This is a Validated Antibody Database (VAD) review about human PDX-1, based on 27 published articles (read how Labome selects the articles), using PDX-1 antibody in all methods. It is aimed to help Labome visitors find the most suited PDX-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PDX-1 synonym: GSF; IDX-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; STF-1

Abcam
domestic rabbit monoclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 4g
Abcam PDX-1 antibody (Abcam, ab134150) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 4g). iScience (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5e
Abcam PDX-1 antibody (Abcam, ab47267) was used in immunohistochemistry - paraffin section on mouse samples (fig 5e). Oncogene (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 6c
Abcam PDX-1 antibody (Abcam, ab47267) was used in immunohistochemistry - paraffin section on mouse samples at 1:400 (fig 6c). Int J Mol Sci (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig s4b
Abcam PDX-1 antibody (Abcam, 47267) was used in immunohistochemistry on mouse samples (fig s4b). Cell Rep (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:5000; loading ...; fig 6b
Abcam PDX-1 antibody (Abcam, ab47267) was used in immunohistochemistry - frozen section on mouse samples at 1:5000 (fig 6b). Cell Death Discov (2021) ncbi
domestic rabbit monoclonal (EPR22002)
  • immunocytochemistry; mouse; loading ...; fig 5b
  • western blot; mouse; loading ...; fig 5c
Abcam PDX-1 antibody (Abcam, ab219207) was used in immunocytochemistry on mouse samples (fig 5b) and in western blot on mouse samples (fig 5c). Cell Rep (2021) ncbi
guinea-pigs polyclonal
  • immunohistochemistry; mouse; loading ...; fig 2o
Abcam PDX-1 antibody (Abcam, ab47308) was used in immunohistochemistry on mouse samples (fig 2o). Cell Rep (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:300; loading ...; fig 8a
Abcam PDX-1 antibody (Abcam, ab47267) was used in immunohistochemistry - paraffin section on mouse samples at 1:300 (fig 8a). Cells (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 5c
Abcam PDX-1 antibody (Abcam, ab47267) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000 (fig 5c). Nat Commun (2020) ncbi
domestic goat polyclonal
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1a
Abcam PDX-1 antibody (Abcam, ab47383) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1a). Stem Cells Transl Med (2019) ncbi
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:5000; loading ...; fig e5h
Abcam PDX-1 antibody (Abcam, ab47383) was used in immunohistochemistry - paraffin section on mouse samples at 1:5000 (fig e5h). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 7f
Abcam PDX-1 antibody (Abcam, ab47267) was used in western blot on human samples (fig 7f). Nucleic Acids Res (2019) ncbi
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:5000; loading ...; fig s1a
Abcam PDX-1 antibody (Abcam, ab47383) was used in immunohistochemistry - paraffin section on mouse samples at 1:5000 (fig s1a). Nature (2017) ncbi
domestic goat polyclonal
  • immunohistochemistry; mouse; loading ...; fig 2
In order to compare the effects of losartan and telmisartan on pancreatic islets remodeling and glucose homeostasis in diet-induced obese mice, Abcam PDX-1 antibody (Abcam, ab47383) was used in immunohistochemistry on mouse samples (fig 2). Mol Cell Endocrinol (2017) ncbi
guinea-pigs polyclonal
  • flow cytometry; human; 1:10,000; loading ...; tbl 1
  • immunocytochemistry; human; 1:10,000; loading ...; tbl 1
In order to describe a method to produce human embryonic stem cell-derived Beta-like cells, Abcam PDX-1 antibody (Abcam, ab47308) was used in flow cytometry on human samples at 1:10,000 (tbl 1) and in immunocytochemistry on human samples at 1:10,000 (tbl 1). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; hamsters; fig 4
  • western blot; hamsters; fig 5
  • western blot; rat; fig 1
Abcam PDX-1 antibody (Abcam, ab47267) was used in chromatin immunoprecipitation on hamsters samples (fig 4), in western blot on hamsters samples (fig 5) and in western blot on rat samples (fig 1). Mol Cell Endocrinol (2016) ncbi
domestic rabbit polyclonal
In order to examine the role of the LIM homeodomain transcription factor Isl1 in pyloric development, Abcam PDX-1 antibody (Abcam, ab47267) was used . BMC Biol (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-11)
