protein ligase PIAS3; E3 SUMO-protein transferase PIAS3; protein inhibitor of activated STAT protein 3; zinc finger, MIZ-type containing 5

Invitrogen
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1g
  • western blot; mouse; loading ...; fig 1e
In order to investigate the contribution of protein inhibitor of activated STAT3 on atherogenesis, Invitrogen PIAS3 antibody (Thermo Fisher Scientific, PA5-20953) was used in immunohistochemistry - frozen section on mouse samples (fig 1g) and in western blot on mouse samples (fig 1e). Sci Rep (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; human; 1:1000; fig 4
In order to analyze multidrug resistant human cancer cells and autocrime cytokine/JAK/STAT-signaling induces kynurenin synthesis, Abcam PIAS3 antibody (Abcam, ab22856) was used in western blot on human samples at 1:1000 (fig 4). PLoS ONE (2015) ncbi
Epigentek
mouse monoclonal (4F12)
  • immunohistochemistry - paraffin section; human; 1:150; fig 2
Epigentek PIAS3 antibody (Epigentek, A-8151-100) was used in immunohistochemistry - paraffin section on human samples at 1:150 (fig 2). Int J Clin Exp Med (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-12)
  • western blot; human
Santa Cruz Biotechnology PIAS3 antibody (Santa Cruz, sc-46682) was used in western blot on human samples . J Biol Chem (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D5F9)
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology PIAS3 antibody (Cell Signaling, D5F9) was used in western blot on human samples (fig 1b). J Virol (2019) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig s2a
Cell Signaling Technology PIAS3 antibody (Cell Signaling, 4164) was used in western blot on rat samples at 1:1000 (fig s2a). Cell Death Differ (2017) ncbi
rabbit monoclonal (D5F9)
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology PIAS3 antibody (Cell Signaling, 9042) was used in western blot on human samples (fig 2a). Mol Oncol (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to investigate the effect of a microtubule inhibitor, MPT0B098, on STAT3 signaling in oral squamous cell carcinoma, Cell Signaling Technology PIAS3 antibody (Cell Signaling, 4164) was used in western blot on human samples (fig 4). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 8
Cell Signaling Technology PIAS3 antibody (Cell Signaling Tech, 4164) was used in western blot on human samples (fig 8). Oncotarget (2015) ncbi
rabbit monoclonal (D5F9)
  • western blot; human; fig 5
Cell Signaling Technology PIAS3 antibody (Cell signaling, 9042) was used in western blot on human samples (fig 5). BMC Genomics (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4
In order to determine the role of DBC1 sumoylation in p53-mediated apoptosis, Cell Signaling Technology PIAS3 antibody (Cell Signaling, 4164S) was used in western blot on human samples at 1:1000 (fig 4). Nat Commun (2014) ncbi
Articles Reviewed
  1. Liu S, Liu L, Xu G, Cao Z, Wang Q, Li S, et al. Epigenetic Modification Is Regulated by the Interaction of Influenza A Virus Nonstructural Protein 1 with the De Novo DNA Methyltransferase DNMT3B and Subsequent Transport to the Cytoplasm for K48-Linked Polyubiquitination. J Virol. 2019;93: pubmed publisher
  2. Tao C, Hsu W, Ma Y, Cheng S, Lee E. Epigenetic regulation of HDAC1 SUMOylation as an endogenous neuroprotection against Aβ toxicity in a mouse model of Alzheimer's disease. Cell Death Differ. 2017;24:597-614 pubmed publisher
  3. Zhang C, Mukherjee S, Tucker Burden C, Ross J, Chau M, Kong J, et al. TRIM8 regulates stemness in glioblastoma through PIAS3-STAT3. Mol Oncol. 2017;11:280-294 pubmed publisher
  4. Wang R, Zhang Y, Xu L, Lin Y, Yang X, Bai L, et al. Protein Inhibitor of Activated STAT3 Suppresses Oxidized LDL-induced Cell Responses during Atherosclerosis in Apolipoprotein E-deficient Mice. Sci Rep. 2016;6:36790 pubmed publisher
  5. Peng H, Cheng Y, Hsu Y, Wu G, Kuo C, Liou J, et al. MPT0B098, a Microtubule Inhibitor, Suppresses JAK2/STAT3 Signaling Pathway through Modulation of SOCS3 Stability in Oral Squamous Cell Carcinoma. PLoS ONE. 2016;11:e0158440 pubmed publisher
  6. Pitari M, Rossi M, Amodio N, Botta C, Morelli E, Federico C, et al. Inhibition of miR-21 restores RANKL/OPG ratio in multiple myeloma-derived bone marrow stromal cells and impairs the resorbing activity of mature osteoclasts. Oncotarget. 2015;6:27343-58 pubmed publisher
  7. Masotti A, Donninelli G, Da Sacco L, Varano B, Del Cornò M, Gessani S. HIV-1 gp120 influences the expression of microRNAs in human monocyte-derived dendritic cells via STAT3 activation. BMC Genomics. 2015;16:480 pubmed publisher
  8. Campia I, Buondonno I, Castella B, Rolando B, Kopecka J, Gazzano E, et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS ONE. 2015;10:e0126159 pubmed publisher
  9. Li J, Cui J, Zhang J, Liu Y, Han L, Jia C, et al. PIAS3, an inhibitor of STAT3, has intensively negative association with the survival of gastric cancer. Int J Clin Exp Med. 2015;8:682-9 pubmed
  10. Park J, Lee S, Yang S, Yoo H, Park J, Seong M, et al. Modification of DBC1 by SUMO2/3 is crucial for p53-mediated apoptosis in response to DNA damage. Nat Commun. 2014;5:5483 pubmed publisher
  11. Wang W, Chen Y, Wang S, Hu N, Cao Z, Wang W, et al. PIASx? ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase. J Biol Chem. 2014;289:3217-30 pubmed publisher