This is a Validated Antibody Database (VAD) review about human PFN1, based on 7 published articles (read how Labome selects the articles), using PFN1 antibody in all methods. It is aimed to help Labome visitors find the most suited PFN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PFN1 synonym: ALS18

Knockout validation
Abcam
domestic rabbit monoclonal (EPR6304)
  • immunocytochemistry; human; 1:1000; loading ...; fig 4a, s5a
  • western blot; human; loading ...; fig 4b
  • western blot knockout validation; mouse; loading ...; fig s5h
Abcam PFN1 antibody (Abcam, ab124904) was used in immunocytochemistry on human samples at 1:1000 (fig 4a, s5a), in western blot on human samples (fig 4b) and in western blot knockout validation on mouse samples (fig s5h). Cell Rep (2021) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s5h
  • western blot; human; loading ...; fig 4b
Cell Signaling Technology PFN1 antibody (Cell Signaling, 3237) was used in western blot knockout validation on mouse samples (fig s5h) and in western blot on human samples (fig 4b). Cell Rep (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR6304)
  • immunohistochemistry - paraffin section; human; 1:250; loading ...; fig 5bi
  • western blot; human; 1:10,000; loading ...; fig 5ai
Abcam PFN1 antibody (Abcam, ab124904) was used in immunohistochemistry - paraffin section on human samples at 1:250 (fig 5bi) and in western blot on human samples at 1:10,000 (fig 5ai). Int J Mol Sci (2022) ncbi
domestic rabbit monoclonal (EPR6304)
  • immunocytochemistry; human; 1:1000; loading ...; fig 4a, s5a
  • western blot; human; loading ...; fig 4b
  • western blot knockout validation; mouse; loading ...; fig s5h
Abcam PFN1 antibody (Abcam, ab124904) was used in immunocytochemistry on human samples at 1:1000 (fig 4a, s5a), in western blot on human samples (fig 4b) and in western blot knockout validation on mouse samples (fig s5h). Cell Rep (2021) ncbi
domestic rabbit monoclonal (EPR6304)
  • immunohistochemistry - paraffin section; fruit fly ; 1:100; loading ...
Abcam PFN1 antibody (Abcam, ab124904) was used in immunohistochemistry - paraffin section on fruit fly samples at 1:100. J Biol Chem (2016) ncbi
domestic rabbit monoclonal (EPR6304)
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1
In order to assess the prognostic value of an immunohistochemistry-based three-protein signature model in patients with esophageal squamous cell carcinoma, Abcam PFN1 antibody (Abcam, ab124904) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1). Oncotarget (2015) ncbi
Novus Biologicals
mouse monoclonal (OTI1D5)
  • immunocytochemistry; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4a
Novus Biologicals PFN1 antibody (Novus Biologicals, NBP2-02577) was used in immunocytochemistry on human samples (fig 4b) and in western blot on human samples (fig 4a). EMBO J (2019) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 7d
Cell Signaling Technology PFN1 antibody (Cell Signalling, 3237) was used in western blot on mouse samples at 1:1000 (fig 7d). Nat Commun (2022) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s5h
  • western blot; human; loading ...; fig 4b
Cell Signaling Technology PFN1 antibody (Cell Signaling, 3237) was used in western blot knockout validation on mouse samples (fig s5h) and in western blot on human samples (fig 4b). Cell Rep (2021) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1a
  • western blot; mouse; 1:100; loading ...; fig 7c
MilliporeSigma PFN1 antibody (Abcam, P7749) was used in immunohistochemistry on mouse samples at 1:100 (fig 1a) and in western blot on mouse samples at 1:100 (fig 7c). Mol Biol Cell (2016) ncbi
Articles Reviewed
  1. Lohraseb I, McCarthy P, Secker G, Marchant C, Wu J, Ali N, et al. Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells. Nat Commun. 2022;13:2018 pubmed publisher
  2. Nishtala K, Panigrahi T, Shetty R, Kumar D, Khamar P, Mohan R, et al. Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing. Int J Mol Sci. 2022;23: pubmed publisher
  3. Zhu C, Kim S, Mooradian A, Wang F, Li Z, Holohan S, et al. Cancer-associated exportin-6 upregulation inhibits the transcriptionally repressive and anticancer effects of nuclear profilin-1. Cell Rep. 2021;34:108749 pubmed publisher
  4. Chatzifrangkeskou M, Pefani D, Eyres M, Vendrell I, Fischer R, Panková D, et al. RASSF1A is required for the maintenance of nuclear actin levels. EMBO J. 2019;38:e101168 pubmed publisher
  5. Matsukawa K, Hashimoto T, Matsumoto T, Ihara R, Chihara T, Miura M, et al. Familial Amyotrophic Lateral Sclerosis-linked Mutations in Profilin 1 Exacerbate TDP-43-induced Degeneration in the Retina of Drosophila melanogaster through an Increase in the Cytoplasmic Localization of TDP-43. J Biol Chem. 2016;291:23464-23476 pubmed
  6. Nejedlá M, Sadi S, Sulimenko V, de Almeida F, Blom H, Draber P, et al. Profilin connects actin assembly with microtubule dynamics. Mol Biol Cell. 2016;27:2381-93 pubmed publisher
  7. Cao H, Zhang S, SHEN J, Wu Z, Wu J, Wang S, et al. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 2015;6:5435-48 pubmed