  • western blot; mouse; 1:200; loading ...; fig 2g
Santa Cruz Biotechnology PDX-1 antibody (Santa Cruz, sc-390792) was used in western blot on mouse samples at 1:200 (fig 2g). FASEB J (2017) ncbi
mouse monoclonal (E-5)
  • immunocytochemistry; human; fig 6g
  • western blot; human; fig 6d
Santa Cruz Biotechnology PDX-1 antibody (SantaCruz, sc-390808) was used in immunocytochemistry on human samples (fig 6g) and in western blot on human samples (fig 6d). Sci Rep (2014) ncbi
R&D Systems
domestic goat polyclonal
  • immunocytochemistry; human; 1:250; fig 2d
R&D Systems PDX-1 antibody (R&D Systems, AF2419) was used in immunocytochemistry on human samples at 1:250 (fig 2d). Diabetes (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D59H3)
  • western blot; mouse; loading ...; fig s11a
Cell Signaling Technology PDX-1 antibody (Cell Signalling, 5679) was used in western blot on mouse samples (fig s11a). Oncogene (2022) ncbi
domestic rabbit monoclonal (D59H3)
  • immunocytochemistry; mouse; loading ...; fig 5b
  • western blot; mouse; loading ...; fig 5c
Cell Signaling Technology PDX-1 antibody (Cell Signaling Technology, 5679) was used in immunocytochemistry on mouse samples (fig 5b) and in western blot on mouse samples (fig 5c). Cell Rep (2021) ncbi
domestic rabbit monoclonal (D59H3)
  • immunocytochemistry; mouse; loading ...; fig 3c
Cell Signaling Technology PDX-1 antibody (Cell Signaling, 5679) was used in immunocytochemistry on mouse samples (fig 3c). J Clin Invest (2019) ncbi
domestic rabbit monoclonal (D59H3)
  • immunocytochemistry; human; fig 2a
  • western blot; human; fig 2d
Cell Signaling Technology PDX-1 antibody (Cell Signaling, 5679S) was used in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 2d). Cell (2018) ncbi
domestic rabbit monoclonal (D59H3)
  • western blot; rat; 1:1000; loading ...; fig s3b
Cell Signaling Technology PDX-1 antibody (Cell Signaling, 5679) was used in western blot on rat samples at 1:1000 (fig s3b). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D59H3)
  • immunohistochemistry - paraffin section; human; 1:100; fig 2
In order to analyze a model for ductal pancreatic cancer and drug screening, Cell Signaling Technology PDX-1 antibody (Cell Signaling Technology, 5679) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 2). Nat Med (2015) ncbi
BD Biosciences
mouse monoclonal (658A5)
  • flow cytometry; human; fig 3b
BD Biosciences PDX-1 antibody (BD Biosciences, 562161) was used in flow cytometry on human samples (fig 3b). Dev Cell (2022) ncbi
mouse monoclonal (658A5)
  • flow cytometry; human; 1:10; loading ...; fig 4s1c
BD Biosciences PDX-1 antibody (BD Biosciences, 562161) was used in flow cytometry on human samples at 1:10 (fig 4s1c). elife (2021) ncbi
mouse monoclonal (658A5)
  • flow cytometry; human; 1:80; fig 2b, s3a
BD Biosciences PDX-1 antibody (BD Biosciences, 562161) was used in flow cytometry on human samples at 1:80 (fig 2b, s3a). Diabetes (2021) ncbi
mouse monoclonal (658A5)
  • flow cytometry; human; 1:40; fig s1
In order to assess the impact of thyroid dysregulation on the development of human embryonic stem cells-derived progenitor cells in vivo, BD Biosciences PDX-1 antibody (BD, 562161) was used in flow cytometry on human samples at 1:40 (fig s1). Diabetes (2016) ncbi
Articles Reviewed
  1. Chen K, Zhang J, Huang Y, Tian X, Yang Y, Dong A. Single-cell RNA-seq transcriptomic landscape of human and mouse islets and pathological alterations of diabetes. iScience. 2022;25:105366 pubmed publisher
  2. Miguel Escalada I, Maestro M, Balboa D, Elek A, Bernal A, Bernardo E, et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster. Dev Cell. 2022;57:1922-1936.e9 pubmed publisher
  3. Kidger A, Saville M, Rushworth L, Davidson J, Stellzig J, Ono M, et al. Suppression of mutant Kirsten-RAS (KRASG12D)-driven pancreatic carcinogenesis by dual-specificity MAP kinase phosphatases 5 and 6. Oncogene. 2022;41:2811-2823 pubmed publisher
  4. Nahle A, Joseph Y, Pereira S, Mori Y, Poon F, Ghadieh H, et al. Nicotinamide Mononucleotide Prevents Free Fatty Acid-Induced Reduction in Glucose Tolerance by Decreasing Insulin Clearance. Int J Mol Sci. 2021;22: pubmed publisher
  5. Lupse B, Annamalai K, Ibrahim H, Kaur S, Geravandi S, Sarma B, et al. Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes. Cell Rep. 2021;36:109490 pubmed publisher
