This is a Validated Antibody Database (VAD) review about human PCAF, based on 28 published articles (read how Labome selects the articles), using PCAF antibody in all methods. It is aimed to help Labome visitors find the most suited PCAF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PCAF synonym: CAF; P/CAF; PCAF

Santa Cruz Biotechnology
mouse monoclonal (E-8)
  • western blot; human; loading ...; fig 5b
  • western blot; mouse; loading ...; fig s8e
In order to explore transcriptional regulation mediated by p27, Pax5, and PCAF, Santa Cruz Biotechnology PCAF antibody (Santa Cruz, sc-13124) was used in western blot on human samples (fig 5b) and in western blot on mouse samples (fig s8e). Nucleic Acids Res (2017) ncbi
mouse monoclonal (E-8)
  • immunocytochemistry; human; 1:500; loading ...; fig 3a
In order to characterize the human lysine acetyltransferases KAT2A and KAT2B, Santa Cruz Biotechnology PCAF antibody (Santa Cruz, sc-13124) was used in immunocytochemistry on human samples at 1:500 (fig 3a). Nat Commun (2016) ncbi
mouse monoclonal (E-8)
  • immunoprecipitation; human; loading ...; fig 5i
  • western blot; human; 1:200; loading ...; fig 1b, 4c
Santa Cruz Biotechnology PCAF antibody (Santa Cruz, sc-13124) was used in immunoprecipitation on human samples (fig 5i) and in western blot on human samples at 1:200 (fig 1b, 4c). J Pathol (2016) ncbi
mouse monoclonal (E-8)
  • chromatin immunoprecipitation; human; fig 6
  • western blot; human; fig 3
In order to analyze tamoxifen-resistance in ERalpha-positive breast cancer by degradation of NCoR due to COPS5 amplification and overexpression, Santa Cruz Biotechnology PCAF antibody (Santa Cruz, sc-13124) was used in chromatin immunoprecipitation on human samples (fig 6) and in western blot on human samples (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (E-8)
  • western blot; mouse; 1:1000
Santa Cruz Biotechnology PCAF antibody (Santa Cruz, sc-13124) was used in western blot on mouse samples at 1:1000. Toxicol Appl Pharmacol (2015) ncbi
mouse monoclonal (E-8)
  • chromatin immunoprecipitation; mouse; fig 6
Santa Cruz Biotechnology PCAF antibody (Santa Cruz, sc13124) was used in chromatin immunoprecipitation on mouse samples (fig 6). BMC Biol (2015) ncbi
mouse monoclonal (E-8)
  • western blot; human
Santa Cruz Biotechnology PCAF antibody (Santa Cruz Biotechnology, sc-13124) was used in western blot on human samples . Arthritis Rheumatol (2015) ncbi
mouse monoclonal (E-8)
  • immunoprecipitation; human; fig s1
  • western blot; human; 1:500; fig s1
Santa Cruz Biotechnology PCAF antibody (Santa Cruz Biotechnology, sc-13124) was used in immunoprecipitation on human samples (fig s1) and in western blot on human samples at 1:500 (fig s1). Mol Autism (2014) ncbi
mouse monoclonal (E-8)
  • western blot; mouse; fig 6a
Santa Cruz Biotechnology PCAF antibody (Santa, 13124) was used in western blot on mouse samples (fig 6a). elife (2014) ncbi
mouse monoclonal (E-8)
  • western blot; human
  • chromatin immunoprecipitation; mouse
Santa Cruz Biotechnology PCAF antibody (Santa, sc13124) was used in western blot on human samples and in chromatin immunoprecipitation on mouse samples . PLoS Genet (2014) ncbi
mouse monoclonal (E-8)
  • chromatin immunoprecipitation; human; 3 ug/time
Santa Cruz Biotechnology PCAF antibody (Santa Cruz, sc-13124) was used in chromatin immunoprecipitation on human samples at 3 ug/time. Cell Cycle (2010) ncbi
BioLegend
mouse monoclonal (15G10)
  • immunocytochemistry; human; 1:400; loading ...; fig 1f
BioLegend PCAF antibody (BioLegend, 623402) was used in immunocytochemistry on human samples at 1:400 (fig 1f). J Immunother Cancer (2021) ncbi
mouse monoclonal (15G10)
BioLegend PCAF antibody (BioLegend, 623402) was used . Nat Commun (2017) ncbi
Abcam
domestic rabbit polyclonal
  • ChIP-Seq; human; loading ...; fig 3e
  • immunoprecipitation; human; loading ...; fig s8c
In order to explore transcriptional regulation mediated by p27, Pax5, and PCAF, Abcam PCAF antibody (Abcam, ab12188) was used in ChIP-Seq on human samples (fig 3e) and in immunoprecipitation on human samples (fig s8c). Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4
  • western blot; human; fig 4
Abcam PCAF antibody (Abcam, ab12188) was used in chromatin immunoprecipitation on human samples (fig 4) and in western blot on human samples (fig 4). Nucleic Acids Res (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C14G9)
  • western blot; human; 1:1000; loading ...; fig 6a
