This is a Validated Antibody Database (VAD) review about human PBRM1, based on 16 published articles (read how Labome selects the articles), using PBRM1 antibody in all methods. It is aimed to help Labome visitors find the most suited PBRM1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PBRM1 synonym: BAF180; PB1; protein polybromo-1; BRG1-associated factor 180; polybromo-1D

Bethyl
rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Bethyl PBRM1 antibody (Bethyl Laboratories, A301-590A) was used in western blot on human samples (fig 2a). J Biol Chem (2018) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1-s1d
Bethyl PBRM1 antibody (Bethyl Laboratories, A301-591A) was used in western blot on human samples at 1:1000 (fig 1-s1d). elife (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
Bethyl PBRM1 antibody (Bethyl Laboratories, A301-591A) was used in immunoprecipitation on human samples (fig 1) and in western blot on human samples (fig 1). PLoS ONE (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
  • western blot; mouse; 1:500; fig 4a
Bethyl PBRM1 antibody (Bethyl Laboratories, A301-591A) was used in chromatin immunoprecipitation on mouse samples and in western blot on mouse samples at 1:500 (fig 4a). Oncotarget (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; human; 1:250; fig s3
Bethyl PBRM1 antibody (Bethyl Laboratories, A301-591A) was used in immunohistochemistry - free floating section on human samples at 1:250 (fig s3). Mod Pathol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:50; fig 3
Bethyl PBRM1 antibody (Bethyl Laboratories, A301-591A) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 3). J Med Genet (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 6
Bethyl PBRM1 antibody (Bethyl, A301-590A) was used in western blot on human samples (fig 6). PLoS Genet (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:250; tbl 1
Bethyl PBRM1 antibody (Bethyl Laboratories, A301-591A) was used in immunohistochemistry - paraffin section on human samples at 1:250 (tbl 1). Histopathology (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:250
Bethyl PBRM1 antibody (Bethyl, A301-591A) was used in immunohistochemistry on human samples at 1:250. Urol Oncol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:250
Bethyl PBRM1 antibody (Bethyl Laboratory, A301-591A) was used in immunohistochemistry - paraffin section on human samples at 1:250. Hum Pathol (2014) ncbi
rabbit polyclonal
  • western blot; human; fig 1a
Bethyl PBRM1 antibody (GE Healthcare, A301-590A) was used in western blot on human samples (fig 1a). Oncogene (2014) ncbi
Novus Biologicals
rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 1a
In order to define distinct functional subgroups of clear cell renal cell carcinoma based on expression of BAF180, Novus Biologicals PBRM1 antibody (Novus Biologicals, NB100-79833) was used in immunohistochemistry on human samples (fig 1b) and in western blot on human samples (fig 1a). Oncogenesis (2017) ncbi
Invitrogen
mouse monoclonal
  • immunoprecipitation; human; 2 ug
In order to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality, Invitrogen PBRM1 antibody (life tech, 730027) was used in immunoprecipitation on human samples at 2 ug. Nat Methods (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; 1:500; loading ...
In order to describe a method to screen for synthetic lethality based on co-culturing two syngeneic cell lines containing individual fluorescent tags, EMD Millipore PBRM1 antibody (Millipore, ABE70) was used in western blot on human samples at 1:500. DNA Repair (Amst) (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human
EMD Millipore PBRM1 antibody (Millipore, ABE70) was used in immunoprecipitation on human samples . PLoS ONE (2012) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunohistochemistry; human; 1:30; fig 4
  • western blot; human; fig 1
Sigma-Aldrich PBRM1 antibody (Sigma, HPA015629) was used in immunohistochemistry on human samples at 1:30 (fig 4) and in western blot on human samples (fig 1). J Pathol (2015) ncbi
Articles Reviewed
  1. Alpsoy A, Dykhuizen E. Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes. J Biol Chem. 2018;293:3892-3903 pubmed publisher
  2. Kelso T, Porter D, Amaral M, Shokhirev M, Benner C, Hargreaves D. Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. elife. 2017;6: pubmed publisher
  3. Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell W, Tan A, et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis. 2017;6:e287 pubmed publisher
  4. Hopkins S, McGregor G, Murray J, Downs J, Savic V. Novel synthetic lethality screening method identifies TIP60-dependent radiation sensitivity in the absence of BAF180. DNA Repair (Amst). 2016;46:47-54 pubmed publisher
  5. Chowdhury B, Porter E, Stewart J, Ferreira C, Schipma M, Dykhuizen E. PBRM1 Regulates the Expression of Genes Involved in Metabolism and Cell Adhesion in Renal Clear Cell Carcinoma. PLoS ONE. 2016;11:e0153718 pubmed publisher
  6. Lee H, Dai F, Zhuang L, Xiao Z, Kim J, Zhang Y, et al. BAF180 regulates cellular senescence and hematopoietic stem cell homeostasis through p21. Oncotarget. 2016;7:19134-46 pubmed publisher
  7. Ho T, Kapur P, Joseph R, Serie D, Eckel Passow J, Tong P, et al. Loss of histone H3 lysine 36 trimethylation is associated with an increased risk of renal cell carcinoma-specific death. Mod Pathol. 2016;29:34-42 pubmed publisher
  8. Macher Goeppinger S, Keith M, Tagscherer K, Singer S, Winkler J, Hofmann T, et al. PBRM1 (BAF180) protein is functionally regulated by p53-induced protein degradation in renal cell carcinomas. J Pathol. 2015;237:460-71 pubmed publisher
  9. Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, et al. Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation. Nat Methods. 2015;12:725-31 pubmed publisher
  10. Benusiglio P, Couvé S, Gilbert Dussardier B, Deveaux S, Le Jeune H, Da Costa M, et al. A germline mutation in PBRM1 predisposes to renal cell carcinoma. J Med Genet. 2015;52:426-30 pubmed publisher
  11. Ceballos Chávez M, Subtil Rodríguez A, Giannopoulou E, Soronellas D, Vázquez Chávez E, Vicent G, et al. The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers. PLoS Genet. 2015;11:e1005174 pubmed publisher
  12. Rao Q, Xia Q, Wang Z, Li L, Shen Q, Shi S, et al. Frequent co-inactivation of the SWI/SNF subunits SMARCB1, SMARCA2 and PBRM1 in malignant rhabdoid tumours. Histopathology. 2015;67:121-9 pubmed publisher
  13. Ho T, Kapur P, Joseph R, Serie D, Eckel Passow J, Parasramka M, et al. Loss of PBRM1 and BAP1 expression is less common in non-clear cell renal cell carcinoma than in clear cell renal cell carcinoma. Urol Oncol. 2015;33:23.e9-23.e14 pubmed publisher
  14. Li L, Fan X, Xia Q, Rao Q, Liu B, Yu B, et al. Concurrent loss of INI1, PBRM1, and BRM expression in epithelioid sarcoma: implications for the cocontributions of multiple SWI/SNF complex members to pathogenesis. Hum Pathol. 2014;45:2247-54 pubmed publisher
  15. Alfonso Pérez T, Domínguez Sánchez M, Garcia Dominguez M, Reyes J. Cytoplasmic interaction of the tumour suppressor protein hSNF5 with dynamin-2 controls endocytosis. Oncogene. 2014;33:3064-74 pubmed publisher
  16. Leung J, Nevins J. E2F6 associates with BRG1 in transcriptional regulation. PLoS ONE. 2012;7:e47967 pubmed publisher