This is a Validated Antibody Database (VAD) review about human PCM1, based on 16 published articles (read how Labome selects the articles), using PCM1 antibody in all methods. It is aimed to help Labome visitors find the most suited PCM1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PCM1 synonym: PTC4; RET/PCM-1; pericentriolar material 1 protein; PCM-1; hPCM-1; pericentriolar material 1, PCM1

Santa Cruz Biotechnology
mouse monoclonal
  • immunocytochemistry; human; loading ...; fig 4a
Santa Cruz Biotechnology PCM1 antibody (SantaCruz, sc-398365) was used in immunocytochemistry on human samples (fig 4a). J Biol Chem (2017) ncbi
mouse monoclonal
  • immunocytochemistry; human; 1:200; loading ...; fig 2c
In order to show that USP9X regulates centrosome biogenesis and plays a role breast carcinogenesis, Santa Cruz Biotechnology PCM1 antibody (Santa Cruz, sc-398365) was used in immunocytochemistry on human samples at 1:200 (fig 2c). Nat Commun (2017) ncbi
Abcam
mouse monoclonal (CL0206)
  • immunocytochemistry; human; 1:1000; fig 7
  • western blot; human; 1:1000; fig 1
Abcam PCM1 antibody (Abcam, ab154142) was used in immunocytochemistry on human samples at 1:1000 (fig 7) and in western blot on human samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; human
In order to identify and characterize the TALPID3 (KIAA0586) mutation in patients with Joubert syndrome, Abcam PCM1 antibody (Abcam, ab72443) was used in immunohistochemistry on human samples . elife (2015) ncbi
Bethyl
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; fig 1
  • immunohistochemistry; human; 1:1000; fig 1
Bethyl PCM1 antibody (Bethyl Laboratories, A301-150A) was used in immunocytochemistry on human samples at 1:1000 (fig 1) and in immunohistochemistry on human samples at 1:1000 (fig 1). Oncotarget (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:350; fig 1
  • western blot; human; 1:1000; fig 5
Bethyl PCM1 antibody (Bethyl, A301-150A) was used in immunocytochemistry on human samples at 1:350 (fig 1) and in western blot on human samples at 1:1000 (fig 5). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:500; fig 10
Bethyl PCM1 antibody (Bethyl, A301-149A) was used in western blot on human samples at 1:500 (fig 10). Nature (2015) ncbi
Proteintech Group
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 2a
Proteintech Group PCM1 antibody (Proteintech, 19856-1-AP) was used in immunoprecipitation on human samples (fig 5a) and in western blot on human samples (fig 2a). J Biol Chem (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig s4d
In order to show that USP9X regulates centrosome biogenesis and plays a role breast carcinogenesis, Proteintech Group PCM1 antibody (Proteintech, 19856-1-AP) was used in western blot on human samples at 1:1000 (fig s4d). Nat Commun (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6a
Proteintech Group PCM1 antibody (Proteintech, 19856-1-AP) was used in western blot on human samples at 1:1000 (fig 6a). J Cell Sci (2017) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 3c
Cell Signaling Technology PCM1 antibody (Cell Signaling Technology, 5259S) was used in immunocytochemistry on human samples (fig 3c). Nature (2018) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 2c
In order to Investigate the regulation of GABARAP ubiquitination and GABARAP-mediated autophagy, Cell Signaling Technology PCM1 antibody (Cell signaling, 5213) was used in immunocytochemistry on human samples (fig 2c). Curr Biol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:400; fig s3
Cell Signaling Technology PCM1 antibody (Cell Signaling Tech, 5259) was used in immunocytochemistry on human samples at 1:400 (fig s3). J Cell Biol (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human
In order to investigate centrosome regulation during fever, Cell Signaling Technology PCM1 antibody (Cell Signaling, 5213) was used in immunocytochemistry on human samples . Mol Biol Cell (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human