Cell Signaling Technology PCAF antibody (Cell Signaling Technology, 3378) was used in western blot on human samples at 1:1000 (fig 6a). Front Oncol (2020) ncbi
domestic rabbit monoclonal (C14G9)
  • immunocytochemistry; mouse; loading ...; fig 4e
  • western blot; mouse; loading ...; fig 4d
Cell Signaling Technology PCAF antibody (Cell Signaling, 3378S) was used in immunocytochemistry on mouse samples (fig 4e) and in western blot on mouse samples (fig 4d). J Clin Invest (2019) ncbi
domestic rabbit monoclonal (C14G9)
  • western blot; human; loading ...; fig 3a
Cell Signaling Technology PCAF antibody (Cell Signaling, 3378) was used in western blot on human samples (fig 3a). Biosci Rep (2018) ncbi
domestic rabbit monoclonal (C14G9)
  • chromatin immunoprecipitation; human; loading ...; fig 5e
In order to assess the effect of TNF-alpha inhibitors on monocyte chemoattractant protein-1, Cell Signaling Technology PCAF antibody (Cell Signaling, 3378) was used in chromatin immunoprecipitation on human samples (fig 5e). Mol Immunol (2017) ncbi
domestic rabbit monoclonal (C14G9)
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology PCAF antibody (Cell Signaling, 3378) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (C14G9)
  • immunoprecipitation; human; loading ...; fig 6a
  • western blot; human; loading ...; fig 6c
In order to examine the mechanisms by which IL-6/STAT3 signaling and TIMP-1 modulate the crosstalk between tumor cells and cancer associated fibroblasts in the tumor microenvironment, Cell Signaling Technology PCAF antibody (Cell Signaling, 3378) was used in immunoprecipitation on human samples (fig 6a) and in western blot on human samples (fig 6c). Cell Signal (2016) ncbi
domestic rabbit monoclonal (C14G9)
  • RNA immunoprecipitation; human; fig 3
  • western blot; human; fig s3
Cell Signaling Technology PCAF antibody (Cell Signaling, 3378S) was used in RNA immunoprecipitation on human samples (fig 3) and in western blot on human samples (fig s3). Genome Biol (2016) ncbi
domestic rabbit monoclonal (C14G9)
  • other; mouse; 1:500; fig s1
In order to identify host signaling dynamics upon Burkholderia spp. infection by a reverse-phase protein microarray-based screen, Cell Signaling Technology PCAF antibody (Cell Signaling, 3378) was used in other on mouse samples at 1:500 (fig s1). Front Microbiol (2015) ncbi
domestic rabbit monoclonal (C14G9)
  • western blot; human; fig 2
Cell Signaling Technology PCAF antibody (Cell Signaling Technologies, 3378S) was used in western blot on human samples (fig 2). Chem Biol (2015) ncbi
domestic rabbit monoclonal (C14G9)
  • western blot; human; 1:1000; fig 1
In order to study stress erythropoiesis regulated by an acetate switch, Cell Signaling Technology PCAF antibody (Cell Signaling Technology, 3378) was used in western blot on human samples at 1:1000 (fig 1). Nat Med (2014) ncbi
domestic rabbit monoclonal (C14G9)
  • chromatin immunoprecipitation; mouse
In order to study the modulation of CD4 T-cell senescence and cytokine production and the roles played by Menin and Bach2, Cell Signaling Technology PCAF antibody (Cell Signaling Technology, 3378) was used in chromatin immunoprecipitation on mouse samples . Nat Commun (2014) ncbi
domestic rabbit monoclonal (C14G9)
  • western blot; mouse
Cell Signaling Technology PCAF antibody (CST, 3378) was used in western blot on mouse samples . J Clin Invest (2013) ncbi
domestic rabbit monoclonal (C14G9)
  • western blot; rat
Cell Signaling Technology PCAF antibody (Cell Signaling, 3378) was used in western blot on rat samples . Neuropsychopharmacology (2010) ncbi
MilliporeSigma
mouse monoclonal (NM11)
  • chromatin immunoprecipitation; mouse; fig 6
MilliporeSigma PCAF antibody (sigma, P2859) was used in chromatin immunoprecipitation on mouse samples (fig 6). Nucleic Acids Res (2016) ncbi
Articles Reviewed
  1. Wu Q, Tian A, Li B, Leduc M, Forveille S, Hamley P, et al. IGF1 receptor inhibition amplifies the effects of cancer drugs by autophagy and immune-dependent mechanisms. J Immunother Cancer. 2021;9: pubmed publisher
  2. Fang M, Zhang M, Wang Y, Wei F, Wu J, Mou X, et al. Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity. Front Oncol. 2020;10:601055 pubmed publisher
  3. Bartolomé A, Zhu C, Sussel L, Pajvani U. Notch signaling dynamically regulates adult β cell proliferation and maturity. J Clin Invest. 2019;129:268-280 pubmed publisher
  4. Zhang Y, Cao Y, Zhang L, Feng C, Zhou G, Wen G. Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR. Biosci Rep. 2018;38: pubmed publisher