  6. Lasierra Losada M, Pauler M, Vandamme N, Goossens S, Berx G, Leppkes M, et al. Pancreas morphogenesis and homeostasis depends on tightly regulated Zeb1 levels in epithelial cells. Cell Death Discov. 2021;7:138 pubmed publisher
  7. Zhang X, Wang X, Yuan Z, Radford S, Liu C, Libutti S, et al. Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis. Cell Rep. 2021;34:108830 pubmed publisher
  8. Geusz R, Wang A, Chiou J, Lancman J, Wetton N, Kefalopoulou S, et al. Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in development. elife. 2021;10: pubmed publisher
  9. Montaser H, Patel K, Balboa D, Ibrahim H, Lithovius V, Näätänen A, et al. Loss of MANF Causes Childhood-Onset Syndromic Diabetes Due to Increased Endoplasmic Reticulum Stress. Diabetes. 2021;70:1006-1018 pubmed publisher
  10. Viloria K, Nasteska D, Briant L, Heising S, Larner D, Fine N, et al. Vitamin-D-Binding Protein Contributes to the Maintenance of α Cell Function and Glucagon Secretion. Cell Rep. 2020;31:107761 pubmed publisher
  11. Pittala S, Levy I, De S, Kumar Pandey S, Melnikov N, Hyman T, et al. The VDAC1-based R-Tf-D-LP4 Peptide as a Potential Treatment for Diabetes Mellitus. Cells. 2020;9: pubmed publisher
  12. Zhu K, Lai Y, Cao H, Bai X, Liu C, Yan Q, et al. Kindlin-2 modulates MafA and β-catenin expression to regulate β-cell function and mass in mice. Nat Commun. 2020;11:484 pubmed publisher
  13. Villani V, Thornton M, Zook H, Crook C, Grubbs B, Orlando G, et al. SOX9+/PTF1A+ Cells Define the Tip Progenitor Cells of the Human Fetal Pancreas of the Second Trimester. Stem Cells Transl Med. 2019;8:1249-1264 pubmed publisher
  14. Shi Y, Gao W, Lytle N, Huang P, Yuan X, Dann A, et al. Targeting LIF-mediated paracrine interaction for pancreatic cancer therapy and monitoring. Nature. 2019;569:131-135 pubmed publisher
  15. Haldeman J, Conway A, Arlotto M, Slentz D, Muoio D, Becker T, et al. Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res. 2019;47:e23 pubmed publisher
  16. Bartolomé A, Zhu C, Sussel L, Pajvani U. Notch signaling dynamically regulates adult β cell proliferation and maturity. J Clin Invest. 2019;129:268-280 pubmed publisher
  17. Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  18. Wei J, Ding D, Wang T, Liu Q, Lin Y. MiR-338 controls BPA-triggered pancreatic islet insulin secretory dysfunction from compensation to decompensation by targeting Pdx-1. FASEB J. 2017;31:5184-5195 pubmed publisher
  19. Axelsson A, Mahdi T, Nenonen H, Singh T, Hänzelmann S, Wendt A, et al. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes. Nat Commun. 2017;8:15652 pubmed publisher
  20. McCracken K, Aihara E, Martin B, Crawford C, Broda T, Treguier J, et al. Wnt/β-catenin promotes gastric fundus specification in mice and humans. Nature. 2017;541:182-187 pubmed publisher
  21. Graus Nunes F, Marinho T, Barbosa da Silva S, Aguila M, Mandarim de Lacerda C, Souza Mello V. Differential effects of angiotensin receptor blockers on pancreatic islet remodelling and glucose homeostasis in diet-induced obese mice. Mol Cell Endocrinol. 2017;439:54-64 pubmed publisher
  22. Massumi M, Pourasgari F, Nalla A, Batchuluun B, Nagy K, Neely E, et al. An Abbreviated Protocol for In Vitro Generation of Functional Human Embryonic Stem Cell-Derived Beta-Like Cells. PLoS ONE. 2016;11:e0164457 pubmed publisher
  23. Wang W, Shi Q, Guo T, Yang Z, Jia Z, Chen P, et al. PDX1 and ISL1 differentially coordinate with epigenetic modifications to regulate insulin gene expression in varied glucose concentrations. Mol Cell Endocrinol. 2016;428:38-48 pubmed publisher
  24. Bruin J, Saber N, O Dwyer S, Fox J, Mojibian M, Arora P, et al. Hypothyroidism Impairs Human Stem Cell-Derived Pancreatic Progenitor Cell Maturation in Mice. Diabetes. 2016;65:1297-309 pubmed publisher
  25. Huang L, Holtzinger A, Jagan I, BeGora M, Lohse I, Ngai N, et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med. 2015;21:1364-71 pubmed publisher
  26. Pettinato G, Wen X, Zhang N. Formation of well-defined embryoid bodies from dissociated human induced pluripotent stem cells using microfabricated cell-repellent microwell arrays. Sci Rep. 2014;4:7402 pubmed publisher
  27. Li Y, Pan J, Wei C, Chen J, Liu Y, Liu J, et al. LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3. BMC Biol. 2014;12:25 pubmed publisher