This is a Validated Antibody Database (VAD) review about human PEDF, based on 5 published articles (read how Labome selects the articles), using PEDF antibody in all methods. It is aimed to help Labome visitors find the most suited PEDF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PEDF synonym: EPC-1; OI12; OI6; PEDF; PIG35; pigment epithelium-derived factor; alpha-2 antiplasmin; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; testis tissue sperm-binding protein Li 70n

Abcam
rabbit monoclonal (EPR9826(B))
  • western blot; human; loading ...; fig s10c
In order to discuss how signal peptidase complex proteins affect flaviviruses, Abcam PEDF antibody (Abcam, ab157207) was used in western blot on human samples (fig s10c). Nature (2016) ncbi
rabbit monoclonal (EPR9826(B))
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 6a
  • western blot; human; 1:1000; loading ...; fig 5b
Abcam PEDF antibody (Abcam, ab157207) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 6a) and in western blot on human samples at 1:1000 (fig 5b). Cancer Biol Ther (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Abcam PEDF antibody (Abcam, ab180711) was used in western blot on human samples at 1:1000. Mol Cell Biochem (2015) ncbi
R&D Systems
goat polyclonal
  • western blot; human; loading ...; fig 6a
R&D Systems PEDF antibody (R & D systems, AF1177) was used in western blot on human samples (fig 6a). Int J Radiat Biol (2017) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; 1:1000
  • immunohistochemistry; mouse; 1:100
In order to study the role of perivascular macrophage-like melanocyte in acoustic trauma and hearing loss, EMD Millipore PEDF antibody (Millipore, 07-280) was used in western blot on human samples at 1:1000 and in immunohistochemistry on mouse samples at 1:100. FASEB J (2013) ncbi
Articles Reviewed
  1. Yentrapalli R, Merl Pham J, Azimzadeh O, Mutschelknaus L, Peters C, Hauck S, et al. Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation. Int J Radiat Biol. 2017;93:569-580 pubmed publisher
  2. Zhang R, Miner J, Gorman M, Rausch K, Ramage H, White J, et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature. 2016;535:164-8 pubmed
  3. Chen X, Li C, He T, Mao J, Li C, Lyu J, et al. Metformin inhibits prostate cancer cell proliferation, migration, and tumor growth through upregulation of PEDF expression. Cancer Biol Ther. 2016;17:507-14 pubmed publisher
  4. Zhang J, Zhao J, Bai Y, Huang L, Yu W, Li X. Effects of p75 neurotrophin receptor on regulating hypoxia-induced angiogenic factors in retinal pigment epithelial cells. Mol Cell Biochem. 2015;398:123-34 pubmed publisher
  5. Zhang F, Dai M, Neng L, Zhang J, Zhi Z, Fridberger A, et al. Perivascular macrophage-like melanocyte responsiveness to acoustic trauma--a salient feature of strial barrier associated hearing loss. FASEB J. 2013;27:3730-40 pubmed publisher