This is a Validated Antibody Database (VAD) review about human PHF1, based on 7 published articles (read how Labome selects the articles), using PHF1 antibody in all methods. It is aimed to help Labome visitors find the most suited PHF1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PHF1 synonym: MTF2L2; PCL1; PHF2; TDRD19C; hPHF1

Knockout validation
Abcam
domestic rabbit monoclonal (EPR14222)
  • western blot knockout validation; human; loading ...; fig 2b
  • immunohistochemistry - paraffin section; human; loading ...; fig 1c
Abcam PHF1 antibody (Abcam, ab184951) was used in western blot knockout validation on human samples (fig 2b) and in immunohistochemistry - paraffin section on human samples (fig 1c). Cell Death Discov (2022) ncbi
Abcam
domestic rabbit monoclonal (EPR14222)
  • western blot knockout validation; human; loading ...; fig 2b
  • immunohistochemistry - paraffin section; human; loading ...; fig 1c
Abcam PHF1 antibody (Abcam, ab184951) was used in western blot knockout validation on human samples (fig 2b) and in immunohistochemistry - paraffin section on human samples (fig 1c). Cell Death Discov (2022) ncbi
domestic rabbit monoclonal (EPR14222)
  • western blot; human; 1:1000; loading ...; fig 4b
Abcam PHF1 antibody (AbCam, Ab184951) was used in western blot on human samples at 1:1000 (fig 4b). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 2g
Abcam PHF1 antibody (Abcam, ab80042) was used in western blot on mouse samples at 1:1000 (fig 2g). CNS Neurosci Ther (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot; mouse; 1:200; loading ...; fig 3a
Santa Cruz Biotechnology PHF1 antibody (SCB, sc-515013) was used in western blot on mouse samples at 1:200 (fig 3a). Aging Cell (2019) ncbi
mouse monoclonal (864C6a)
  • western blot; human
In order to investigate the role of Rap1GAP in the cell cycle regulation, Santa Cruz Biotechnology PHF1 antibody (Santa Cruz Biotechnology, sc-130646) was used in western blot on human samples . PLoS ONE (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D45A2)
  • western blot; human; 1:1000; fig 3j
Cell Signaling Technology PHF1 antibody (Cell Signaling, 3497) was used in western blot on human samples at 1:1000 (fig 3j). Science (2016) ncbi
domestic rabbit monoclonal (D45A2)
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology PHF1 antibody (CST, D45A2) was used in western blot on human samples (fig 2c). J Biol Chem (2010) ncbi
Articles Reviewed
  1. Ning J, Wang F, Bu J, Zhu K, Liu W. Down-regulated m6A reader FTO destabilizes PHF1 that triggers enhanced stemness capacity and tumor progression in lung adenocarcinoma. Cell Death Discov. 2022;8:354 pubmed publisher
  2. Laliotis G, Chavdoula E, Paraskevopoulou M, Kaba A, La Ferlita A, Singh S, et al. AKT3-mediated IWS1 phosphorylation promotes the proliferation of EGFR-mutant lung adenocarcinomas through cell cycle-regulated U2AF2 RNA splicing. Nat Commun. 2021;12:4624 pubmed publisher
  3. di Meco A, Pratico D. Early-life exposure to high-fat diet influences brain health in aging mice. Aging Cell. 2019;18:e13040 pubmed publisher
  4. Zhang R, Liu Y, Chen Y, Li Q, Marshall C, Wu T, et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption. CNS Neurosci Ther. 2020;26:228-239 pubmed publisher
  5. Pattabiraman D, Bierie B, Kober K, Thiru P, Krall J, Zill C, et al. Activation of PKA leads to mesenchymal-to-epithelial transition and loss of tumor-initiating ability. Science. 2016;351:aad3680 pubmed publisher
  6. Wang D, Zhang P, Gao K, Tang Y, Jin X, Zhang Y, et al. PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation. PLoS ONE. 2014;9:e110296 pubmed publisher
  7. Wen H, Li J, Song T, Lu M, Kan P, Lee M, et al. Recognition of histone H3K4 trimethylation by the plant homeodomain of PHF2 modulates histone demethylation. J Biol Chem. 2010;285:9322-6 pubmed publisher