  5. Han S, Yang A, Lee S, Lee H, Park C, Park H. Expanding the genetic code of Mus musculus. Nat Commun. 2017;8:14568 pubmed publisher
  6. Perearnau A, Orlando S, Islam A, Gallastegui E, Martinez J, Jordan A, et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res. 2017;45:5086-5099 pubmed publisher
  7. Lin Y, Lin Y, Huang M, Kuo P, Wu C, Lee M, et al. Tumor necrosis factor-alpha inhibitors suppress CCL2 chemokine in monocytes via epigenetic modification. Mol Immunol. 2017;83:82-91 pubmed publisher
  8. Fournier M, Orpinell M, Grauffel C, Scheer E, Garnier J, Ye T, et al. KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification. Nat Commun. 2016;7:13227 pubmed publisher
  9. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  10. Bharathy N, Suriyamurthy S, Rao V, Ow J, Lim H, Chakraborty P, et al. P/CAF mediates PAX3-FOXO1-dependent oncogenesis in alveolar rhabdomyosarcoma. J Pathol. 2016;240:269-281 pubmed publisher
  11. Lu R, Hu X, Zhou J, Sun J, Zhu A, Xu X, et al. COPS5 amplification and overexpression confers tamoxifen-resistance in ER?-positive breast cancer by degradation of NCoR. Nat Commun. 2016;7:12044 pubmed publisher
  12. Zheng X, Xu M, Yao B, Wang C, Jia Y, Liu Q. IL-6/STAT3 axis initiated CAFs via up-regulating TIMP-1 which was attenuated by acetylation of STAT3 induced by PCAF in HCC microenvironment. Cell Signal. 2016;28:1314-24 pubmed publisher
  13. Rao V, Ow J, Shankar S, Bharathy N, Manikandan J, Wang Y, et al. G9a promotes proliferation and inhibits cell cycle exit during myogenic differentiation. Nucleic Acids Res. 2016;44:8129-43 pubmed publisher
  14. G Hendrickson D, Kelley D, Tenen D, BERNSTEIN B, Rinn J. Widespread RNA binding by chromatin-associated proteins. Genome Biol. 2016;17:28 pubmed publisher
  15. Akhade V, Dighe S, Kataruka S, Rao M. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res. 2016;44:387-401 pubmed publisher
  16. Chiang C, Uzoma I, Lane D, Memišević V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
  17. Tyler C, Hafez A, Solomon E, Allan A. Developmental exposure to 50 parts-per-billion arsenic influences histone modifications and associated epigenetic machinery in a region- and sex-specific manner in the adult mouse brain. Toxicol Appl Pharmacol. 2015;288:40-51 pubmed publisher
  18. Montgomery D, Sorum A, Guasch L, Nicklaus M, Meier J. Metabolic Regulation of Histone Acetyltransferases by Endogenous Acyl-CoA Cofactors. Chem Biol. 2015;22:1030-1039 pubmed publisher
  19. Almuzzaini B, Sarshad A, Farrants A, Percipalle P. Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation. BMC Biol. 2015;13:35 pubmed publisher
  20. Zhang Z, Shi L, Song L, Ephrem E, Petri M, Sullivan K. Interferon regulatory factor 1 marks activated genes and can induce target gene expression in systemic lupus erythematosus. Arthritis Rheumatol. 2015;67:785-96 pubmed publisher
  21. Crider A, Thakkar R, Ahmed A, Pillai A. Dysregulation of estrogen receptor beta (ERβ), aromatase (CYP19A1), and ER co-activators in the middle frontal gyrus of autism spectrum disorder subjects. Mol Autism. 2014;5:46 pubmed publisher
  22. Xu M, Nagati J, Xie J, Li J, Walters H, Moon Y, et al. An acetate switch regulates stress erythropoiesis. Nat Med. 2014;20:1018-26 pubmed publisher
  23. Herrera F, Yamaguchi T, Roelink H, Tjian R. Core promoter factor TAF9B regulates neuronal gene expression. elife. 2014;3:e02559 pubmed publisher
  24. Sarshad A, Corcoran M, Al Muzzaini B, Borgonovo Brandter L, von Euler A, Lamont D, et al. Glycogen synthase kinase (GSK) 3? phosphorylates and protects nuclear myosin 1c from proteasome-mediated degradation to activate rDNA transcription in early G1 cells. PLoS Genet. 2014;10:e1004390 pubmed publisher
  25. Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, et al. The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis. Nat Commun. 2014;5:3555 pubmed publisher
  26. Ravnskjaer K, Hogan M, Lackey D, Tora L, Dent S, Olefsky J, et al. Glucagon regulates gluconeogenesis through KAT2B- and WDR5-mediated epigenetic effects. J Clin Invest. 2013;123:4318-28 pubmed publisher
  27. Bousiges O, Vasconcelos A, Neidl R, Cosquer B, Herbeaux K, Panteleeva I, et al. Spatial memory consolidation is associated with induction of several lysine-acetyltransferase (histone acetyltransferase) expression levels and H2B/H4 acetylation-dependent transcriptional events in the rat hippocampus. Neuropsychopharmacology. 2010;35:2521-37 pubmed publisher
  28. Gatta R, Mantovani R. Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters. Cell Cycle. 2010;9:2149-59 pubmed