Cell Signaling Technology PCM1 antibody (Cell Signaling, 5259) was used in immunocytochemistry on human samples . Am J Hum Genet (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunohistochemistry; human; 1:500; loading ...; fig s2c
Sigma-Aldrich PCM1 antibody (Sigma-Aldrich, HPA023370) was used in immunohistochemistry on human samples at 1:500 (fig s2c). Cell Rep (2018) ncbi
mouse monoclonal
  • immunocytochemistry; human; loading ...; fig 2a
In order to Investigate the regulation of GABARAP ubiquitination and GABARAP-mediated autophagy, Sigma-Aldrich PCM1 antibody (Sigma Aldrich, SAB1406228) was used in immunocytochemistry on human samples (fig 2a). Curr Biol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:300; fig s5j
Sigma-Aldrich PCM1 antibody (Sigma, HPA023370) was used in immunocytochemistry on human samples at 1:300 (fig s5j). EMBO J (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 1a
In order to explore the underlying mechanisms of the variable expressivity of CEP290 in ciliopathies, Sigma-Aldrich PCM1 antibody (Sigma, HPA023374) was used in western blot on human samples (fig 1a). Hum Mol Genet (2014) ncbi
Articles Reviewed
  1. Rai A, Chen J, Selbach M, Pelkmans L. Kinase-controlled phase transition of membraneless organelles in mitosis. Nature. 2018;559:211-216 pubmed publisher
  2. Mönnich M, Borgeskov L, Breslin L, Jakobsen L, Rogowski M, Doğanlı C, et al. CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium. Cell Rep. 2018;22:2584-2592 pubmed publisher
  3. Joachim J, Razi M, Judith D, Wirth M, Calamita E, Encheva V, et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr Biol. 2017;27:2123-2136.e7 pubmed publisher
  4. Wang Q, Tang Y, Xu Y, Xu S, Jiang Y, Dong Q, et al. The X-linked deubiquitinase USP9X is an integral component of centrosome. J Biol Chem. 2017;292:12874-12884 pubmed publisher
  5. Li X, Song N, Liu L, Liu X, Ding X, Song X, et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun. 2017;8:14866 pubmed publisher
  6. Conkar D, Culfa E, Odabasi E, Rauniyar N, Yates J, Firat Karalar E. The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. J Cell Sci. 2017;130:1450-1462 pubmed publisher
  7. Meitinger F, Anzola J, Kaulich M, Richardson A, Stender J, Benner C, et al. 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 2016;214:155-66 pubmed publisher
  8. Chavali P, Chandrasekaran G, Barr A, Tátrai P, Taylor C, Papachristou E, et al. A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun. 2016;7:11005 pubmed publisher
  9. Hoang Minh L, Deleyrolle L, Siebzehnrubl D, Ugartemendia G, Futch H, Griffith B, et al. Disruption of KIF3A in patient-derived glioblastoma cells: effects on ciliogenesis, hedgehog sensitivity, and tumorigenesis. Oncotarget. 2016;7:7029-43 pubmed publisher
  10. Tollenaere M, Villumsen B, Blasius M, Nielsen J, Wagner S, Bartek J, et al. p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nat Commun. 2015;6:10075 pubmed publisher
  11. Werner A, Iwasaki S, McGourty C, Medina Ruiz S, Teerikorpi N, Fedrigo I, et al. Cell-fate determination by ubiquitin-dependent regulation of translation. Nature. 2015;525:523-7 pubmed publisher
  12. Stephen L, Tawamie H, Davis G, Tebbe L, Nürnberg P, Nürnberg G, et al. TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23). elife. 2015;4: pubmed publisher
  13. Vertii A, Zimmerman W, Ivshina M, Doxsey S. Centrosome-intrinsic mechanisms modulate centrosome integrity during fever. Mol Biol Cell. 2015;26:3451-63 pubmed publisher
  14. Schueler M, Braun D, Chandrasekar G, Gee H, Klasson T, Halbritter J, et al. DCDC2 mutations cause a renal-hepatic ciliopathy by disrupting Wnt signaling. Am J Hum Genet. 2015;96:81-92 pubmed publisher
  15. Shiratsuchi G, Takaoka K, Ashikawa T, Hamada H, Kitagawa D. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J. 2015;34:97-114 pubmed publisher
  16. Zhang Y, Seo S, Bhattarai S, Bugge K, Searby C, Zhang Q, et al. BBS mutations modify phenotypic expression of CEP290-related ciliopathies. Hum Mol Genet. 2014;23:40-51 pubmed publisher