This is a Validated Antibody Database (VAD) review about human PFKFB4, based on 7 published articles (read how Labome selects the articles), using PFKFB4 antibody in all methods. It is aimed to help Labome visitors find the most suited PFKFB4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Abcam
domestic rabbit monoclonal (EPR12594)
  • western blot knockout validation; human; 1:3000; loading ...; fig 1d
  • immunoprecipitation; human; 1:3000; loading ...; fig 1f
Abcam PFKFB4 antibody (Abcam, ab181861) was used in western blot knockout validation on human samples at 1:3000 (fig 1d) and in immunoprecipitation on human samples at 1:3000 (fig 1f). Nat Commun (2018) ncbi
Abcam
domestic rabbit monoclonal (EPR12594)
  • western blot; human; 1:2000; loading ...; fig 2e
Abcam PFKFB4 antibody (Abcam, ab181861) was used in western blot on human samples at 1:2000 (fig 2e). J Cancer (2021) ncbi
domestic rabbit monoclonal (EPR12594)
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig s2
  • western blot; mouse; 1:1000; fig 3a
Abcam PFKFB4 antibody (Abcam, ab181861) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig s2) and in western blot on mouse samples at 1:1000 (fig 3a). Aging (Albany NY) (2021) ncbi
domestic rabbit monoclonal (EPR12594)
  • immunohistochemistry - paraffin section; African green monkey; 10 ug/ml; loading ...; fig 4f
  • western blot; African green monkey; 1:1000; loading ...; fig 4i
  • immunocytochemistry; human; 1:100; loading ...; fig 5h
  • western blot; human; 1:1000; loading ...; fig 5j
  • immunocytochemistry; rat; 1:100; loading ...; fig 7e
Abcam PFKFB4 antibody (Abcam, ab181861) was used in immunohistochemistry - paraffin section on African green monkey samples at 10 ug/ml (fig 4f), in western blot on African green monkey samples at 1:1000 (fig 4i), in immunocytochemistry on human samples at 1:100 (fig 5h), in western blot on human samples at 1:1000 (fig 5j) and in immunocytochemistry on rat samples at 1:100 (fig 7e). Commun Biol (2021) ncbi
domestic rabbit monoclonal (EPR12594)
  • immunoprecipitation; human; 1:50; loading ...; fig 4h
  • immunohistochemistry; human; 1:250; loading ...; fig 6d
  • western blot; human; 1:1000; loading ...; fig 3f
Abcam PFKFB4 antibody (Abcam, ab181861) was used in immunoprecipitation on human samples at 1:50 (fig 4h), in immunohistochemistry on human samples at 1:250 (fig 6d) and in western blot on human samples at 1:1000 (fig 3f). Nat Commun (2020) ncbi
domestic rabbit monoclonal (EPR12594)
  • western blot knockout validation; human; 1:3000; loading ...; fig 1d
  • immunoprecipitation; human; 1:3000; loading ...; fig 1f
Abcam PFKFB4 antibody (Abcam, ab181861) was used in western blot knockout validation on human samples at 1:3000 (fig 1d) and in immunoprecipitation on human samples at 1:3000 (fig 1f). Nat Commun (2018) ncbi
domestic rabbit monoclonal (EPR12594)
  • western blot; mouse; loading ...; fig 1a
Abcam PFKFB4 antibody (Abcam, ab181861) was used in western blot on mouse samples (fig 1a). J Mol Cell Cardiol (2017) ncbi
domestic rabbit monoclonal (EPR12594)
  • western blot; human; loading ...; fig 8a
Abcam PFKFB4 antibody (Abcam, ab181861) was used in western blot on human samples (fig 8a). Cell Signal (2017) ncbi
Articles Reviewed
  1. Li H, Shen H, Xie P, Zhang Z, Wang L, Yang Y, et al. Role of long intergenic non-protein coding RNA 00152 in pancreatic cancer glycolysis via the manipulation of the microRNA-185-5p/Krüppel-like factor 7 axis. J Cancer. 2021;12:6330-6343 pubmed publisher
  2. Pan W, Song X, Hu Q, Zhang Y. miR-485 inhibits histone deacetylase HDAC5, HIF1α and PFKFB3 expression to alleviate epilepsy in cellular and rodent models. Aging (Albany NY). 2021;13:14416-14432 pubmed publisher
  3. Liu M, Li N, Qu C, Gao Y, Wu L, Hu L. Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Commun Biol. 2021;4:188 pubmed publisher
  4. Liu J, Liu Z, Wu Q, Lu Y, Wong C, Miao L, et al. Long noncoding RNA AGPG regulates PFKFB3-mediated tumor glycolytic reprogramming. Nat Commun. 2020;11:1507 pubmed publisher
  5. Li F, Liu J, Bao R, Yan G, Feng X, Xu Y, et al. Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis. Nat Commun. 2018;9:508 pubmed publisher
  6. He X, Zeng H, Chen S, Roman R, Aschner J, Didion S, et al. Endothelial specific SIRT3 deletion impairs glycolysis and angiogenesis and causes diastolic dysfunction. J Mol Cell Cardiol. 2017;112:104-113 pubmed publisher
  7. Houddane A, Bultot L, Novellasdemunt L, Johanns M, Gueuning M, Vertommen D, et al. Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes. Cell Signal. 2017;34:23-37 pubmed publisher